PKRI1_HUMAN
ID PKRI1_HUMAN Reviewed; 184 AA.
AC Q9H875; B4DGM2; Q8NDM6; Q96CF8;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=PRKR-interacting protein 1;
GN Name=PRKRIP1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Thyroid;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT TYR-106.
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-184 (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT networks.";
RL Cell 127:635-648(2006).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8] {ECO:0007744|PDB:5XJC}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.60 ANGSTROMS), FUNCTION, SUBCELLULAR
RP LOCATION, AND SUBUNIT.
RX PubMed=28502770; DOI=10.1016/j.cell.2017.04.033;
RA Zhang X., Yan C., Hang J., Finci L.I., Lei J., Shi Y.;
RT "An Atomic Structure of the Human Spliceosome.";
RL Cell 169:918-929(2017).
RN [9] {ECO:0007744|PDB:6QDV}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.30 ANGSTROMS) OF 20-139, FUNCTION,
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=30705154; DOI=10.1126/science.aaw5569;
RA Fica S.M., Oubridge C., Wilkinson M.E., Newman A.J., Nagai K.;
RT "A human postcatalytic spliceosome structure reveals essential roles of
RT metazoan factors for exon ligation.";
RL Science 363:710-714(2019).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome (PubMed:28502770, PubMed:30705154). Binds double-stranded
CC RNA. Inhibits EIF2AK2 kinase activity (By similarity).
CC {ECO:0000250|UniProtKB:Q9CWV6, ECO:0000269|PubMed:28502770,
CC ECO:0000269|PubMed:30705154}.
CC -!- SUBUNIT: Component of the pre-catalytic and post-catalytic spliceosome
CC complexes (PubMed:28502770, PubMed:30705154). Interacts with EIF2AK2
CC (By similarity). {ECO:0000250|UniProtKB:Q9CWV6,
CC ECO:0000269|PubMed:28502770, ECO:0000269|PubMed:30705154}.
CC -!- INTERACTION:
CC Q9H875; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-744488, EBI-739624;
CC Q9H875; Q12933: TRAF2; NbExp=3; IntAct=EBI-744488, EBI-355744;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28502770,
CC ECO:0000269|PubMed:30705154}. Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9CWV6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H875-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H875-2; Sequence=VSP_056088, VSP_056089;
CC -!- SIMILARITY: Belongs to the PRKRIP1 family. {ECO:0000305}.
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DR EMBL; AK023964; BAB14742.1; -; mRNA.
DR EMBL; AK294664; BAG57833.1; -; mRNA.
DR EMBL; AC005088; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC073517; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC091390; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471197; EAW50241.1; -; Genomic_DNA.
DR EMBL; BC014298; AAH14298.1; -; mRNA.
DR EMBL; AL833837; CAD38697.2; -; mRNA.
DR CCDS; CCDS34714.1; -. [Q9H875-1]
DR RefSeq; NP_078929.1; NM_024653.3. [Q9H875-1]
DR PDB; 5XJC; EM; 3.60 A; X=1-184.
DR PDB; 6ICZ; EM; 3.00 A; X=1-184.
DR PDB; 6QDV; EM; 3.30 A; D=20-139.
DR PDBsum; 5XJC; -.
DR PDBsum; 6ICZ; -.
DR PDBsum; 6QDV; -.
DR AlphaFoldDB; Q9H875; -.
DR SMR; Q9H875; -.
DR BioGRID; 122824; 21.
DR CORUM; Q9H875; -.
DR IntAct; Q9H875; 14.
DR STRING; 9606.ENSP00000419270; -.
DR iPTMnet; Q9H875; -.
DR PhosphoSitePlus; Q9H875; -.
DR BioMuta; PRKRIP1; -.
DR DMDM; 74733776; -.
DR EPD; Q9H875; -.
DR jPOST; Q9H875; -.
DR MassIVE; Q9H875; -.
DR MaxQB; Q9H875; -.
DR PaxDb; Q9H875; -.
DR PeptideAtlas; Q9H875; -.
DR PRIDE; Q9H875; -.
DR ProteomicsDB; 4144; -.
DR ProteomicsDB; 81197; -. [Q9H875-1]
DR Antibodypedia; 35198; 64 antibodies from 17 providers.
DR DNASU; 79706; -.
DR Ensembl; ENST00000397912.3; ENSP00000381010.3; ENSG00000128563.13. [Q9H875-1]
DR Ensembl; ENST00000496391.5; ENSP00000419270.1; ENSG00000128563.13. [Q9H875-1]
DR GeneID; 79706; -.
DR KEGG; hsa:79706; -.
DR MANE-Select; ENST00000397912.3; ENSP00000381010.3; NM_024653.4; NP_078929.1.
DR UCSC; uc003uzh.3; human. [Q9H875-1]
DR CTD; 79706; -.
DR GeneCards; PRKRIP1; -.
DR HGNC; HGNC:21894; PRKRIP1.
DR HPA; ENSG00000128563; Low tissue specificity.
DR MIM; 617458; gene.
DR neXtProt; NX_Q9H875; -.
DR OpenTargets; ENSG00000128563; -.
DR PharmGKB; PA134952162; -.
DR VEuPathDB; HostDB:ENSG00000128563; -.
DR eggNOG; KOG4055; Eukaryota.
DR GeneTree; ENSGT00390000005003; -.
DR HOGENOM; CLU_079129_2_0_1; -.
DR InParanoid; Q9H875; -.
DR OMA; EPSFIMG; -.
DR PhylomeDB; Q9H875; -.
DR TreeFam; TF314382; -.
DR PathwayCommons; Q9H875; -.
DR SignaLink; Q9H875; -.
DR BioGRID-ORCS; 79706; 317 hits in 1090 CRISPR screens.
DR ChiTaRS; PRKRIP1; human.
DR GenomeRNAi; 79706; -.
DR Pharos; Q9H875; Tdark.
DR PRO; PR:Q9H875; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9H875; protein.
DR Bgee; ENSG00000128563; Expressed in sural nerve and 169 other tissues.
DR ExpressionAtlas; Q9H875; baseline and differential.
DR Genevisible; Q9H875; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0003725; F:double-stranded RNA binding; ISS:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB.
DR GO; GO:0004860; F:protein kinase inhibitor activity; ISS:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0042326; P:negative regulation of phosphorylation; ISS:UniProtKB.
DR GO; GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0003014; P:renal system process; IDA:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR009548; Prkrip1.
DR PANTHER; PTHR13507; PTHR13507; 1.
DR Pfam; PF06658; DUF1168; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; mRNA processing;
KW mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..184
FT /note="PRKR-interacting protein 1"
FT /id="PRO_0000324787"
FT REGION 1..50
FT /note="Interaction with EIF2AK2"
FT /evidence="ECO:0000250"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 37..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 51..143
FT /note="Required for RNA-binding"
FT /evidence="ECO:0000250"
FT REGION 120..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 126..138
FT /note="Required for nuclear localization"
FT /evidence="ECO:0000250"
FT COILED 91..152
FT /evidence="ECO:0000255"
FT COMPBIAS 140..154
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..38
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056088"
FT VAR_SEQ 132..184
FT /note="QKLKEKKLLAKKMKLEQKKQEGPGQPKEQGSSSSAEASGTEEEEEVPSFTMG
FT R -> TRSAQGAGVQQLCGGIWNRGGGGSAQFHHGAMTMFATASAWNLARAVTRRERRL
FT FGSFSPARTR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056089"
FT VARIANT 7
FT /note="S -> A (in dbSNP:rs6951185)"
FT /id="VAR_039882"
FT VARIANT 106
FT /note="D -> Y (in dbSNP:rs11556160)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_039883"
FT HELIX 78..141
FT /evidence="ECO:0007829|PDB:6ICZ"
SQ SEQUENCE 184 AA; 20997 MW; 0374DA48DD8D4B22 CRC64;
MASPAASSVR PPRPKKEPQT LVIPKNAAEE QKLKLERLMK NPDKAVPIPE KMSEWAPRPP
PEFVRDVMGS SAGAGSGEFH VYRHLRRREY QRQDYMDAMA EKQKLDAEFQ KRLEKNKIAA
EEQTAKRRKK RQKLKEKKLL AKKMKLEQKK QEGPGQPKEQ GSSSSAEASG TEEEEEVPSF
TMGR
