ID   PKRI1_PONAB             Reviewed;         184 AA.
AC   Q5R5J3;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=PRKR-interacting protein 1;
GN   Name=PRKRIP1;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC       spliceosome (By similarity). Binds double-stranded RNA. Inhibits
CC       EIF2AK2 kinase activity (By similarity). {ECO:0000250|UniProtKB:Q9CWV6,
CC       ECO:0000250|UniProtKB:Q9H875}.
CC   -!- SUBUNIT: Component of the pre-catalytic and post-catalytic spliceosome
CC       complexes (By similarity). Interacts with EIF2AK2 (By similarity).
CC       {ECO:0000250|UniProtKB:Q9CWV6, ECO:0000250|UniProtKB:Q9H875}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H875}. Nucleus,
CC       nucleolus {ECO:0000250|UniProtKB:Q9CWV6}.
CC   -!- SIMILARITY: Belongs to the PRKRIP1 family. {ECO:0000305}.
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DR   EMBL; CR860865; CAH92973.1; -; mRNA.
DR   RefSeq; NP_001126756.1; NM_001133284.1.
DR   AlphaFoldDB; Q5R5J3; -.
DR   SMR; Q5R5J3; -.
DR   STRING; 9601.ENSPPYP00000019568; -.
DR   Ensembl; ENSPPYT00000020339; ENSPPYP00000019568; ENSPPYG00000017454.
DR   GeneID; 100173758; -.
DR   KEGG; pon:100173758; -.
DR   CTD; 79706; -.
DR   eggNOG; KOG4055; Eukaryota.
DR   GeneTree; ENSGT00390000005003; -.
DR   HOGENOM; CLU_079129_2_0_1; -.
DR   InParanoid; Q5R5J3; -.
DR   OMA; EPSFIMG; -.
DR   OrthoDB; 1545283at2759; -.
DR   TreeFam; TF314382; -.
DR   Proteomes; UP000001595; Chromosome 7.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0003725; F:double-stranded RNA binding; ISS:UniProtKB.
DR   GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB.
DR   GO; GO:0004860; F:protein kinase inhibitor activity; ISS:UniProtKB.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0042326; P:negative regulation of phosphorylation; ISS:UniProtKB.
DR   GO; GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR009548; Prkrip1.
DR   PANTHER; PTHR13507; PTHR13507; 1.
DR   Pfam; PF06658; DUF1168; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..184
FT                   /note="PRKR-interacting protein 1"
FT                   /id="PRO_0000324789"
FT   REGION          1..50
FT                   /note="Interaction with EIF2AK2"
FT                   /evidence="ECO:0000250"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          37..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          51..143
FT                   /note="Required for RNA-binding"
FT                   /evidence="ECO:0000250"
FT   REGION          120..184
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          126..138
FT                   /note="Required for nuclear localization"
FT                   /evidence="ECO:0000250"
FT   COILED          91..152
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        140..154
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   184 AA;  20997 MW;  0374DA48DD8D4B22 CRC64;
     MASPAASSVR PPRPKKEPQT LVIPKNAAEE QKLKLERLMK NPDKAVPIPE KMSEWAPRPP
     PEFVRDVMGS SAGAGSGEFH VYRHLRRREY QRQDYMDAMA EKQKLDAEFQ KRLEKNKIAA
     EEQTAKRRKK RQKLKEKKLL AKKMKLEQKK QEGPGQPKEQ GSSSSAEASG TEEEEEVPSF
     TMGR