ID   PKS28_DICDI             Reviewed;        2690 AA.
AC   B0G170;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Probable polyketide synthase 28;
DE            Short=dipks28;
DE            EC=2.3.1.-;
GN   Name=pks28; ORFNames=DDB_G0290469;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=17660200; DOI=10.1093/bioinformatics/btm381;
RA   Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H.,
RA   Hranueli D.;
RT   "Polyketide synthase genes and the natural products potential of
RT   Dictyostelium discoideum.";
RL   Bioinformatics 23:2543-2549(2007).
CC   -!- FUNCTION: Probable polyketide synthase. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 phosphopantetheine covalently. {ECO:0000250};
CC   -!- DOMAIN: Modular protein that is responsible for the completion of one
CC       condensation-processing cycle. The beta-ketoacyl synthase region is
CC       responsible for the actual condensation reaction while the acyl/malonyl
CC       transferase region is responsible for incorporating carboxylic acids
CC       units onto an acyl carrier protein (ACP) domain (By similarity).
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: Encoded by one of the numerous copies of polyketide
CC       synthase genes and clustered as a pair pks27/pks28 in chromosome 5.
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DR   EMBL; AAFI02000163; EDR41037.1; -; Genomic_DNA.
DR   RefSeq; XP_001733033.1; XM_001732981.1.
DR   AlphaFoldDB; B0G170; -.
DR   SMR; B0G170; -.
DR   STRING; 44689.DDB0235222; -.
DR   PaxDb; B0G170; -.
DR   PRIDE; B0G170; -.
DR   EnsemblProtists; EDR41037; EDR41037; DDB_G0290469.
DR   GeneID; 8627661; -.
DR   KEGG; ddi:DDB_G0290469; -.
DR   dictyBase; DDB_G0290469; pks28.
DR   eggNOG; KOG1202; Eukaryota.
DR   HOGENOM; CLU_000022_31_5_1; -.
DR   InParanoid; B0G170; -.
DR   OMA; RNWIGAY; -.
DR   PhylomeDB; B0G170; -.
DR   PRO; PR:B0G170; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   Gene3D; 3.10.129.110; -; 1.
DR   Gene3D; 3.40.366.10; -; 1.
DR   Gene3D; 3.40.47.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR001227; Ac_transferase_dom_sf.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR013217; Methyltransf_12.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR032821; PKS_assoc.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR042104; PKS_dehydratase_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR016039; Thiolase-like.
DR   Pfam; PF00698; Acyl_transf_1; 1.
DR   Pfam; PF16197; KAsynt_C_assoc; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   Pfam; PF08659; KR; 1.
DR   Pfam; PF08242; Methyltransf_12; 1.
DR   Pfam; PF14765; PS-DH; 1.
DR   SMART; SM00827; PKS_AT; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 2.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53901; SSF53901; 1.
DR   SUPFAM; SSF55048; SSF55048; 1.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Phosphopantetheine; Phosphoprotein; Reference proteome;
KW   Transferase.
FT   CHAIN           1..2690
FT                   /note="Probable polyketide synthase 28"
FT                   /id="PRO_0000369419"
FT   DOMAIN          2594..2671
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   REGION          168..221
FT                   /note="Beta-ketoacyl synthase"
FT   REGION          651..684
FT                   /note="Acyl/malonyl transferases"
FT   REGION          916..946
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1401..1429
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          906..934
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        187
FT                   /note="For beta-ketoacyl synthase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT   ACT_SITE        661
FT                   /note="For acyl/malonyl transferase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT   MOD_RES         2631
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ   SEQUENCE   2690 AA;  305894 MW;  938E7F10BAD7C5CA CRC64;
     MEKYDIYYNS NEEYYGDVAV IGIGLRFPSG DLNESISKPN QLFNSLLNGL NGIVTTSERW
     SDNYYLNGEI NSVSAGLLPL DEWKRFDPIF FGINPSYDNV VTIDPQQRLL LKCVWEALED
     SGIDPISLRG TNTSTFIGSS TTDYGSLQKS PFETQNNIFG SSNHSVANRI GYNFDFRGEN
     FTIDSACSSS LNAINCGYNS IKSNKSNVSV VGGVNFILDP HVSKSFTQLN MLSPTGKCHS
     FSSDADGFVR SEGVGIVILK KLKDAIKDSN NIYCVIKGSS SNVDGNYDKL NFYSPSKSSQ
     YENMKLAIKS TNGQINESDI DYCEAHGTGT PTGDPIELEG ISRLFDQNNN NKKQVLVGSI
     KSNIGHTEAC SGVASLIKCC IMFKNKLFLQ NINFKESNPL INFKEWALKV VTEPITFNEN
     KTTVMLINNF GVTGSNVCLI LSEFKDKRYS NDESSSDNFC EQIDIDSKAN EKKKFLIPLS
     SNSSTSLDNY KSIIVNNNDD DSNSSTRSFQ EFVYNQIKFK STSLIQKSVI IASDWNEFQD
     DDNQIKLKNS ESLISNITVE KKKSPLTVIV FCGQGSQYNR MALSLYENEP IFRESVNRFD
     KELFKYYGYS VLDRLRSVSD KDEISIHLPI LAQPANIMIQ ISLYELYKHW GVSADIIVGH
     SLGELSSSYS SGMIDFETLC HLIYHRSLAQ NKTTGTGRAL SVNISYDEFI ERYQSKNNKY
     ETLEIACYNS PTSIVIAGKE DLLNEISKEF KSNDIFCAML GSLSSFHTSS QLMIKDEVCS
     LVFKSKLPSV PVFSTVTTNL FNDQTPYNAN YVWENIRQPV SFTQTISNLY KHIESNDMGN
     EITFIEVAPH PTLQFYLNQM KSTQSSYFNN GKSVTIYSPL HKKKNDYNEF LKTISLLYVN
     NNFNINFKSQ LTNINNNNNN INNNNNNNNN NNNNNNNNNN NNNNNNKIIQ FNINSLPLYQ
     WDDNEYFKLN PFHEKITNEG PSIQNLGNGI DSACPTYQTF IDIKKPPFQW LKGHQVSDKF
     YYPGMGYVQN LLSIYPNQDI TISSLEFKSP LVLTEGNNQC LETTVSLLSK NEFNVKSHYK
     DQKTNQWILS SLGNFSLFKH NSINSEKLIN IQALKDKCNF TTISKHDFYE SIKIKTNLTY
     KGLFQGVKEC SIGNNCSLAV VSLNEINNHT ISNHSTIGRS LFNAATLDSC LHGSLIAVAQ
     PVVLDRIEGF KLYSSNIPLS SSLSKDDNDN SNNSLIKELY IFTEEKARTN YQSFSASVKI
     ILPNGRLLME ISRVVCSSVS LANPSNTIIC KPPSNEIYTP YLQPKDSIIN KPQQFKHLYS
     VDEFIAKEED NQIISTELLL SLFYKHINVR CPTINLESLT TLEYNQFKQL YYNNNGLVNE
     NLFKFVFEIL KSYSSSNHYI LNHHNNSENK NKNNNNNNNS NNNENSNNES PIHFEKLYNL
     YTKTTKIIAK QIFPLKDDSF TDTPQSLFEN GFLDDFYKNS RVVQPLNNLL SEIIIEALKP
     ILNQPIVFRI LEAGGGTGSL SLLILEKICK LLNANPNSVI DIEFTWSDVS SSFSAEIKEK
     FSPFTAHKNF NIIHRVLDLE KPLFDQDFKT SYYDLVVMSN VMHVVKKLKP TLDEIHNILT
     PNGQLLFVEP PYKSINYDSV FCCFSQWWPS SDSDTELRPD RSCMNQDKWI KILNETNYRD
     TIISGNDNLI FLIQTRKPSI NEIISKQSSD SSLDQFNSFN NIILFGNNNY GCSLQNSISS
     NQELKSKTIN INNFNEFQTW IANNYDNSDD FDNNKTLIIF LKSIEPINIS NFKEITYEYI
     QINQLILKLE LTNNFKHLLI SLDSTTDNYL SSSIIGAARY FVEYPQLDLY ILNYDIISLK
     ILNNNSSSSC NSSNGSISSC SCKQQQLSLI NYLINTNNNI QKEFTINNNK VYYERYTRHS
     NKIKCNLQSK SFETNKDNLL IQLDSNLEYQ LYSKRVEPNS KEVEIEIKAT GINYKDYLMH
     IGMVSSDLDL KYGKEYEVEN CIGIENPMIG NDFSGIITRL GSDAEKKKFK VGDHVCGVAS
     KTSGSHVVID YNFIYHQPLN YNHSISASIP SIYVTSLHSI YGVGNLKSNE SILIHSAAGG
     IGISSLDLLK CKKHQGHIFL TVGSKDKEDY LKKNYGSFIT AIYSSRNKDY VNEIKNKLIE
     LGEVKQQGVD LILNTLSSEF MDSNFQCLNM SGRIVDLSVT HLTPNDYIAN NHFKYNMGYN
     NVEMIDFNGK MVRSYLKKII KMINSNKLEL SIPIIEYSNN QFKDAIEYIN QRKHIGKIIV
     NHNQDEFNRV YNNYQQNNNN NNQIIMKHSY DISKLNMGKN ILLTGQTGII LEIMKYLIRY
     SNHSIQNIII LSKSKLKWEL ELLINQTKFI KDNIIKFHFI QIDIEDSNKV NQVLNQLELN
     ENITNIDSII HFAFNNDIGD VQDVNMNRLN IAHGAKTIGA INLHNESINR SWKIKQFIIA
     SSVSSIFGSD QQCCYVSACS VIDSLSKYRH SLGLPSLAIN LGTVASTGFI SRNNAIETMF
     KSSFLKLFSP QLVISSLDLF IQNQRQYPNY SLVDFNFEVM LTSPNYHLYK LDYEINIFKK
     SYQINTNSSS GSGSDNEFIH STILNKISEL LSIDESKINE DLQLTQYGMD SLVIVQLKNF
     IDNQLGHNLI TIHQLQHNKI NQSIDIIKFG YLINKNKFKY KNNNKNNNNG