PKS31_DICDI
ID PKS31_DICDI Reviewed; 2623 AA.
AC Q54FQ1;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Probable polyketide synthase 31;
DE Short=dipks31;
DE EC=2.3.1.-;
GN Name=pks31; ORFNames=DDB_G0290703;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP IDENTIFICATION.
RX PubMed=17660200; DOI=10.1093/bioinformatics/btm381;
RA Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H.,
RA Hranueli D.;
RT "Polyketide synthase genes and the natural products potential of
RT Dictyostelium discoideum.";
RL Bioinformatics 23:2543-2549(2007).
CC -!- FUNCTION: Probable polyketide synthase. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000250};
CC Note=Binds 1 phosphopantetheine covalently. {ECO:0000250};
CC -!- DOMAIN: Modular protein that is responsible for the completion of one
CC condensation-processing cycle. The beta-ketoacyl synthase region is
CC responsible for the actual condensation reaction while the acyl/malonyl
CC transferase region is responsible for incorporating carboxylic acids
CC units onto an acyl carrier protein (ACP) domain (By similarity).
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Encoded by one of the numerous copies of polyketide
CC synthase genes and clustered as a quartet pks29/pks30/pks31/pks32 in
CC chromosome 5.
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DR EMBL; AAFI02000167; EAL62086.1; -; Genomic_DNA.
DR RefSeq; XP_635590.1; XM_630498.1.
DR AlphaFoldDB; Q54FQ1; -.
DR SMR; Q54FQ1; -.
DR STRING; 44689.DDB0235264; -.
DR PaxDb; Q54FQ1; -.
DR PRIDE; Q54FQ1; -.
DR EnsemblProtists; EAL62086; EAL62086; DDB_G0290703.
DR GeneID; 8627787; -.
DR KEGG; ddi:DDB_G0290703; -.
DR dictyBase; DDB_G0290703; pks31.
DR eggNOG; KOG1202; Eukaryota.
DR HOGENOM; CLU_000022_31_5_1; -.
DR InParanoid; Q54FQ1; -.
DR OMA; AFYHPDH; -.
DR PhylomeDB; Q54FQ1; -.
DR PRO; PR:Q54FQ1; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR Gene3D; 1.10.1200.10; -; 1.
DR Gene3D; 3.10.129.110; -; 1.
DR Gene3D; 3.40.366.10; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR SUPFAM; SSF51735; SSF51735; 2.
DR SUPFAM; SSF52151; SSF52151; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR SUPFAM; SSF53901; SSF53901; 1.
DR SUPFAM; SSF55048; SSF55048; 1.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 3: Inferred from homology;
KW Phosphopantetheine; Phosphoprotein; Reference proteome; Transferase.
FT CHAIN 1..2623
FT /note="Probable polyketide synthase 31"
FT /id="PRO_0000371391"
FT DOMAIN 2524..2601
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..233
FT /note="Beta-ketoacyl synthase"
FT REGION 666..699
FT /note="Acyl/malonyl transferase"
FT REGION 2600..2623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 199
FT /note="For beta-ketoacyl synthase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT ACT_SITE 676
FT /note="For acyl/malonyl transferase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT MOD_RES 2561
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 2623 AA; 298926 MW; E4686CC74E9BF5B3 CRC64;
MTQNIDNNNN KLIRDRNDDD DVDRNDDGDV AVIGIGLRFP SGNLKESISK PNQLFNELLN
GLDGIVSTSE RWSDNYYLNG EIVSKFAGLL PLDEWKQFDP IFFAINQTYD NVSSIDPQQR
LLLKCVWEAL EDSGIDPISL RGTNTSTFIG NSTIDYYNLQ RSPSETQNNI FGSSTHSIAN
RIGYCFDFRG ENLTIDTACS SSSNAINCGY NSIKTNRSNV SIVGGVNIIL DPHISKSFTQ
LDMLSPTGKC HTFSSDADGF VRSEGVGIVV LKKLKDAIKD SNNIYCVIKG SSSNIDGNFD
KLNFYSPSKS SQCENIKLAI KSTNGQINES DIDYCETHGT GTPTGDPIEL EGISRVFNSA
KIPSTTINNN KQVLVGSIKS NIGHLEACSG VASLIKCCLM FKNKLFLQNI NFKEPNPLIN
FKEWGLKVVT EPIKFNENKS TVMLVNNFGI TGSNVCLILS ELKKNHHNRY ENEYHKIEID
SKVNEKKKYL IPLSSNSSTS LNNYKSSIIK LSNSNSSPTT TTSFKELVHN QIKFKSTSLI
QKSVIIASDW NEFQDENNQI KLENSDNLIS NITVEKKKSP ITVMVLCGQG SQYNKMALSL
YDNEPIFRES VNRFDKELFK YYGYSVLDKL RSIDDKDLIS IHQPILAQPA NVIIQVSLYE
LYKHWGVSAD IIIGHSLGEV SSPYCSGMID FQTLCYLIYH RSVAQNRTTG TGRMLSVNIS
SDEFINNYQS TTKYESLEIA CYNSPTSIVI AGREDLLNEI TNEFKSNNIF CTMLGLLSSF
HTSSQQMIKD EVCSLNISSK QPSIAVFSTV TTNLFNHQSL PFNADYAFEN IRQPVRFTQT
ITNLYKHIES NDMGNEITFI EVSPHPTLQY YLNQMKSTQS SYFNNGKNIT IYSPLNKKKN
DYNEFLKTIS LLYVNNNFDI NFKSQLINDN NNISNTTKLN NLPLYQWDDK EYFKIASFHE
KIKSEGPSIH SLGNNTDSPY PSYQTFIDIK KSPFQWLKGH QVSDKFYYPG MGYVHNLLSI
YPNQDITISS LEFKSPLVLT EGNNQCLQTT IAPLSKNEFN IKSHYKDQKT NQWILSSLGN
FNLTKHNSII SNKLINIQSL KDKCNFTSIS KQDFYETIRI KTNLTYKGLF QGVKQCYIGN
NCSLSIVSLN EIYNQKEYNH LINNSNMNKF LNTAILDTCL HGSLVAVTQP VVLDRIEGFK
YYFSNIPSLN ENNNNDDIKE LYVFSDIKPR TNYQTYSISV KIILPNGTLL VDISNVVCAL
VSLGSNPDST IICKPPLNDI YTPYLQSKDS VVNKPEQFKH LYSVDEFSVN EEDNQFISNE
LLLSLFYKHI NNRCPSINLE SLTTLEYNQF KQLYYNSLVN ENLFKFIFEN LKRYSNILNH
DNNQSNIKPK IEKIFIRTTK IMAKQLFPLK DDDSITDTPQ SLFESGFLDD FYKNSRVVQP
LNNLLSEIIV ETLKPILNEP IVFRILEAGG GTGSLSLLIL EKICKLLNDN STTSIINIEF
TWSDVSASFF AEIKEKFSSF TNHNNLNIIY RVLDLEKPLL DQDLKASYYD FVVMSNVMHV
VKKLKPTLNE IHNILTPNGQ LLYIEPPYKS FYFDSIFGCF SQWWPSSDGD IELRPDRCCM
KQEKWVNLLS QCNYRDTIMS GNDNLLFLIQ TRKPTINEII SEQSISLDQL NSFNNIILFS
NNKNRNSCSS SILDLIRLNQ ELKHKIININ NYNEFQSWIT NNQNKDNCNK SLIIFLKSIE
STMNTSNFKE ITFEYIQINQ LILKLELSNN FKHLLLSLNS TTDNYLSSSI IGAARYFVEF
PQLDLYILNY DNVSIENNQQ LSLINYLINP NNNIQKEFTI NNNKVYYERY CRRSNNIKSK
FQSESFETNK DNLYIQLNSN IEYQLYSKKD ELNSNEVEIE VKATGINYKD YLMYIGMIGS
DLDIKYGKEI EYGIGIDNPK IGNDFSGIIT RLGSNVKKFK VGDQVCGFGS KTNSSHVIID
FNSIYYKPLN CSHSVSASIP SIYITTLHSI YSIGNLKSNE SILIHSAAGG IGISSLDLLK
SKQHQGYIFL TVGSKDKEEY LINKYGSLIT AIYSSRNKDY VYEIKNKLIE LGVVEQHQQG
VDLILNTLSS EYMDSNFQCL NLSGRIVDLS ITHLTPNDYM TNNHYKFNMN YGNVDIEDFP
SKLIKRYLKK IIKMINSNKL ELSVPIIEYS NNQFKDAIEY INQRKHIGKI IVNHNQDEFN
RVYNNYQSNN NQIIMKHSYD ISKLNIGKNI LLTGQTGIVL EILKYLIKYS NHSIENIIIL
SKSKLKWELK LLINQSKFKK DNNIKFHFNQ IDIEDSNKVN QVLNQLELNE NITNIDSIIH
FAFMIDIGDV QQVDMNRLNN AHGAKTIGAI NLHNQSINRS WNIKQFIMAS SVVSIFGSDQ
QCCYVSACSV IDSLSKYRHS IGLPSLAINL GAISSTGFVS RNNAIETMFK SSIVNLFSPQ
LVISSLDLFI QNQHQYPNYC LSDFNFEVLT PTLTNQYHSK FDYQINIVKK SNRIKSFSSG
NSGANNEIIH STILNKISEL LSIDESKINE DLQLSQYGMD SLVIVQLKNF IDNQLGHNII
TIQQLQNNKI NQSIEIIKSA KNNNKNNNNN NNKNNSNNKN KNN