ID   PKS3_RUBID              Reviewed;         391 AA.
AC   Q9AU09;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Polyketide synthase 3;
DE            Short=RiPKS3;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase PKS3;
GN   Name=PKS3;
OS   Rubus idaeus (Raspberry).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Rosoideae; Rosoideae incertae sedis;
OC   Rubus.
OX   NCBI_TaxID=32247;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=cv. Royalty;
RX   PubMed=11437245; DOI=10.1023/a:1010642517738;
RA   Zheng D., Schroder G., Schroder J., Hrazdina G.;
RT   "Molecular and biochemical characterization of three aromatic polyketide
RT   synthase genes from Rubus idaeus.";
RL   Plant Mol. Biol. 46:1-15(2001).
CC   -!- FUNCTION: Polyketide synthase producing p-coumaryltriacetic acid
CC       lactone (CTAL) and slightly naringenin chalcone. Can use p-coumaryl-CoA
CC       as substrate. {ECO:0000269|PubMed:11437245}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023,
CC         ECO:0000269|PubMed:11437245};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF292369; AAK15176.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AU09; -.
DR   SMR; Q9AU09; -.
DR   BioCyc; MetaCyc:MON-15028; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Transferase.
FT   CHAIN           1..391
FT                   /note="Polyketide synthase 3"
FT                   /id="PRO_0000424288"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   391 AA;  42822 MW;  12509CE11AC31559 CRC64;
     MVTVDEVRKA QRAEGPATIL AIGTATPPNC VDQSTYPDYY FRITKSEHRT ELKEKFQRMC
     DKSRIKKRYM YLTEEILKEN PSMCEYMAPS LDARQDMVVV EIPKLGKEAA TKAIKEWGQL
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLAVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AIIVGSDPLP DIERPLFELV
     SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFKPL DITDWNSLFW
     IAHPGGPAIL DQVEAKLGLK PEKLEATRNI LSEYGNMSSA CVLFILDEVR RKSVANGHKT
     TGEGLEWGVL FGFGPGLTVE TVVLHSVAAS T