ID   PKS4_ALOAR              Reviewed;         403 AA.
AC   C4NF90;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Octaketide synthase 2;
DE            Short=OKS 2;
DE            EC=2.3.1.-;
DE   AltName: Full=Polyketide synthase 4;
GN   Name=PKS4;
OS   Aloe arborescens (Kidachi aloe).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Asphodelaceae;
OC   Asphodeloideae; Aloe.
OX   NCBI_TaxID=45385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=19348024; DOI=10.1111/j.1742-4658.2009.06971.x;
RA   Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H.,
RA   Abe I.;
RT   "Novel type III polyketide synthases from Aloe arborescens.";
RL   FEBS J. 276:2391-2401(2009).
CC   -!- FUNCTION: Catalyzes the iterative condensations of 8 molecules of
CC       malonyl-CoA to produce aromatic octaketides, SEK4 and SEK4b, the
CC       products of the minimal polyketide synthase for the
CC       benzoisochromanequinone actinorhodin. May be involved in the
CC       biosynthesis of the octaketide barbaloin.
CC       {ECO:0000269|PubMed:19348024}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: A.arborescens is a medicinal plant rich in aromatic
CC       polyketides, such as pharmaceutically important aloenin (hexaketide),
CC       aloesin (heptaketide) and barbaloin (octaketide).
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; FJ536166; ACR19997.1; -; mRNA.
DR   AlphaFoldDB; C4NF90; -.
DR   SMR; C4NF90; -.
DR   BioCyc; MetaCyc:MON-15018; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IDA:UniProtKB.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
PE   1: Evidence at protein level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..403
FT                   /note="Octaketide synthase 2"
FT                   /id="PRO_0000422577"
FT   ACT_SITE        174
FT                   /evidence="ECO:0000250"
FT   BINDING         281
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   BINDING         318..321
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   SITE            207
FT                   /note="Determines the polyketide chain length and product
FT                   specificity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   403 AA;  44385 MW;  3E6BC10D7D40A863 CRC64;
     MGSLSNYSPV MEDVQAIRKA QKADGTATVM AIGTAHPPHI FPQDTYADFY FRATNSEHKV
     ELKKKFDRIC KKTMIGKRYF NYDEEFLKKY PNITSFDEPS LNDRQDICVP GVPALGAEAA
     VKAIAEWGRP KSEITHLVFC TSCGVDMPSA DFQCAKLLGL RTNVNKYCVY MQGCYAGGTV
     MRYAKDLAEN NRGARVLVVC AELTIIGLRG PNESHLDNAI GNSLFGDGAA ALIVGSDPII
     GVERPMFEIV CAKQTVIPNS EDVIHLHMRE AGLMFYMSKD SPETISNNVE ACLVDVFKSV
     GMTPPEDWNS LFWIPHPGGR AILDQVEARL KLRPEKFGAT RTVLWDCGNM VSACVLYILD
     EMRRKSVADG LATYGEGLEW GVLLGFGPGM TVETILLHSL PPV