ID   PKS5_ALOAR              Reviewed;         405 AA.
AC   C4NF91;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Octaketide synthase 3;
DE            Short=OKS 3;
DE            EC=2.3.1.-;
DE   AltName: Full=Polyketide synthase 5;
GN   Name=PKS5;
OS   Aloe arborescens (Kidachi aloe).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Asphodelaceae;
OC   Asphodeloideae; Aloe.
OX   NCBI_TaxID=45385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=19348024; DOI=10.1111/j.1742-4658.2009.06971.x;
RA   Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H.,
RA   Abe I.;
RT   "Novel type III polyketide synthases from Aloe arborescens.";
RL   FEBS J. 276:2391-2401(2009).
CC   -!- FUNCTION: Catalyzes the iterative condensations of 8 molecules of
CC       malonyl-CoA to produce aromatic octaketides, SEK4 and SEK4b, the
CC       products of the minimal polyketide synthase for the
CC       benzoisochromanequinone actinorhodin. May be involved in the
CC       biosynthesis of the octaketide barbaloin.
CC       {ECO:0000269|PubMed:19348024}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: A.arborescens is a medicinal plant rich in aromatic
CC       polyketides, such as pharmaceutically important aloenin (hexaketide),
CC       aloesin (heptaketide) and barbaloin (octaketide).
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; FJ536167; ACR19998.1; -; mRNA.
DR   AlphaFoldDB; C4NF91; -.
DR   SMR; C4NF91; -.
DR   PRIDE; C4NF91; -.
DR   BioCyc; MetaCyc:MON-15019; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IDA:UniProtKB.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
PE   1: Evidence at protein level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..405
FT                   /note="Octaketide synthase 3"
FT                   /id="PRO_0000422578"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        176
FT                   /evidence="ECO:0000250"
FT   BINDING         283
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   BINDING         320..323
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   SITE            209
FT                   /note="Determines the polyketide chain length and product
FT                   specificity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   405 AA;  44633 MW;  545DB81D3E6DBA00 CRC64;
     MGSIAESSPL MSRENVEGIR KAQRAEGTAT VMAIGTAHPP HIFPQDTYAD FYFRATNSEH
     KVELKKKFDR ICKKTMIGKR YFNYDEEFLK KYPNITSFDE PSLNDRQDIC VPGVPALGKE
     AALKAIEEWG QPLSKITHLV FCTSCGVDMP SADFQLAKLL GLNTNVNKYC VYMQGCYAGG
     TVLRYAKDLA ENNRGSRVLV VCAELTIIGL RGPNESHLDN AIGNSLFGDG AAALIVGADP
     IVGIEKPIFE IVCAKQTVIP DSEDVIHLHL REAGLMFYMS KDSPETISNN VEGCLVDIFK
     SVGMTPPADW NSLFWIPHPG GRAILDEVEA RLKLRPEKFR ATRHVLWEYG NMVSACVLYI
     LDEMRNKSAA DGLGTYGEGL EWGVLLGFGP GMTVETILLH SLPPV