PKS5_DICDI
ID PKS5_DICDI Reviewed; 2512 AA.
AC Q86JI5; Q55AY9;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Probable polyketide synthase 5;
DE Short=dipks5;
DE EC=2.3.1.-;
GN Name=pks5; ORFNames=DDB_G0271520;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION.
RX PubMed=17660200; DOI=10.1093/bioinformatics/btm381;
RA Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H.,
RA Hranueli D.;
RT "Polyketide synthase genes and the natural products potential of
RT Dictyostelium discoideum.";
RL Bioinformatics 23:2543-2549(2007).
RN [4]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
CC -!- FUNCTION: Probable polyketide synthase. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000250};
CC Note=Binds 1 phosphopantetheine covalently. {ECO:0000250};
CC -!- INDUCTION: Up-regulated by P.aeruginosa, PAO1 strain and PA14 strain
CC infection. {ECO:0000269|PubMed:18590548}.
CC -!- DOMAIN: Modular protein that is responsible for the completion of one
CC condensation-processing cycle. The beta-ketoacyl synthase region is
CC responsible for the actual condensation reaction while the acyl/malonyl
CC transferase region is responsible for incorporating carboxylic acids
CC units onto an acyl carrier protein (ACP) domain (By similarity).
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Encoded by one of the numerous copies of polyketide
CC synthase genes and clustered as a quintuplet pks5/pks6/pks7/pks8/pks9
CC in chromosome 2.
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DR EMBL; AAFI02000006; EAL71624.1; -; Genomic_DNA.
DR RefSeq; XP_645557.1; XM_640465.1.
DR AlphaFoldDB; Q86JI5; -.
DR SMR; Q86JI5; -.
DR STRING; 44689.DDB0235300; -.
DR PaxDb; Q86JI5; -.
DR EnsemblProtists; EAL71624; EAL71624; DDB_G0271520.
DR GeneID; 8618014; -.
DR KEGG; ddi:DDB_G0271520; -.
DR dictyBase; DDB_G0271520; pks5.
DR eggNOG; KOG1202; Eukaryota.
DR HOGENOM; CLU_000022_31_0_1; -.
DR InParanoid; Q86JI5; -.
DR PhylomeDB; Q86JI5; -.
DR PRO; PR:Q86JI5; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR Gene3D; 3.10.129.110; -; 1.
DR Gene3D; 3.40.366.10; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR SUPFAM; SSF51735; SSF51735; 2.
DR SUPFAM; SSF52151; SSF52151; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR SUPFAM; SSF53901; SSF53901; 1.
DR SUPFAM; SSF55048; SSF55048; 1.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 2: Evidence at transcript level;
KW Phosphopantetheine; Phosphoprotein; Reference proteome; Transferase.
FT CHAIN 1..2512
FT /note="Probable polyketide synthase 5"
FT /id="PRO_0000376880"
FT DOMAIN 2430..2507
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 168..221
FT /note="Beta-ketoacyl synthase"
FT REGION 638..671
FT /note="Acyl/malonyl transferase"
FT ACT_SITE 187
FT /note="For beta-ketoacyl synthase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT ACT_SITE 648
FT /note="For acyl/malonyl transferase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT MOD_RES 2467
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 2512 AA; 285270 MW; 1920B530724E3CE1 CRC64;
MDMKLNDIEN ENFINQMKGV AIVGIGFRIP SGNSENSISS PDDLFNNLKN GFDGVSSTSE
RWSDNYHKLG EISSPNAGLL PLNEWKSFDP LFFGINPSDA HHIDPQQRLL LKCTWEALED
ASIDPISIRG TNTSVFIGSS TIDYLHANKH PDLILKNAIG FSPSTLSNRI SYCFDIHGPS
LSIDTACSSS MNAVTQGYHS ILNGTSNMSI VGGVNFVIDV ETIKGFSYLN MLSKTHGKCK
AFDESGDGFT RGECAGVVVL KKLDDAIRDG NRIYCIINGI SSNIDGNGIA DKANFYSPSK
QSQFNNINLA FKSTNGKISV NDIQYIEAHG TGTKTGDPIE TEAISMAFKN RDKSTPILIG
SIKSNIGHCE AGSGVVSLIK CCLMFKYQCF LPNIHFKNPN PLIKFNEWNL KVVTSPIPFN
RNNEKSVSMM INNFGVTGSN CCLLISEFKK QDYESYENNI GSINKNILVP FSANSPKSLD
QYQSKIKNII NNQFNFIDFA NNQIYSKSNY LYQRSVVIAN NSNDLVNKIL NKKHIQTKNP
IISNMSFKGK NPITIFVFSG QGSQYPKMAL ELYNNEVIFK KSIDLIDSKL SKYYGYSVLE
KLRSIGDDDT TSIHDPTIAQ PAMCMFSVSL FELYKHWGVN PSFILGHSLG EIPTSYCSGM
IDLDTFCYTV YQRSIAQTKT HGNGRMLSIN ISDEEFKSMY SQKYPQIEIA CYNSPQSIVV
AGNESILNEI SKELKEKEIF TAMLGSLSSF HTSSQQCTKD SILQLNIESK KSKVPTFSTV
TTNLFNESDP FNSQYVYDNI INPVKFTQTI SNIYKHIESN QLDNDIVFIE IAPHPTLSFY
IKQMVPSSLN ESVSVYSALH KKKNDVEEFQ QTISNLYCQN GYNINFKCQF NNKKSNQKIN
LPLYQWDDEL YFAQTQVLEQ HRKEGPPIDH LGLSNSYYSP FKNSYRTFID IKNKPFQYLK
GHMVKGKYYF PGCGYIDNII QLYKNQDIFI SFIEFKTPLI LIEGINQCLQ TNIQQTGKSE
YRAQFHFKDQ KSNEWIQSSN ANFQLLDHTI EMPPKYNIEE IIKNKCTLAR LTKNEIYTHI
KSKTGLNYTA MFQGATECYI GVDCTLSVVS IESQTNSFLN IPILDSVFHG NISLINDQCQ
IVFDKVHGLK YYSSNIPNDY KDKSVYVYAK LKSKTSDSYS ASFTVLLSDG TVVYEIDEAF
SKSLIPIKEL LKIEYPNDEL FSINLQPKDS PIPAPSTFKS LIYENDRFNA EPPMVENLFQ
YISTIFYKNI INRCPEINID IIKSLSIDEI ISNFSKISKH ERLFKYVFET IKENGILNSL
EEKDDTYFVF NEVLIKSSRV ISKLLFPLEN DNDNEDTPQL LFQNGLMDKV YKCRYLKNKN
QMIAHIIKHS IKEIINNNII IRILEFGGGT ASLSVEVIEE IVTLLQENPN YQVEIEYTWS
DISPAFIVDT KNKINKIIKG AGITNGLNVI YHPLTIDESL VELQSIKPSY YDFVIMSNVL
HVVKDIKQAV EQMYQLLIPN GQLLFVEPPY KSIICDSLFG SFEQWWAFTD TDIRKDHCCL
SQDGWYKLLK WSNFESIEMS TESKFMGSVI QAQKPSFTSL INQQPKYDNI IIFGNNCPNF
IDNIKPFSNF NEFIQIETIQ EFDQLINKST ITNDSIIYFI KSINQLSLDN YKQATLEYIE
INQKLLQINS LCKHVLIVND SRKTNYLASS IVGAARYFDE FQQLKLHTLD FDYDSTQNYL
ISKNNKMVQF INNLIDSKTN VHKEMIIINN KVYYEIVQKE KNLKLKYNSE SFEQQDNLMC
SLSPNLEYQL QSKQIKLRDN QVEVKTIATG INNKDYLVYS GLEGSVNSNT PQFGYEFSGI
ITRVGNNVKD YKVGDNVFGL SNSCTSSNII VNHERIQIKP SNISHIEAAS IPIDYLTSFM
SLFNVGCLNI EDNESILIHL GSDSFGLSTF EILKWKGFKS NLFVTVNSDE IKQYLLNNYG
DLITAIYSNT DKGYVSQIKN KLIEQGSNDN GVDLILNTLP SDFMDSNFQL LAINGRIIDL
SNDHLNQSEY MKNVNFTLNR GYHNFDLMSQ RNSRINRSLL TISKAIENGE LKLIPIKEFS
NSDINDAIEF IIEENRIDKI VVSHDHEVYQ ELYAKFSNEN DFSILKSNYQ INSNNLGKNI
LITGQSGIIL EILKWIIKYS NINTIENVII LSRSSLKWEL ELLINQTKLS NNNIKFHFKS
VDVGDSEQVD NAINEILNEN QEIINIHSIF HFAFTQIACK VQEINMKHLD ISHGAKTMGA
INLHNQSIKR NWKLINFVIS SSIASLVGST DQCSYVCANA LLDSFSKYRV SLGLPSTSIN
LGAIESTGFV SKNESISVFL DGSGIIPTPI NQVLGLLDLQ IQNPGKFTNS MVAKFNPLNF
SNNEQINLLL KMDYIFNLHS NGYTKVKESA GSKNVDELFI KKISDLFSID ESKINKDIRL
IDYGADSLVI VQLKNWVDQE IGFNLITIQQ LQNNTINVSI KIILNFLKKI NK