PKSC_ARATH
ID PKSC_ARATH Reviewed; 385 AA.
AC O81305; Q8LD79;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Type III polyketide synthase C;
DE Short=PKS-C;
DE EC=2.3.1.-;
GN OrderedLocusNames=At4g00040 {ECO:0000312|Araport:AT4G00040};
GN ORFNames=F6N15.12 {ECO:0000312|EMBL:AAC19299.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=20442277; DOI=10.1104/pp.110.157446;
RA Dobritsa A.A., Lei Z., Nishikawa S., Urbanczyk-Wochniak E., Huhman D.V.,
RA Preuss D., Sumner L.W.;
RT "LAP5 and LAP6 encode anther-specific proteins with similarity to chalcone
RT synthase essential for pollen exine development in Arabidopsis.";
RL Plant Physiol. 153:937-955(2010).
CC -!- FUNCTION: Plant type III polyketide synthases (PKSs) that catalyzes the
CC condensation of malonyl-CoA units with various CoA ester starter
CC molecules to generate a diverse array of natural products including
CC long-chain alkyl alpha-pyrones. {ECO:0000250|UniProtKB:O23674}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000250|UniProtKB:Q9LKP7}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P30074}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:O23674}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AF069299; AAC19299.1; -; Genomic_DNA.
DR EMBL; AL161471; CAB80762.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE81817.1; -; Genomic_DNA.
DR EMBL; BT001993; AAN72004.1; -; mRNA.
DR EMBL; BT008463; AAP37822.1; -; mRNA.
DR EMBL; AY086158; AAM63363.1; -; mRNA.
DR PIR; T01332; T01332.
DR RefSeq; NP_191915.1; NM_116221.1.
DR AlphaFoldDB; O81305; -.
DR SMR; O81305; -.
DR STRING; 3702.AT4G00040.1; -.
DR PaxDb; O81305; -.
DR PRIDE; O81305; -.
DR EnsemblPlants; AT4G00040.1; AT4G00040.1; AT4G00040.
DR GeneID; 828224; -.
DR Gramene; AT4G00040.1; AT4G00040.1; AT4G00040.
DR KEGG; ath:AT4G00040; -.
DR Araport; AT4G00040; -.
DR TAIR; locus:2126861; AT4G00040.
DR eggNOG; ENOG502QSSY; Eukaryota.
DR HOGENOM; CLU_034992_2_0_1; -.
DR InParanoid; O81305; -.
DR OMA; GPAFCSE; -.
DR OrthoDB; 950070at2759; -.
DR PhylomeDB; O81305; -.
DR BioCyc; ARA:AT4G00040-MON; -.
DR UniPathway; UPA00154; -.
DR PRO; PR:O81305; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O81305; baseline and differential.
DR Genevisible; O81305; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0030639; P:polyketide biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
PE 2: Evidence at transcript level;
KW Acyltransferase; Developmental protein; Endoplasmic reticulum;
KW Flavonoid biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..385
FT /note="Type III polyketide synthase C"
FT /id="PRO_0000432842"
FT ACT_SITE 165
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q94FV7,
FT ECO:0000255|PROSITE-ProRule:PRU10023"
FT BINDING 56..63
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:P30074"
FT BINDING 217..218
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P30074"
FT BINDING 267
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:Q58VP7"
FT BINDING 307..310
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:Q58VP7"
FT BINDING 310
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:P30074"
FT CONFLICT 50
FT /note="N -> D (in Ref. 4; AAM63363)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="N -> K (in Ref. 4; AAM63363)"
FT /evidence="ECO:0000305"
FT CONFLICT 209
FT /note="N -> D (in Ref. 4; AAM63363)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 385 AA; 42415 MW; 4EC4C17A296819AB CRC64;
MLVSARVEKQ KRVAYQGKAT VLALGKALPS NVVSQENLVE EYLREIKCDN LSIKDKLQHL
CKSTTVKTRY TVMSRETLHK YPELATEGSP TIKQRLEIAN DAVVQMAYEA SLVCIKEWGR
AVEDITHLVY VSSSEFRLPG GDLYLSAQLG LSNEVQRVML YFLGCYGGLS GLRVAKDIAE
NNPGSRVLLT TSETTVLGFR PPNKARPYNL VGAALFGDGA AALIIGADPT ESESPFMELH
CAMQQFLPQT QGVIDGRLSE EGITFKLGRD LPQKIEDNVE EFCKKLVAKA GSGALELNDL
FWAVHPGGPA ILSGLETKLK LKPEKLECSR RALMDYGNVS SNTIFYIMDK VRDELEKKGT
EGEEWGLGLA FGPGITFEGF LMRNL
