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PKSH_BACSU
ID   PKSH_BACSU              Reviewed;         259 AA.
AC   P40805;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Probable polyketide biosynthesis enoyl-CoA hydratase PksH;
DE            EC=4.2.1.-;
GN   Name=pksH; OrderedLocusNames=BSU17160;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / PB1424;
RX   PubMed=7704258; DOI=10.1099/13500872-141-2-299;
RA   Albertini A.M., Caramori T., Scoffone F., Scotti C., Galizzi A.;
RT   "Sequence around the 159 degree region of the Bacillus subtilis genome: the
RT   pksX locus spans 33.6 kb.";
RL   Microbiology 141:299-309(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=16757561; DOI=10.1073/pnas.0603148103;
RA   Calderone C.T., Kowtoniuk W.E., Kelleher N.L., Walsh C.T., Dorrestein P.C.;
RT   "Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-
RT   S-carrier proteins in the pksX pathway of Bacillus subtilis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:8977-8982(2006).
RN   [4]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=17190806; DOI=10.1073/pnas.0609073103;
RA   Straight P.D., Fischbach M.A., Walsh C.T., Rudner D.Z., Kolter R.;
RT   "A singular enzymatic megacomplex from Bacillus subtilis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:305-310(2007).
RN   [5]
RP   FUNCTION IN BACILLAENE BIOSYNTHESIS.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=17234808; DOI=10.1073/pnas.0610503104;
RA   Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R.,
RA   Walsh C.T., Clardy J.;
RT   "The identification of bacillaene, the product of the PksX megacomplex in
RT   Bacillus subtilis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 3-259.
RG   New York structural genomics research consortium (NYSGRC);
RT   "Crystal structure of a putative polyketide biosynthesis enoyl-COA
RT   hydratase (pksH) from Bacillus subtilis.";
RL   Submitted (JUN-2009) to the PDB data bank.
CC   -!- FUNCTION: Involved in some intermediate steps for the synthesis of the
CC       antibiotic polyketide bacillaene which is involved in secondary
CC       metabolism. Probably catalyzes the dehydration of the (S)-3-hydroxy-3-
CC       methylglutaryl group attached to PksL. {ECO:0000269|PubMed:16757561,
CC       ECO:0000269|PubMed:17234808}.
CC   -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17190806}.
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; U11039; AAA85141.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13587.1; -; Genomic_DNA.
DR   PIR; F69678; F69678.
DR   RefSeq; NP_389596.1; NC_000964.3.
DR   RefSeq; WP_010886512.1; NZ_JNCM01000035.1.
DR   PDB; 3HP0; X-ray; 2.32 A; A/B/C/D/E/F=3-259.
DR   PDBsum; 3HP0; -.
DR   AlphaFoldDB; P40805; -.
DR   SMR; P40805; -.
DR   STRING; 224308.BSU17160; -.
DR   PaxDb; P40805; -.
DR   PRIDE; P40805; -.
DR   EnsemblBacteria; CAB13587; CAB13587; BSU_17160.
DR   GeneID; 940033; -.
DR   KEGG; bsu:BSU17160; -.
DR   PATRIC; fig|224308.43.peg.1812; -.
DR   eggNOG; COG1024; Bacteria.
DR   InParanoid; P40805; -.
DR   OMA; MTLMTKP; -.
DR   PhylomeDB; P40805; -.
DR   BioCyc; BSUB:BSU17160-MON; -.
DR   UniPathway; UPA01003; -.
DR   EvolutionaryTrace; P40805; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic biosynthesis; Cytoplasm; Lyase;
KW   Reference proteome.
FT   CHAIN           1..259
FT                   /note="Probable polyketide biosynthesis enoyl-CoA hydratase
FT                   PksH"
FT                   /id="PRO_0000109346"
FT   STRAND          7..14
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          17..22
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           25..27
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           34..48
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          53..57
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           69..74
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   TURN            75..79
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           87..98
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          99..107
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          109..112
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           115..121
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          122..127
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          132..134
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           137..140
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   TURN            145..147
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           148..155
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           157..166
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           172..178
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   STRAND          180..182
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           190..199
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           204..217
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           220..235
FT                   /evidence="ECO:0007829|PDB:3HP0"
FT   HELIX           241..248
FT                   /evidence="ECO:0007829|PDB:3HP0"
SQ   SEQUENCE   259 AA;  29411 MW;  441F76B77234DA9D CRC64;
     MDLVTYQTIK VRFQASVCYI TFHRPEANNT INDTLIEECL QVLNQCETST VTVVVLEGLP
     EVFCFGADFQ EIYQEMKRGR KQASSQEPLY DLWMKLQTGP YVTISHVRGK VNAGGLGFVS
     ATDIAIADQT ASFSLSELLF GLYPACVLPF LIRRIGRQKA HYMTLMTKPI SVQEASEWGL
     IDAFDAESDV LLRKHLLRLR RLNKKGIAHY KQFMSSLDHQ VSRAKATALT ANQDMFSDPQ
     NQMGIIRYVE TGQFPWEDQ
 
 
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