PKSI_BACSU
ID PKSI_BACSU Reviewed; 249 AA.
AC P40802;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Putative polyketide biosynthesis enoyl-CoA isomerase PksI;
DE EC=4.-.-.-;
GN Name=pksI; OrderedLocusNames=BSU17170;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / PB1424;
RX PubMed=7704258; DOI=10.1099/13500872-141-2-299;
RA Albertini A.M., Caramori T., Scoffone F., Scotti C., Galizzi A.;
RT "Sequence around the 159 degree region of the Bacillus subtilis genome: the
RT pksX locus spans 33.6 kb.";
RL Microbiology 141:299-309(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 189.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP FUNCTION.
RX PubMed=16757561; DOI=10.1073/pnas.0603148103;
RA Calderone C.T., Kowtoniuk W.E., Kelleher N.L., Walsh C.T., Dorrestein P.C.;
RT "Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-
RT S-carrier proteins in the pksX pathway of Bacillus subtilis.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:8977-8982(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=17190806; DOI=10.1073/pnas.0609073103;
RA Straight P.D., Fischbach M.A., Walsh C.T., Rudner D.Z., Kolter R.;
RT "A singular enzymatic megacomplex from Bacillus subtilis.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:305-310(2007).
RN [6]
RP FUNCTION IN BACILLAENE BIOSYNTHESIS.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=17234808; DOI=10.1073/pnas.0610503104;
RA Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R.,
RA Walsh C.T., Clardy J.;
RT "The identification of bacillaene, the product of the PksX megacomplex in
RT Bacillus subtilis.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007).
CC -!- FUNCTION: Involved in some intermediate steps for the synthesis of the
CC antibiotic polyketide bacillaene which is involved in secondary
CC metabolism. May have a role in the decarboxylation of the (S)-3-
CC methylglutaryl group attached to PksL. {ECO:0000269|PubMed:16757561,
CC ECO:0000269|PubMed:17234808}.
CC -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17190806}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
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DR EMBL; U11039; AAA85142.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13588.2; -; Genomic_DNA.
DR PIR; G69678; G69678.
DR RefSeq; NP_389597.2; NC_000964.3.
DR RefSeq; WP_003231800.1; NZ_JNCM01000035.1.
DR PDB; 4Q1G; X-ray; 2.10 A; A/B/C=1-249.
DR PDB; 4Q1H; X-ray; 1.93 A; A/B/C=1-249.
DR PDB; 4Q1I; X-ray; 2.10 A; A/B/C=1-249.
DR PDB; 4Q1J; X-ray; 2.17 A; A/B/C=1-249.
DR PDB; 4Q1K; X-ray; 1.75 A; A/B/C=1-249.
DR PDBsum; 4Q1G; -.
DR PDBsum; 4Q1H; -.
DR PDBsum; 4Q1I; -.
DR PDBsum; 4Q1J; -.
DR PDBsum; 4Q1K; -.
DR AlphaFoldDB; P40802; -.
DR SMR; P40802; -.
DR STRING; 224308.BSU17170; -.
DR PaxDb; P40802; -.
DR PRIDE; P40802; -.
DR EnsemblBacteria; CAB13588; CAB13588; BSU_17170.
DR GeneID; 940096; -.
DR KEGG; bsu:BSU17170; -.
DR PATRIC; fig|224308.179.peg.1862; -.
DR eggNOG; COG1024; Bacteria.
DR InParanoid; P40802; -.
DR OMA; MHEKTFH; -.
DR PhylomeDB; P40802; -.
DR BioCyc; BSUB:BSU17170-MON; -.
DR UniPathway; UPA01003; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IBA:GO_Central.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic biosynthesis; Cytoplasm; Lyase;
KW Reference proteome.
FT CHAIN 1..249
FT /note="Putative polyketide biosynthesis enoyl-CoA isomerase
FT PksI"
FT /id="PRO_0000109347"
FT CONFLICT 189
FT /note="A -> E (in Ref. 1; AAA85142)"
FT /evidence="ECO:0000305"
FT STRAND 4..12
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 15..20
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 23..25
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 31..46
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 61..63
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 68..75
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:4Q1H"
FT HELIX 86..88
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 89..92
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 108..114
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 116..122
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 131..134
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 142..150
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 152..161
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 167..173
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 176..180
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 182..196
FT /evidence="ECO:0007829|PDB:4Q1K"
FT HELIX 201..232
FT /evidence="ECO:0007829|PDB:4Q1K"
FT STRAND 234..236
FT /evidence="ECO:0007829|PDB:4Q1G"
FT HELIX 237..246
FT /evidence="ECO:0007829|PDB:4Q1K"
SQ SEQUENCE 249 AA; 27896 MW; FBBCDA0DCBD9C911 CRC64;
MTHSVVELIE IESAIIQVKM QDRTHKNAFS QELTDDLIQA FEYIRQNPKY KAVILTGYDN
YFASGGTQEG LLRIQQGLTK FTDDNLYSLA LDCEIPVIAA MQGHGIGGGF VMGLFADIVI
LSRESVYTAN FMKYGFTPGM GATFIVPKKL GFSLAQEILL NAGSYRGADL EKRGVPFKVL
PRAEVLDYAV ELAQELAEKP RNSLVTLKDH LVAPLRDQLP RVIEQELMMH EKTFHHEEVK
SRIKGLYGN
