PKSS_BACSU
ID PKSS_BACSU Reviewed; 405 AA.
AC O31785;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Polyketide biosynthesis cytochrome P450 PksS;
DE EC=1.14.-.-;
GN Name=pksS; OrderedLocusNames=BSU17230;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP SEQUENCE REVISION TO C-TERMINUS.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [3]
RP FUNCTION, AND SUBSTRATE SPECIFICITY.
RC STRAIN=168;
RX PubMed=17482575; DOI=10.1016/j.bbrc.2007.04.151;
RA Reddick J.J., Antolak S.A., Raner G.M.;
RT "PksS from Bacillus subtilis is a cytochrome P450 involved in bacillaene
RT metabolism.";
RL Biochem. Biophys. Res. Commun. 358:363-367(2007).
CC -!- FUNCTION: Involved in the metabolism of the antibiotic polyketide
CC bacillaene which is involved in secondary metabolism. The substrate is
CC dihydrobacillaene. {ECO:0000269|PubMed:17482575}.
CC -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AL009126; CAB13607.2; -; Genomic_DNA.
DR RefSeq; NP_389605.2; NC_000964.3.
DR RefSeq; WP_003244857.1; NZ_JNCM01000035.1.
DR PDB; 4YZR; X-ray; 1.35 A; A=1-405.
DR PDBsum; 4YZR; -.
DR AlphaFoldDB; O31785; -.
DR SMR; O31785; -.
DR STRING; 224308.BSU17230; -.
DR PaxDb; O31785; -.
DR PRIDE; O31785; -.
DR EnsemblBacteria; CAB13607; CAB13607; BSU_17230.
DR GeneID; 940046; -.
DR KEGG; bsu:BSU17230; -.
DR PATRIC; fig|224308.179.peg.1868; -.
DR eggNOG; COG2124; Bacteria.
DR InParanoid; O31785; -.
DR OMA; NCIFLLN; -.
DR PhylomeDB; O31785; -.
DR BioCyc; BSUB:BSU17230-MON; -.
DR UniPathway; UPA01003; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002397; Cyt_P450_B.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00359; BP450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic biosynthesis; Cell membrane; Heme; Iron; Membrane;
KW Metal-binding; Monooxygenase; Oxidoreductase; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..405
FT /note="Polyketide biosynthesis cytochrome P450 PksS"
FT /id="PRO_0000380708"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 352
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT HELIX 11..15
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 17..27
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 29..35
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 50..58
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 66..69
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 85..88
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 91..93
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 98..106
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 107..110
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 112..116
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 119..133
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 136..140
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 141..144
FT /evidence="ECO:0007829|PDB:4YZR"
FT TURN 145..147
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 148..158
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 162..164
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 165..175
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 180..182
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 183..206
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 212..218
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 228..259
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 261..269
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 271..273
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 274..285
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 295..298
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 300..302
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 305..307
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 312..315
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 317..320
FT /evidence="ECO:0007829|PDB:4YZR"
FT TURN 324..326
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 327..329
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 355..372
FT /evidence="ECO:0007829|PDB:4YZR"
FT HELIX 382..384
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 391..393
FT /evidence="ECO:0007829|PDB:4YZR"
FT STRAND 400..402
FT /evidence="ECO:0007829|PDB:4YZR"
SQ SEQUENCE 405 AA; 46729 MW; 99AD907CC96DD161 CRC64;
MEKLMFHPHG KEFHHNPFSV LGRFREEEPI HRFELKRFGA TYPAWLITRY DDCMAFLKDN
RITRDVKNVM NQEQIKMLNV SEDIDFVSDH MLAKDTPDHT RLRSLVHQAF TPRTIENLRG
SIEQIAEQLL DEMEKENKAD IMKSFASPLP FIVISELMGI PKEDRSQFQI WTNAMVDTSE
GNRELTNQAL REFKDYIAKL IHDRRIKPKD DLISKLVHAE ENGSKLSEKE LYSMLFLLVV
AGLETTVNLL GSGTLALLQH KKECEKLKQQ PEMIATAVEE LLRYTSPVVM MANRWAIEDF
TYKGHSIKRG DMIFIGIGSA NRDPNFFENP EILNINRSPN RHISFGFGIH FCLGAPLARL
EGHIAFKALL KRFPDIELAV APDDIQWRKN VFLRGLESLP VSLSK
