ID   PL1_ARATH               Reviewed;         334 AA.
AC   A0A1P8B9P9; Q9LEV1;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2017, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Protein POLAR-like 1 {ECO:0000303|PubMed:30429609};
GN   Name=PL1 {ECO:0000303|PubMed:30429609};
GN   OrderedLocusNames=At5g10890 {ECO:0000312|Araport:AT5G10890};
GN   ORFNames=T30N20.160 {ECO:0000312|EMBL:CAB96844.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Columbia;
RX   PubMed=30429609; DOI=10.1038/s41586-018-0714-x;
RA   Houbaert A., Zhang C., Tiwari M., Wang K., de Marcos Serrano A.,
RA   Savatin D.V., Urs M.J., Zhiponova M.K., Gudesblat G.E., Vanhoutte I.,
RA   Eeckhout D., Boeren S., Karimi M., Betti C., Jacobs T., Fenoll C., Mena M.,
RA   de Vries S., De Jaeger G., Russinova E.;
RT   "POLAR-guided signalling complex assembly and localization drive asymmetric
RT   cell division.";
RL   Nature 563:574-578(2018).
CC   -!- FUNCTION: Acts as a stomatal lineage scaffold which regulates
CC       subcellular localization and transient polarization of kinases (e.g.
CC       ASK7/BIN2 and ASK3/SK12) involved in asymmetric cell division (ACD) in
CC       a BASL-dependent manner. {ECO:0000269|PubMed:30429609}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6NQ99}.
CC       Cytoplasm, cell cortex {ECO:0000250|UniProtKB:Q6NQ99}. Note=Localized
CC       throughout the plasma membrane in asymmetric cell division (ACD)
CC       precursors (By similarity). Transient polar localization and
CC       segregation unevenly (distal to the newly divided meristemoid and
CC       parallel to the division plane) during meristemoid asymmetric divisions
CC       in a BASL-dependent manner (By similarity). Present in the cytoplasm of
CC       meristemoids and cell periphery in stomatal lineage ground cells
CC       (SLGCs), in a polarized pattern (By similarity).
CC       {ECO:0000250|UniProtKB:Q6NQ99}.
CC   -!- DISRUPTION PHENOTYPE: Decreased accumulation of ASK7/BIN2 at the plasma
CC       membrane and impaired polarization of ASK7/BIN2 in asymmetric cell
CC       division (ACD) precursors thus leading to its nuclear localization in
CC       the meristemoids. {ECO:0000269|PubMed:30429609}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AED91606.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CAB96844.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL365234; CAB96844.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; AED91606.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; ANM68326.1; -; Genomic_DNA.
DR   PIR; T50798; T50798.
DR   RefSeq; NP_001330090.1; NM_001343140.1.
DR   RefSeq; NP_196650.1; NM_121127.2.
DR   AlphaFoldDB; A0A1P8B9P9; -.
DR   SMR; A0A1P8B9P9; -.
DR   EnsemblPlants; AT5G10890.2; AT5G10890.2; AT5G10890.
DR   GeneID; 830956; -.
DR   Gramene; AT5G10890.2; AT5G10890.2; AT5G10890.
DR   KEGG; ath:AT5G10890; -.
DR   Araport; AT5G10890; -.
DR   TAIR; locus:2183785; AT5G10890.
DR   eggNOG; ENOG502RZ5T; Eukaryota.
DR   HOGENOM; CLU_063347_1_0_1; -.
DR   OMA; NSKMELQ; -.
DR   OrthoDB; 1373738at2759; -.
DR   PRO; PR:A0A1P8B9P9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; A0A1P8B9P9; baseline and differential.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0008356; P:asymmetric cell division; IEA:InterPro.
DR   InterPro; IPR040348; POLAR-like.
DR   PANTHER; PTHR33476; PTHR33476; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Reference proteome.
FT   CHAIN           1..334
FT                   /note="Protein POLAR-like 1"
FT                   /id="PRO_0000446320"
FT   REGION          53..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          273..300
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   334 AA;  38571 MW;  5A9C284803E664DD CRC64;
     MDDGVGVRAG CIRIRCPSPR RIVSRWLSPQ GKVKGKKTPM AEPRVRDSDT VRIRTSSEDD
     HHRVGQFSDS PPPTIPSELQ RREFLFSIGM SCYLIHLIAT GRQEIHKIVE LRNDLDKFLE
     CRNEELRQKQ QEFVELRNDI HKFLEFHNNE LRRKQLEKTE TSAYSATSDV VDGPESSTDH
     YYSPQIIQTS MSVGGEGSLS HYVYKLENDS GGEMDQLEAE LEAEFELLQI GHNQEVSEDA
     EGLRLGHVCP GLVEEQQGVC PYELERRLYE LMETRQQEEI KELEIALDDA KQRLHLKETE
     ASWWKDTAYI VSERIPEPSR ITHSSRTHPY PLSR