PLA18_ARATH
ID PLA18_ARATH Reviewed; 423 AA.
AC Q9LNC2; Q8LF19;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Phospholipase A1-IIalpha;
DE EC=3.1.1.-;
GN OrderedLocusNames=At1g06250; ORFNames=F9P14.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15130548; DOI=10.1016/j.tplants.2004.03.004;
RA Ryu S.B.;
RT "Phospholipid-derived signaling mediated by phospholipase A in plants.";
RL Trends Plant Sci. 9:229-235(2004).
CC -!- FUNCTION: Acylhydrolase that catalyzes the hydrolysis of phospholipids
CC at the sn-1 position. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
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DR EMBL; AC025290; AAF80222.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27968.1; -; Genomic_DNA.
DR EMBL; AY085093; AAM61647.1; -; mRNA.
DR PIR; C86198; C86198.
DR RefSeq; NP_172115.1; NM_100507.2.
DR AlphaFoldDB; Q9LNC2; -.
DR SMR; Q9LNC2; -.
DR ESTHER; arath-F9P14.11; Plant_phospholipase.
DR iPTMnet; Q9LNC2; -.
DR PaxDb; Q9LNC2; -.
DR PRIDE; Q9LNC2; -.
DR ProteomicsDB; 226197; -.
DR EnsemblPlants; AT1G06250.1; AT1G06250.1; AT1G06250.
DR GeneID; 837135; -.
DR Gramene; AT1G06250.1; AT1G06250.1; AT1G06250.
DR KEGG; ath:AT1G06250; -.
DR Araport; AT1G06250; -.
DR TAIR; locus:2038505; AT1G06250.
DR eggNOG; KOG4569; Eukaryota.
DR HOGENOM; CLU_018841_0_0_1; -.
DR InParanoid; Q9LNC2; -.
DR OMA; EGIPKRW; -.
DR OrthoDB; 612344at2759; -.
DR PhylomeDB; Q9LNC2; -.
DR BioCyc; ARA:AT1G06250-MON; -.
DR PRO; PR:Q9LNC2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LNC2; baseline and differential.
DR Genevisible; Q9LNC2; AT.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0008970; F:phospholipase A1 activity; ISS:UniProtKB.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR InterPro; IPR033556; PLA.
DR PANTHER; PTHR31828; PTHR31828; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00120; LIPASE_SER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome.
FT CHAIN 1..423
FT /note="Phospholipase A1-IIalpha"
FT /id="PRO_0000409358"
FT REGION 399..423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 194..217
FT /evidence="ECO:0000255"
FT ACT_SITE 223
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 223
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
FT ACT_SITE 290
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
FT ACT_SITE 327
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
FT CONFLICT 67
FT /note="L -> F (in Ref. 3; AAM61647)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="R -> H (in Ref. 3; AAM61647)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 423 AA; 48123 MW; E410984DC9F6DC74 CRC64;
MVEGIPKRWK VLSGQNKWKG LLDPLDPDLR RYIIHYGEMS QVGYDAFNWD RKSRYAGDCY
YSKNRLLART GFLKANPFRY KVTKYIYATA SIKLPISFIV KSLSKDASRV QTNWMGYIAV
ATDQGKAMLG RRDIVVAWRG TLQPYEWAND FDFPLEPAIS VFPVTDPKDN PRIGSGWLDI
YTASDSRSPY DTTSAQEQVQ GELKRLLELY KDEEISITFT GHSLGAVMSV LSAADLVYGK
KNNININLQK KQVPITVFAF GSPRIGDHNF KNVVDSLQPL NILRIVNVPD VAPHYPLLLY
SEIGEVLEIN TLNSTYLKRS LNFRNYHNLE IYLHGMAGMQ DTDGVFKLEI GRDISLVNKG
LDALKDEYLV PSTWRCLANK GMLQMDDGTW KLDVHRRDHD DDVDADDNDD SSTSNQLQEL
NTD
