PLA2_CAEEL
ID PLA2_CAEEL Reviewed; 1071 AA.
AC Q20500;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Intracellular phospholipase A2;
DE Short=IPLA-2;
DE Short=PLA(2);
DE EC=3.1.1.4 {ECO:0000250|UniProtKB:P97570};
DE AltName: Full=Calcium-independent phospholipase A2 {ECO:0000303|PubMed:23007400};
GN Name=ipla-2 {ECO:0000312|EMBL:CAA90061.1, ECO:0000312|WormBase:F47A4.5};
GN ORFNames=F47A4.5 {ECO:0000312|WormBase:F47A4.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CAA90061.1,
RC ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23007400; DOI=10.1074/jbc.m112.391508;
RA Morrison K., Witte K., Mayers J.R., Schuh A.L., Audhya A.;
RT "Roles of acidic phospholipids and nucleotides in regulating membrane
RT binding and activity of a calcium-independent phospholipase A2 isoform.";
RL J. Biol. Chem. 287:38824-38834(2012).
CC -!- FUNCTION: Phospholipase that plays a critical role during oogenesis,
CC ovulation, and/or embryogenesis. {ECO:0000269|PubMed:23007400}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-
CC glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4;
CC Evidence={ECO:0000250|UniProtKB:P97570};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15802;
CC Evidence={ECO:0000250|UniProtKB:P97570};
CC -!- DISRUPTION PHENOTYPE: Mutant animals generate fewer progeny and slower
CC larval development. {ECO:0000269|PubMed:23007400}.
CC -!- SIMILARITY: Belongs to the patatin family. {ECO:0000305}.
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DR EMBL; BX284606; CAA90061.1; -; Genomic_DNA.
DR PIR; T22327; T22327.
DR RefSeq; NP_509647.1; NM_077246.4.
DR AlphaFoldDB; Q20500; -.
DR SMR; Q20500; -.
DR STRING; 6239.F47A4.5; -.
DR EPD; Q20500; -.
DR PaxDb; Q20500; -.
DR PeptideAtlas; Q20500; -.
DR EnsemblMetazoa; F47A4.5.1; F47A4.5.1; WBGene00009801.
DR GeneID; 181196; -.
DR KEGG; cel:CELE_F47A4.5; -.
DR UCSC; F47A4.5; c. elegans.
DR CTD; 181196; -.
DR WormBase; F47A4.5; CE02248; WBGene00009801; ipla-2.
DR eggNOG; KOG0513; Eukaryota.
DR HOGENOM; CLU_287465_0_0_1; -.
DR InParanoid; Q20500; -.
DR OMA; CHTENCY; -.
DR OrthoDB; 146995at2759; -.
DR PhylomeDB; Q20500; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00009801; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0004623; F:phospholipase A2 activity; IEA:UniProtKB-EC.
DR GO; GO:0051017; P:actin filament bundle assembly; IBA:GO_Central.
DR GO; GO:0044258; P:intestinal lipid catabolic process; IMP:WormBase.
DR GO; GO:0042594; P:response to starvation; IEP:WormBase.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR002641; PNPLA_dom.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF01734; Patatin; 1.
DR SMART; SM00248; ANK; 6.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
DR PROSITE; PS51635; PNPLA; 1.
PE 3: Inferred from homology;
KW ANK repeat; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Repeat.
FT CHAIN 1..1071
FT /note="Intracellular phospholipase A2"
FT /id="PRO_0000452652"
FT REPEAT 411..440
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 479..508
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REPEAT 510..539
FT /note="ANK 4"
FT /evidence="ECO:0000255"
FT REPEAT 544..570
FT /note="ANK 5"
FT /evidence="ECO:0000255"
FT REPEAT 578..610
FT /note="ANK 6"
FT /evidence="ECO:0000255"
FT REPEAT 614..651
FT /note="ANK 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00023"
FT REPEAT 652..681
FT /note="ANK 7"
FT /evidence="ECO:0000255"
FT DOMAIN 748..921
FT /note="PNPLA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 752..757
FT /note="GXGXXG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 784..788
FT /note="GXSXG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 908..910
FT /note="DGA/G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT ACT_SITE 786
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT ACT_SITE 908
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
SQ SEQUENCE 1071 AA; 119090 MW; 138067CA3E31C1D5 CRC64;
MTTTNKDGPF RQQYLPGVHK EPNLAISEST HSSDWYSATS SEDCFQAASQ SFSSQKSNGN
VITQQPGKLH CEISDPKSCS FVPAISTDNA QMGTSGIIKY RSYSNPDLLV AMSPLAYRDE
APQSQKSVAN SRQDLTQTSC EVVTVSETVV NADSITTTTV TSSTTTTATA SQEDFIMVEA
EQDETAGLCD ATMPLVDKKN ITTSSTTSSP SRQITPRIEN GLSFENVVSL VKIFPAFVNK
LLKKPWYASR CDEVCQFPKA ELAYYDIVST DVDDEHFVIV AGKVTTDEHL MDNQFHLVFA
PVGFSEDVSL DERPFSLFRA TDKKDLMDLL HLCDEKSFLF TSLDMSTMRA DILRSKIEEL
VIQIRLKPHY HMIHVAIATD RLDFFSDGMI KTMNETLEPF ESQLRCLCHT ENCYPVHLAL
TMDRQKIVER LLELDPTLFC ETDKAGNNVW HHVNSSFCAQ IIWDRCPASQ HFIDERNMDG
QSPLNEAVST AKPLVATFLI GKGAKFTRGD RNELFVAMTS KNAQSVVEVV LTDKPEIANE
RDALGNSAIH VALYKESLNA LLNRKVELGL DIDVKNNAGE TALLLFITTR KPDLLPLLVT
LYAHGANMNA TDPHGNTALH KSAALVDAKK ISLECVKFLI SAGSNPNKIN LRGESPRHLA
ASLQNQEMLA ILKAAGATRC PKGYKGCRSN CRHDCSSAED EYEETLQKIR IGNESDYEKT
EFTASEKLNI QDTLDGSRRG KKAKVNLISM DGGGIRGLVI IQTLIAIEER LGDDIFKYFD
WSAGTSTGSL IMAGLATGKS LREMQQTYLL LKDRVFDGIM PPYDTVQLEK FIQDQFGTGT
VWEIPYPRLM ISAVNSEKLP VRLEMARNYK PAKDVAPETP KEMPLWMALR RSTAAPVLFK
PSEDRYIDGG IISNNPALDL MSEVHAYNRE LQLSGRKSDA VQMNVLVSFG TGQIPSTVIE
TLSIDSNSPL QSIKTIKNLA AMFIDQATAS EGAPVARSRQ WADSLEIPFF RFSAPLSKNI
FLSSTSDLDV CTMMWDSFIY CRKHRDYIDE LVKVLKHDTD HPHVKTPFTD L
