ID   PLAP_KLEPN              Reviewed;         197 AA.
AC   P37103;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Probable low-affinity putrescine importer PlaP {ECO:0000250|UniProtKB:P0AA47};
DE   Flags: Fragment;
GN   Name=plaP;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3062178; DOI=10.1016/0022-2836(88)90205-7;
RA   Arnold W., Rump A., Klipp W., Priefer U.B., Puehler A.;
RT   "Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire
RT   nitrogen fixation gene cluster of Klebsiella pneumoniae.";
RL   J. Mol. Biol. 203:715-738(1988).
RN   [2]
RP   IDENTIFICATION.
RA   Robison K.;
RL   Unpublished observations (JUL-1994).
CC   -!- FUNCTION: Putrescine importer. {ECO:0000250|UniProtKB:P0AA47}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(in) + putrescine(in) = H(+)(out) + putrescine(out);
CC         Xref=Rhea:RHEA:28891, ChEBI:CHEBI:15378, ChEBI:CHEBI:326268;
CC         Evidence={ECO:0000250|UniProtKB:P0AA47};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28893;
CC         Evidence={ECO:0000250|UniProtKB:P0AA47};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0AA47}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=X13303; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X13303; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR002293; AA/rel_permease1.
DR   Pfam; PF13520; AA_permease_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           <1..197
FT                   /note="Probable low-affinity putrescine importer PlaP"
FT                   /id="PRO_0000054217"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   197 AA;  21819 MW;  9348195B04D6EF6D CRC64;
     LQLYFPDISR FKDPDASQPE IMLYVAGKTF QWGVLIFSSV TVLASGTAAH AGVSRLMYVM
     GRDGVFPTRF FGYVHPKRRT PAWNVLLVXA IALLAIKLDL VTATAPINLG ALVAFTFVNL
     SVISQFWIRE KRNKTLKDHF NYLILPVCGA LTVGALWINL EESSMVLGLI WGGIGLVYXA
     CVTKSFRNPV PQYEDVA