PLAS1_ARATH
ID PLAS1_ARATH Reviewed; 171 AA.
AC P11490; P93656; Q52K91; Q9SGS3;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Plastocyanin minor isoform, chloroplastic;
DE Flags: Precursor;
GN Name=PETE; OrderedLocusNames=At1g76100; ORFNames=T23E18.3, T23E18_39;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3396882; DOI=10.1016/0378-1119(88)90417-9;
RA Vorst O., Oosterhoff-Teertstra R., Vankan P., Smeekens S., Weisbeek P.J.;
RT "Plastocyanin of Arabidopsis thaliana; isolation and characterization of
RT the gene and chloroplast import of the precursor protein.";
RL Gene 65:59-69(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9380515; DOI=10.1093/nar/25.19.3904;
RA van Drunen C.M., Oosterling R.W., Keultjes G.M., Weisbeek P.J.,
RA van Driel R., Smeekens S.C.M.;
RT "Analysis of the chromatin domain organisation around the plastocyanin gene
RT reveals an MAR-specific sequence element in Arabidopsis thaliana.";
RL Nucleic Acids Res. 25:3904-3911(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP PROTEIN SEQUENCE OF 73-80, AND SUBCELLULAR LOCATION.
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
CC -!- FUNCTION: Participates in electron transfer between P700 and the
CC cytochrome b6-f complex in photosystem I. Seems to be a minor
CC plastocyanin in Arabidopsis.
CC -!- COFACTOR:
CC Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC Evidence={ECO:0000250|UniProtKB:P18068};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:11719511}; Peripheral membrane protein
CC {ECO:0000269|PubMed:11719511}; Lumenal side
CC {ECO:0000269|PubMed:11719511}. Note=Loosely bound to the inner
CC thylakoid membrane surface in chloroplasts.
CC -!- SIMILARITY: Belongs to the plastocyanin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF17650.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M20937; AAA32834.1; -; Genomic_DNA.
DR EMBL; Z83321; CAB05911.1; -; Genomic_DNA.
DR EMBL; AC009978; AAF17650.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE35796.1; -; Genomic_DNA.
DR EMBL; BT021977; AAY17414.1; -; mRNA.
DR EMBL; BT025525; ABF58943.1; -; mRNA.
DR EMBL; AK226194; BAE98359.1; -; mRNA.
DR PIR; JA0065; CUMUM.
DR RefSeq; NP_001321069.1; NM_001334709.1.
DR RefSeq; NP_177737.1; NM_106259.4.
DR AlphaFoldDB; P11490; -.
DR SMR; P11490; -.
DR STRING; 3702.AT1G76100.1; -.
DR PaxDb; P11490; -.
DR PRIDE; P11490; -.
DR EnsemblPlants; AT1G76100.1; AT1G76100.1; AT1G76100.
DR GeneID; 843942; -.
DR Gramene; AT1G76100.1; AT1G76100.1; AT1G76100.
DR KEGG; ath:AT1G76100; -.
DR Araport; AT1G76100; -.
DR TAIR; locus:2199787; AT1G76100.
DR eggNOG; ENOG502RXIY; Eukaryota.
DR HOGENOM; CLU_084115_0_0_1; -.
DR InParanoid; P11490; -.
DR OrthoDB; 1543670at2759; -.
DR PhylomeDB; P11490; -.
DR PRO; PR:P11490; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P11490; baseline and differential.
DR Genevisible; P11490; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR CDD; cd04219; Plastocyanin; 1.
DR Gene3D; 2.60.40.420; -; 1.
DR InterPro; IPR000923; BlueCu_1.
DR InterPro; IPR028871; BlueCu_1_BS.
DR InterPro; IPR001235; Copper_blue_Plastocyanin.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR002387; Plastocyanin.
DR Pfam; PF00127; Copper-bind; 1.
DR PRINTS; PR00156; COPPERBLUE.
DR PRINTS; PR00157; PLASTOCYANIN.
DR SUPFAM; SSF49503; SSF49503; 1.
DR TIGRFAMs; TIGR02656; cyanin_plasto; 1.
DR PROSITE; PS00196; COPPER_BLUE; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Copper; Direct protein sequencing; Electron transport;
KW Membrane; Metal-binding; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Transport.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT ?..72
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:11719511"
FT CHAIN 73..171
FT /note="Plastocyanin minor isoform, chloroplastic"
FT /id="PRO_0000002884"
FT DOMAIN 73..171
FT /note="Plastocyanin-like"
FT BINDING 109
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P18068"
FT BINDING 156
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P18068"
FT BINDING 159
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P18068"
FT BINDING 164
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P18068"
FT CONFLICT 35
FT /note="T -> S (in Ref. 1; AAA32834)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 171 AA; 17589 MW; F74CD9807ED626B2 CRC64;
MAAITSATVT IPSFTGLKLA VSSKPKTLST ISRSTSATRA PPKLALKSSL KDFGVIAVAT
AASIVLAGNA MAMEVLLGSD DGSLAFVPSE FTVAKGEKIV FKNNAGFPHN VVFDEDEIPS
GVDASKISMD ETALLNGAGE TYEVTLTEPG SYGFYCAPHQ GAGMVGKLTV K
