ID   PLAS2_TOBAC             Reviewed;          99 AA.
AC   P35477;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Plastocyanin B'/B'';
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Virginia; TISSUE=Leaf;
RX   PubMed=8268198; DOI=10.1016/0167-4838(93)90081-2;
RA   Dimitrov M.I., Donchev A.A., Egorov T.A.;
RT   "Twin plastocyanin dimorphism in tobacco.";
RL   Biochim. Biophys. Acta 1203:184-190(1993).
CC   -!- FUNCTION: Participates in electron transfer between P700 and the
CC       cytochrome b6-f complex in photosystem I.
CC       {ECO:0000250|UniProtKB:P18068}.
CC   -!- COFACTOR:
CC       Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC         Evidence={ECO:0000250|UniProtKB:P18068};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000269|PubMed:8268198}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:P18068}; Lumenal side
CC       {ECO:0000250|UniProtKB:P18068}. Note=Loosely bound to the inner
CC       thylakoid membrane surface in chloroplasts and/or in the lumen (By
CC       similarity).
CC   -!- MISCELLANEOUS: The sequence shown is that of plastocyanin B'.
CC   -!- SIMILARITY: Belongs to the plastocyanin family. {ECO:0000305}.
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DR   PIR; S40487; S40487.
DR   PIR; S40488; S40488.
DR   AlphaFoldDB; P35477; -.
DR   SMR; P35477; -.
DR   STRING; 4097.P35477; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   CDD; cd04219; Plastocyanin; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR028871; BlueCu_1_BS.
DR   InterPro; IPR001235; Copper_blue_Plastocyanin.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR002387; Plastocyanin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   PRINTS; PR00156; COPPERBLUE.
DR   PRINTS; PR00157; PLASTOCYANIN.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR02656; cyanin_plasto; 1.
DR   PROSITE; PS00196; COPPER_BLUE; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Copper; Direct protein sequencing; Electron transport;
KW   Membrane; Metal-binding; Plastid; Reference proteome; Thylakoid; Transport.
FT   CHAIN           1..99
FT                   /note="Plastocyanin B'/B''"
FT                   /id="PRO_0000085577"
FT   DOMAIN          1..99
FT                   /note="Plastocyanin-like"
FT   BINDING         37
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250|UniProtKB:P18068"
FT   BINDING         84
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250|UniProtKB:P18068"
FT   BINDING         87
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250|UniProtKB:P18068"
FT   BINDING         92
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250|UniProtKB:P18068"
FT   VARIANT         65
FT                   /note="G -> A (in plastocyanin B'')"
FT   VARIANT         81
FT                   /note="T -> S (in plastocyanin B'')"
FT   VARIANT         85
FT                   /note="A -> S (in plastocyanin B'')"
SQ   SEQUENCE   99 AA;  10358 MW;  3101896E0035E1FB CRC64;
     IEVLLGSDDG GLAFVPGNFS VSAGEKITFK NNAGFPHNVV FDEDEIPAGV DASKISMSEE
     DLLNGPGETY SVTLSEKGTY TFYCAPHQGA GMVGKVTVN