PLAS_SYNY3
ID PLAS_SYNY3 Reviewed; 126 AA.
AC P21697;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Plastocyanin;
DE Flags: Precursor;
GN Name=petE; OrderedLocusNames=sll0199;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION BY COPPER.
RX PubMed=2129338; DOI=10.1007/bf00017837;
RA Briggs L.M., Pecoraro V.L., McIntosh L.;
RT "Copper-induced expression, cloning, and regulatory studies of the
RT plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803.";
RL Plant Mol. Biol. 15:633-642(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [4]
RP PROTEIN SEQUENCE OF 29-47.
RX PubMed=9298645; DOI=10.1002/elps.1150180806;
RA Sazuka T., Ohara O.;
RT "Towards a proteome project of cyanobacterium Synechocystis sp. strain
RT PCC6803: linking 130 protein spots with their respective genes.";
RL Electrophoresis 18:1252-1258(1997).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 29-126 IN COMPLEX WITH COPPER,
RP FUNCTION, COFACTOR, AND SUBCELLULAR LOCATION.
RX PubMed=9466912; DOI=10.1006/jmbi.1997.1455;
RA Romero A., de la Cerda B., Varela P.F., Navarro J.A., Hervas M.,
RA de la Rosa M.A.;
RT "The 2.15 A crystal structure of a triple mutant plastocyanin from the
RT cyanobacterium Synechocystis sp. PCC 6803.";
RL J. Mol. Biol. 275:327-336(1998).
CC -!- FUNCTION: Participates in electron transfer between P700 and the
CC cytochrome b6-f complex in photosystem I. {ECO:0000255|HAMAP-
CC Rule:MF_00566, ECO:0000269|PubMed:9466912}.
CC -!- COFACTOR:
CC Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00566,
CC ECO:0000269|PubMed:9466912};
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:9466912}; Peripheral membrane protein
CC {ECO:0000269|PubMed:9466912}; Lumenal side
CC {ECO:0000269|PubMed:9466912}. Note=Loosely bound to the thylakoid inner
CC membrane surface (PubMed:9466912).
CC -!- INDUCTION: By copper. {ECO:0000269|PubMed:2129338}.
CC -!- SIMILARITY: Belongs to the plastocyanin family. {ECO:0000255|HAMAP-
CC Rule:MF_00566}.
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DR EMBL; X54105; CAA38038.1; -; Genomic_DNA.
DR EMBL; BA000022; BAA10227.1; -; Genomic_DNA.
DR PIR; S76375; S76375.
DR PDB; 1J5C; NMR; -; A=29-126.
DR PDB; 1J5D; NMR; -; A=29-126.
DR PDB; 1JXD; NMR; -; A=29-126.
DR PDB; 1JXF; NMR; -; A=29-126.
DR PDB; 1M9W; NMR; -; A=29-126.
DR PDB; 1PCS; X-ray; 2.15 A; A=29-126.
DR PDBsum; 1J5C; -.
DR PDBsum; 1J5D; -.
DR PDBsum; 1JXD; -.
DR PDBsum; 1JXF; -.
DR PDBsum; 1M9W; -.
DR PDBsum; 1PCS; -.
DR AlphaFoldDB; P21697; -.
DR BMRB; P21697; -.
DR SMR; P21697; -.
DR IntAct; P21697; 2.
DR STRING; 1148.1001599; -.
DR PaxDb; P21697; -.
DR EnsemblBacteria; BAA10227; BAA10227; BAA10227.
DR KEGG; syn:sll0199; -.
DR eggNOG; COG3794; Bacteria.
DR InParanoid; P21697; -.
DR OMA; YDYYCEP; -.
DR PhylomeDB; P21697; -.
DR EvolutionaryTrace; P21697; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005507; F:copper ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR CDD; cd04219; Plastocyanin; 1.
DR Gene3D; 2.60.40.420; -; 1.
DR HAMAP; MF_00566; Cytb6_f_plastocyanin; 1.
DR InterPro; IPR000923; BlueCu_1.
DR InterPro; IPR028871; BlueCu_1_BS.
DR InterPro; IPR001235; Copper_blue_Plastocyanin.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR002387; Plastocyanin.
DR InterPro; IPR023511; Plastocyanin_cyanobac.
DR Pfam; PF00127; Copper-bind; 1.
DR PRINTS; PR00156; COPPERBLUE.
DR PRINTS; PR00157; PLASTOCYANIN.
DR SUPFAM; SSF49503; SSF49503; 1.
DR TIGRFAMs; TIGR02656; cyanin_plasto; 1.
DR PROSITE; PS00196; COPPER_BLUE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Copper; Direct protein sequencing; Electron transport;
KW Membrane; Metal-binding; Reference proteome; Signal; Thylakoid; Transport.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|PubMed:9298645"
FT CHAIN 29..126
FT /note="Plastocyanin"
FT /id="PRO_0000002904"
FT DOMAIN 29..126
FT /note="Plastocyanin-like"
FT BINDING 67
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:9466912"
FT BINDING 111
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:9466912"
FT BINDING 114
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:9466912"
FT BINDING 119
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:9466912"
FT STRAND 31..36
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 38..40
FT /evidence="ECO:0007829|PDB:1J5C"
FT STRAND 42..52
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 56..61
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:1J5D"
FT STRAND 67..71
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:1PCS"
FT HELIX 78..84
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 86..90
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 96..100
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 105..110
FT /evidence="ECO:0007829|PDB:1PCS"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:1PCS"
FT TURN 115..118
FT /evidence="ECO:0007829|PDB:1PCS"
FT STRAND 120..126
FT /evidence="ECO:0007829|PDB:1PCS"
SQ SEQUENCE 126 AA; 13146 MW; BA4E48109145C9EA CRC64;
MSKKFLTILA GLLLVVSSFF LSVSPAAAAN ATVKMGSDSG ALVFEPSTVT IKAGEEVKWV
NNKLSPHNIV FAADGVDADT AAKLSHKGLA FAAGESFTST FTEPGTYTYY CEPHRGAGMV
GKVVVE
