ID   PLBL2_RAT               Reviewed;         585 AA.
AC   Q4QQW8; A0JN10; Q3MHS4; Q5RJZ9; Q8K1I4;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 2.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Putative phospholipase B-like 2;
DE            EC=3.1.1.-;
DE   AltName: Full=LAMA-like protein 2;
DE   AltName: Full=Lamina ancestor homolog 2;
DE   AltName: Full=Phospholipase B domain-containing protein 2;
DE   Flags: Precursor;
GN   Name=Plbd2; ORFNames=RDCR-0918-3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-585.
RC   TISSUE=Brain, Liver, Ovary, and Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 320-585.
RC   STRAIN=Sprague-Dawley;
RA   Zhang F.L., Ye C.Z., Xie C., Li G., Luo M.;
RT   "Expression profile of cardiovascular complications in type 2 diabetes.";
RL   Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 351-363, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RA   Lubec G., Kang S.U., Lubec S.;
RL   Submitted (SEP-2007) to UniProtKB.
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=17105447; DOI=10.1042/bj20061205;
RA   Jensen A.G., Chemali M., Chapel A., Kieffer-Jaquinod S., Jadot M.,
RA   Garin J., Journet A.;
RT   "Biochemical characterization and lysosomal localization of the mannose-6-
RT   phosphate protein p76 (hypothetical protein LOC196463).";
RL   Biochem. J. 402:449-458(2007).
CC   -!- FUNCTION: Putative phospholipase. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with IGF2R. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Lysosome lumen {ECO:0000269|PubMed:17105447}.
CC   -!- PTM: Glycosylated; contains mannose 6-phosphate sugars. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the phospholipase B-like family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM23313.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AABR03082299; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC086408; AAH86408.1; -; mRNA.
DR   EMBL; BC097934; AAH97934.1; -; mRNA.
DR   EMBL; BC104715; AAI04716.1; -; mRNA.
DR   EMBL; BC126078; AAI26079.1; -; mRNA.
DR   EMBL; AY095481; AAM23313.1; ALT_INIT; mRNA.
DR   RefSeq; NP_640348.2; NM_139255.3.
DR   AlphaFoldDB; Q4QQW8; -.
DR   SMR; Q4QQW8; -.
DR   IntAct; Q4QQW8; 1.
DR   STRING; 10116.ENSRNOP00000001872; -.
DR   MEROPS; C95.001; -.
DR   GlyGen; Q4QQW8; 6 sites.
DR   jPOST; Q4QQW8; -.
DR   PaxDb; Q4QQW8; -.
DR   PRIDE; Q4QQW8; -.
DR   GeneID; 246120; -.
DR   KEGG; rno:246120; -.
DR   UCSC; RGD:708461; rat.
DR   CTD; 196463; -.
DR   RGD; 708461; Plbd2.
DR   VEuPathDB; HostDB:ENSRNOG00000001385; -.
DR   eggNOG; KOG3774; Eukaryota.
DR   HOGENOM; CLU_027106_4_0_1; -.
DR   InParanoid; Q4QQW8; -.
DR   OMA; FQWSKSP; -.
DR   OrthoDB; 724258at2759; -.
DR   PhylomeDB; Q4QQW8; -.
DR   TreeFam; TF315042; -.
DR   PRO; PR:Q4QQW8; -.
DR   Proteomes; UP000002494; Chromosome 12.
DR   Bgee; ENSRNOG00000001385; Expressed in liver and 19 other tissues.
DR   Genevisible; Q4QQW8; RN.
DR   GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR   GO; GO:0043202; C:lysosomal lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISO:RGD.
DR   GO; GO:0004620; F:phospholipase activity; IBA:GO_Central.
DR   GO; GO:0009395; P:phospholipid catabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.439.20; -; 1.
DR   Gene3D; 2.10.70.60; -; 1.
DR   Gene3D; 3.60.60.20; -; 1.
DR   InterPro; IPR007000; PLipase_B-like.
DR   InterPro; IPR043040; PLipase_B-like_dom1.
DR   InterPro; IPR043041; PLipase_B-like_dom2.
DR   InterPro; IPR043042; PLipase_B-like_dom3.
DR   PANTHER; PTHR12370; PTHR12370; 1.
DR   Pfam; PF04916; Phospholip_B; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW   Lipid degradation; Lipid metabolism; Lysosome; Reference proteome; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..585
FT                   /note="Putative phospholipase B-like 2"
FT                   /id="PRO_0000286112"
FT   CARBOHYD        84
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        102
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        106
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        227
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        432
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        511
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        138..148
FT                   /evidence="ECO:0000250"
FT   DISULFID        488..491
FT                   /evidence="ECO:0000250"
FT   CONFLICT        322
FT                   /note="I -> V (in Ref. 3; AAM23313)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        433
FT                   /note="A -> P (in Ref. 3; AAM23313)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        485
FT                   /note="L -> P (in Ref. 3; AAM23313)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        570
FT                   /note="G -> S (in Ref. 3; AAM23313)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   585 AA;  65456 MW;  66DC0BA818C606B1 CRC64;
     MAAPMDRTHG GRAARALRRA LALASLAGLL LSGLAGALPT LGPGWRRQNP EPPASRTRSL
     LLDAASGQLR LEYGFHPDAV AWANLTNAIR ETGWAYLDLG TNGSYNDSLQ AYAAGVVEAS
     VSEELIYMHW MNTVVNYCGP FEYEVGYCEK LKSFLEANLE WMQREMELSP DSPYWHQVRL
     TLLQLKGLED SYEGRLTFPT GRFNIKPLGF LLLQISGDLE DLEPALNKTN TKPSVGSGSC
     SALIKLLPGS HDLLVAHNTW NSYQNMLRII KKYRLQFREG PQEEYPLIAG NNLIFSSYPG
     TIFSGDDFYI LGSGLVTLET TIGNKNPALW KYVQPQGCVL EWIRNIVANR LALDGATWAD
     VFRRFNSGTY NNQWMIVDYK AFIPNGPSPG SRVLTILEQI PGMVVVADKT AELYKTTYWA
     SYNIPYFESV FNASGLQALV AQYGDWFSYT RNPRAKIFQR DQSLVEDVDT MVRLMRYNDF
     LHDPLSLCEA CSPKPNAENA ISARSDLNPA NGSYPFQALR QRAHGGIDVK VTSVALAKYM
     SMLAASGPTW DQLPPFQWSK SPFHNMLHMG QPDLWMFSPV KVPWD