PLB_NEUCR
ID PLB_NEUCR Reviewed; 653 AA.
AC O42790; O42791; Q6MUU7; Q7RW01;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Lysophospholipase;
DE EC=3.1.1.5;
DE AltName: Full=Phospholipase B;
DE Flags: Precursor;
GN Name=lpl; ORFNames=5C2.110, NCU03141;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ASN-45; VAL-115 AND
RP ASP-597.
RC STRAIN=74-OR23-1VA / FGSC 2489, and Lindegren 25a;
RA Catcheside D.E.A., Yeadon P.J.;
RT "Polymorphism around cog extends into adjacent structural genes.";
RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Catalyzes the release of fatty acids from lysophospholipids.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid +
CC H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870,
CC ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the lysophospholipase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC03053.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF045574; AAC03052.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AF045575; AAC03053.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BX842637; CAE76554.1; -; Genomic_DNA.
DR EMBL; CM002236; EAA34954.2; -; Genomic_DNA.
DR RefSeq; XP_964190.2; XM_959097.3.
DR AlphaFoldDB; O42790; -.
DR SMR; O42790; -.
DR STRING; 5141.EFNCRP00000002857; -.
DR EnsemblFungi; EAA34954; EAA34954; NCU03141.
DR GeneID; 3880339; -.
DR KEGG; ncr:NCU03141; -.
DR VEuPathDB; FungiDB:NCU03141; -.
DR HOGENOM; CLU_014602_0_0_1; -.
DR InParanoid; O42790; -.
DR OMA; FGTWDPT; -.
DR Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0004622; F:lysophospholipase activity; IEA:UniProtKB-EC.
DR GO; GO:0102545; F:phosphatidyl phospholipase B activity; IEA:UniProtKB-EC.
DR GO; GO:0004623; F:phospholipase A2 activity; IBA:GO_Central.
DR GO; GO:0046475; P:glycerophospholipid catabolic process; IBA:GO_Central.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR002642; LysoPLipase_cat_dom.
DR Pfam; PF01735; PLA2_B; 1.
DR SMART; SM00022; PLAc; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR PROSITE; PS51210; PLA2C; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..653
FT /note="Lysophospholipase"
FT /id="PRO_0000024638"
FT DOMAIN 57..606
FT /note="PLA2c"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00555"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 149
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 298
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 328
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 365
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 407
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 459
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 476
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 507
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 533
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 561
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 602
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 45
FT /note="D -> N (in strain: Lindegren 25a)"
FT /evidence="ECO:0000269|Ref.1"
FT VARIANT 115
FT /note="I -> V (in strain: Lindegren 25a)"
FT /evidence="ECO:0000269|Ref.1"
FT VARIANT 597
FT /note="E -> D (in strain: Lindegren 25a)"
FT /evidence="ECO:0000269|Ref.1"
SQ SEQUENCE 653 AA; 70111 MW; BF8EFCF1F6515A35 CRC64;
MHLPSSLLIA APLLANVSAE PIRIPQRDVS VVSTSQQLAV RALPDSPSGG YAPAVVDCPK
TKPTLRKAVD LSNEEKNWLS IRRKNTIQPM RDLLKRANIT GFDSETFMNE AANNISQLPN
VAIAISGGGY RALMNGAGFV AAADNRIQNT TGAGGIGGLL QSSTYLAGLS GGGWLVGSLF
SNNFSSIETL LSENKVWDFE NSIFKGPKEA GLSTVNRIQY WSEVAKEVAK KKDAGFETSI
TDYWGRALSY QLIGADMGGP AYTFSSIAQT DNFQKAETPF PILVADGRAP GDTIISLNAT
NYEFNPFETG SWDPTVYGFA PTKYLGANFS NGVIPSGGKC VEGLDQAGFV MGTSSTLFNQ
FLLANISSYD GVPDVLIEAV TSVLKEIGAK RDDVSQIIPN PFLDWNNRTN PNADTLELDL
VDGGEDLQNI PLNPLTQPVR AVDVIFAVDS SADVTNWPNG TALRATYERT FGSISNGTLF
PSIPDDWTFI NLGLNNRPSF FGCDVKNFTL NANQKVPPLI VYVPNAPYTA LSNVSTFDPS
YTMSQRNDII GNGWNSATQG NGTLDSEWPT CVACAVISRS LDRLGRQTPA ACKTCFERYC
WNGTVNSKDT GVYMPEFKIA DAHALDSGAV AIGKMVNVWS SVVVGVVAAT LLL
