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PLCA_BOVIN
ID   PLCA_BOVIN              Reviewed;         287 AA.
AC   Q95JH2; Q95MP8;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=1-acyl-sn-glycerol-3-phosphate acyltransferase alpha;
DE            EC=2.3.1.51 {ECO:0000250|UniProtKB:Q99943};
DE   AltName: Full=1-acylglycerol-3-phosphate O-acyltransferase 1;
DE            Short=1-AGP acyltransferase 1;
DE            Short=1-AGPAT 1;
DE   AltName: Full=Lysophosphatidic acid acyltransferase alpha {ECO:0000250|UniProtKB:Q99943};
DE            Short=LPAAT-alpha;
DE   Flags: Precursor;
GN   Name=AGPAT1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=Holstein; TISSUE=Mammary gland;
RX   PubMed=11860120; DOI=10.3168/jds.s0022-0302(02)74049-6;
RA   Mistry D.H., Medrano J.F.;
RT   "Cloning and localization of the bovine and ovine lysophosphatidic acid
RT   acyltransferase (LPAAT) genes that codes for an enzyme involved in
RT   triglyceride biosynthesis.";
RL   J. Dairy Sci. 85:28-35(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic
CC       acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid
CC       or PA) by incorporating an acyl moiety at the sn-2 position of the
CC       glycerol backbone. {ECO:0000250|UniProtKB:Q99943}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-
CC         sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:19709,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342,
CC         ChEBI:CHEBI:58608; EC=2.3.1.51;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19710;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC         phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37131, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:74546;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37132;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA
CC         = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37143, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:74551;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37144;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + heptadecanoyl-CoA
CC         = 1-(9Z)-octadecenoyl-2-heptadecanoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37155, ChEBI:CHEBI:57287, ChEBI:CHEBI:74307,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:74558;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37156;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + octadecanoyl-CoA
CC         = 1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37147, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:74552;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37148;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-
CC         3-phosphate = 1-(9Z)-octadecenoyl-2-(9Z,12Z)-octadecadienoyl-sn-
CC         glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37159, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57383, ChEBI:CHEBI:74544, ChEBI:CHEBI:74563;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37160;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + tetradecanoyl-CoA
CC         = 1-(9Z)-octadecenoyl-2-tetradecanoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37171, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:74579;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37172;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + pentadecanoyl-CoA
CC         = 1-(9Z)-octadecenoyl-2-pentadecanoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37175, ChEBI:CHEBI:57287, ChEBI:CHEBI:74309,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:74578;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37176;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate
CC         = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:33187, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:57518, ChEBI:CHEBI:64839;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33188;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z)-octadecatrienoyl-sn-
CC         glycero-3-phosphate = 1-(9Z,12Z,15Z)-octadecatrienoyl-2-(9Z)-
CC         octadecenoyl-sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37139,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:74549,
CC         ChEBI:CHEBI:74550; Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37140;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-(6Z,9Z,12Z-octadecatrienoyl)-sn-
CC         glycero-3-phosphate = (6Z,9Z,12Z)-octadecatrienoyl-2-(9Z)-
CC         octadecenoyl-sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37179,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:74581,
CC         ChEBI:CHEBI:74582; Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37180;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-eicosanoyl-sn-glycero-3-phosphate =
CC         1-eicosanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37183, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC         ChEBI:CHEBI:74583, ChEBI:CHEBI:74584;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37184;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + 1-tetradecanoyl-sn-glycerol 3-
CC         phosphate = 1-tetradecanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:37187, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57387, ChEBI:CHEBI:72683, ChEBI:CHEBI:74586;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37188;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-(9Z-octadecenoyl)-sn-
CC         glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(5Z,8Z,11Z,14Z)-
CC         eicosatetraenoyl-sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37443,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57368, ChEBI:CHEBI:74544,
CC         ChEBI:CHEBI:74928; Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37444;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + dodecanoyl-CoA =
CC         1-(9Z)-octadecenoyl-2-dodecanoyl-sn-glycero-3-phosphate + CoA;
CC         Xref=Rhea:RHEA:37591, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:75076;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37592;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC         phosphate = 1-(9Z)-octadecenoyl-2-(6Z)-octadecenoyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:37607, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:75123, ChEBI:CHEBI:75124;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37608;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(11Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC         phosphate = 1-(9Z)-octadecenoyl-2-(11Z)-octadecenoyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:37603, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:74544, ChEBI:CHEBI:75121, ChEBI:CHEBI:75122;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37604;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-hexadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC         phosphate = 1-(9Z-octadecenoyl)-2-(9Z-hexadecenoyl)-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:40195, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:61540, ChEBI:CHEBI:74544, ChEBI:CHEBI:74697;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40196;
CC         Evidence={ECO:0000250|UniProtKB:Q99943};
CC   -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC       diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q99943}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC       may constitute the binding site for the phosphate moiety of the
CC       glycerol-3-phosphate. {ECO:0000250|UniProtKB:Q9D517}.
CC   -!- SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate
CC       acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF281677; AAK69168.1; -; mRNA.
DR   EMBL; AF285100; AAK58832.1; -; Genomic_DNA.
DR   EMBL; BC115999; AAI16000.1; -; mRNA.
DR   RefSeq; NP_803484.1; NM_177518.1.
DR   RefSeq; XP_015315353.1; XM_015459867.1.
DR   RefSeq; XP_015315354.1; XM_015459868.1.
DR   AlphaFoldDB; Q95JH2; -.
DR   SMR; Q95JH2; -.
DR   STRING; 9913.ENSBTAP00000005821; -.
DR   PaxDb; Q95JH2; -.
DR   PeptideAtlas; Q95JH2; -.
DR   PRIDE; Q95JH2; -.
DR   Ensembl; ENSBTAT00000005821; ENSBTAP00000005821; ENSBTAG00000004442.
DR   Ensembl; ENSBTAT00000083979; ENSBTAP00000062109; ENSBTAG00000004442.
DR   GeneID; 282137; -.
DR   KEGG; bta:282137; -.
DR   CTD; 10554; -.
DR   VEuPathDB; HostDB:ENSBTAG00000004442; -.
DR   VGNC; VGNC:25734; AGPAT1.
DR   eggNOG; KOG2848; Eukaryota.
DR   GeneTree; ENSGT00390000008726; -.
DR   HOGENOM; CLU_027938_10_1_1; -.
DR   InParanoid; Q95JH2; -.
DR   OMA; KQRIKLW; -.
DR   OrthoDB; 1623097at2759; -.
DR   TreeFam; TF314867; -.
DR   Reactome; R-BTA-1483166; Synthesis of PA.
DR   Reactome; R-BTA-163765; ChREBP activates metabolic gene expression.
DR   UniPathway; UPA00557; UER00613.
DR   Proteomes; UP000009136; Chromosome 23.
DR   Bgee; ENSBTAG00000004442; Expressed in Ammon's horn and 106 other tissues.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003841; F:1-acylglycerol-3-phosphate O-acyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006654; P:phosphatidic acid biosynthetic process; IBA:GO_Central.
DR   GO; GO:0001819; P:positive regulation of cytokine production; IEA:Ensembl.
DR   InterPro; IPR004552; AGP_acyltrans.
DR   InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR   Pfam; PF01553; Acyltransferase; 1.
DR   SMART; SM00563; PlsC; 1.
DR   TIGRFAMs; TIGR00530; AGP_acyltrn; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Endoplasmic reticulum; Lipid biosynthesis;
KW   Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW   Phospholipid metabolism; Reference proteome; Signal; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..287
FT                   /note="1-acyl-sn-glycerol-3-phosphate acyltransferase
FT                   alpha"
FT                   /id="PRO_0000254018"
FT   TOPO_DOM        27..37
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:Q99943"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        59..127
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q99943"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        149..287
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:Q99943"
FT   MOTIF           104..109
FT                   /note="HXXXXD motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9D517"
FT   MOTIF           178..181
FT                   /note="EGTR motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q99943"
FT   CONFLICT        41
FT                   /note="Y -> C (in Ref. 1; AAK58832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        52
FT                   /note="A -> V (in Ref. 1; AAK58832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243
FT                   /note="K -> R (in Ref. 1; AAK58832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        271
FT                   /note="G -> S (in Ref. 1; AAK58832)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   287 AA;  32005 MW;  7D49534A61E07441 CRC64;
     MELWPGAGTL LLLLFLLLLL LLPTLWFCSP SAKYFFKMAF YNGWILFLAV LAIPVCAVRG
     RNVENMKILR LMLLHIKYLY GIRVEVRGAH HFPPSQPYVV VSNHQSSLDL LGMMEVLPGR
     CVPIAKRELL WAGSAGLACW LAGVIFIDRK RTGDAISVMS EVAQTLLTQD VRVWVFPEGT
     RNHNGSMLPF KRGAFHLAVQ AQVPIVPIVM SSYQDFYCKK ERRFTSGRCQ VRVLPPVPTE
     GLKPDDVPAL ADRVRHSMLT VFREISTDGR GGGDYLKKPG GVGEAGL
 
 
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