ID   PLC_STAA8               Reviewed;         328 AA.
AC   Q2G1Q2;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=1-phosphatidylinositol phosphodiesterase;
DE            EC=4.6.1.13;
DE   AltName: Full=Phosphatidylinositol diacylglycerol-lyase;
DE   AltName: Full=Phosphatidylinositol-specific phospholipase C;
DE            Short=PI-PLC;
DE   Flags: Precursor;
GN   Name=plc; OrderedLocusNames=SAOUHSC_00051;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=RN4220;
RX   PubMed=20472795; DOI=10.1128/jb.01452-09;
RA   Sibbald M.J., Winter T., van der Kooi-Pol M.M., Buist G., Tsompanidou E.,
RA   Bosma T., Schafer T., Ohlsen K., Hecker M., Antelmann H., Engelmann S.,
RA   van Dijl J.M.;
RT   "Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in
RT   Staphylococcus aureus.";
RL   J. Bacteriol. 192:3788-3800(2010).
CC   -!- FUNCTION: Cleaves glycosylphosphatidylinositol (GPI) and
CC       phosphatidylinositol (PI) anchors but not PI phosphates. Potential
CC       virulence factor (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) = 1D-myo-
CC         inositol 1,2-cyclic phosphate + a 1,2-diacyl-sn-glycerol;
CC         Xref=Rhea:RHEA:17093, ChEBI:CHEBI:17815, ChEBI:CHEBI:57880,
CC         ChEBI:CHEBI:58484; EC=4.6.1.13;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20472795}.
CC   -!- INDUCTION: Less protein is secreted in a secG or double secG/secY2
CC       mutant (at protein level). {ECO:0000269|PubMed:20472795}.
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DR   EMBL; CP000253; ABD29239.1; -; Genomic_DNA.
DR   RefSeq; WP_000710576.1; NZ_LS483365.1.
DR   RefSeq; YP_498656.1; NC_007795.1.
DR   AlphaFoldDB; Q2G1Q2; -.
DR   SMR; Q2G1Q2; -.
DR   STRING; 1280.SAXN108_0086; -.
DR   EnsemblBacteria; ABD29239; ABD29239; SAOUHSC_00051.
DR   GeneID; 3919083; -.
DR   KEGG; sao:SAOUHSC_00051; -.
DR   PATRIC; fig|93061.5.peg.45; -.
DR   eggNOG; COG0823; Bacteria.
DR   HOGENOM; CLU_024117_3_1_9; -.
DR   OMA; ITMPGTH; -.
DR   PRO; PR:Q2G1Q2; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004436; F:phosphatidylinositol diacylglycerol-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008081; F:phosphoric diester hydrolase activity; IBA:GO_Central.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.190; -; 1.
DR   InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR   InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR   Pfam; PF00388; PI-PLC-X; 1.
DR   SMART; SM00148; PLCXc; 1.
DR   SUPFAM; SSF51695; SSF51695; 1.
DR   PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Lyase; Reference proteome;
KW   Secreted; Signal; Transducer; Virulence.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..328
FT                   /note="1-phosphatidylinositol phosphodiesterase"
FT                   /id="PRO_0000414603"
FT   DOMAIN          41..192
FT                   /note="PI-PLC X-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00270"
FT   ACT_SITE        56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00270"
FT   ACT_SITE        106
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00270"
SQ   SEQUENCE   328 AA;  37087 MW;  3C02CB88A157028D CRC64;
     MKKCIKTLFL SIILVVMSGW YHSAHASDSL SKSPENWMSK LDDGKHLTEI NIPGSHDSGS
     FTLKDPVKSV WAKTQDKDYL TQMKSGVRFF DIRGRASADN MISVHHGMVY LHHELGKFLD
     DAKYYLSAYP NETIVMSMKK DYDSDSKVTK TFEEIFREYY YNNPQYQNLF YTGSNANPTL
     KETKGKIVLF NRMGGTYIKS GYGADTSGIQ WADNATFETK INNGSLNLKV QDEYKDYYDK
     KVEAVKNLLA KAKTDSNKDN VYVNFLSVAS GGSAFNSTYN YASHINPEIA KTIKANGKAR
     TGWLIVDYAG YTWPGYDDIV SEIIDSNK