ID   PLE2_PSEAM              Reviewed;          68 AA.
AC   Q90ZY0; Q90VW6;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Pleurocidin;
DE   Flags: Precursor;
GN   Name=ple2;
OS   Pseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Carangaria; Pleuronectiformes; Pleuronectoidei; Pleuronectidae;
OC   Pseudopleuronectes.
OX   NCBI_TaxID=8265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   TISSUE=Skin;
RX   PubMed=11113283; DOI=10.1016/s0145-305x(00)00052-5;
RA   Douglas S.E., Gallant J.W., Gong Z., Hew C.-L.;
RT   "Cloning and developmental expression of a family of pleurocidin-like
RT   antimicrobial peptides from winter flounder, Pleuronectes americanus
RT   (Walbaum).";
RL   Dev. Comp. Immunol. 25:137-147(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=12950255; DOI=10.1046/j.1432-1033.2003.03758.x;
RA   Douglas S.E., Patrzykat A., Pytyck J., Gallant J.W.;
RT   "Identification, structure and differential expression of novel
RT   pleurocidins clustered on the genome of the winter flounder,
RT   Pseudopleuronectes americanus (Walbaum).";
RL   Eur. J. Biochem. 270:3720-3730(2003).
RN   [3]
RP   PROTEIN SEQUENCE OF 23-47, AND CHARACTERIZATION.
RC   TISSUE=Epidermis, and Skin mucus;
RX   PubMed=9115266; DOI=10.1074/jbc.272.18.12008;
RA   Cole A.M., Weis P., Diamond G.;
RT   "Isolation and characterization of pleurocidin, an antimicrobial peptide in
RT   the skin secretions of winter flounder.";
RL   J. Biol. Chem. 272:12008-12013(1997).
CC   -!- FUNCTION: Antimicrobial peptide with potent activity against Gram-
CC       positive and Gram-negative bacteria. Activity against E.coli and
CC       B.subtilis. Weaker activity against L.mucor, s.marcescens and
CC       P.aeruginosa. May play a role in innate host defense.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Goblet cells.
CC   -!- SIMILARITY: Belongs to the pleurocidin family. {ECO:0000305}.
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DR   EMBL; AF301507; AAK52842.1; -; mRNA.
DR   EMBL; AF301511; AAK52846.1; -; Genomic_DNA.
DR   EMBL; AF301512; AAK52847.1; -; Genomic_DNA.
DR   EMBL; AY282499; AAQ16625.1; -; Genomic_DNA.
DR   PDB; 6RSF; NMR; -; A=23-47.
DR   PDBsum; 6RSF; -.
DR   AlphaFoldDB; Q90ZY0; -.
DR   BMRB; Q90ZY0; -.
DR   SMR; Q90ZY0; -.
DR   TCDB; 1.C.62.1.1; the pseudopleuronectes americanus (flounder) pleurocidin (pleurocidin) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR012515; Antimicrobial12.
DR   Pfam; PF08107; Antimicrobial12; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:9115266"
FT   PEPTIDE         23..47
FT                   /note="Pleurocidin"
FT                   /id="PRO_0000000275"
FT   PROPEP          48..68
FT                   /id="PRO_0000000276"
FT   CONFLICT        44..45
FT                   /note="TH -> HT (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   HELIX           29..31
FT                   /evidence="ECO:0007829|PDB:6RSF"
FT   TURN            32..35
FT                   /evidence="ECO:0007829|PDB:6RSF"
FT   TURN            37..40
FT                   /evidence="ECO:0007829|PDB:6RSF"
SQ   SEQUENCE   68 AA;  7583 MW;  0601CFAE55C6E19D CRC64;
     MKFTATFLMI AIFVLMVEPG ECGWGSFFKK AAHVGKHVGK AALTHYLGDK QELNKRAVDE
     DPNVIVFE