ID   PLEA2_NIDPL             Reviewed;          69 AA.
AC   A8B5P0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Pleurain-A2;
DE   Flags: Precursor;
OS   Nidirana pleuraden (Yunnan pond frog) (Babina pleuraden).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Nidirana.
OX   NCBI_TaxID=369511;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 44-69, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Skin, and Skin secretion;
RX   PubMed=17764786; DOI=10.1016/j.peptides.2007.07.020;
RA   Wang X., Song Y., Li J., Liu H., Xu X., Lai R., Zhang K.;
RT   "A new family of antimicrobial peptides from skin secretions of Rana
RT   pleuraden.";
RL   Peptides 28:2069-2074(2007).
CC   -!- FUNCTION: Antimicrobial peptide. Has activity against the Gram-positive
CC       bacterium S.aureus ATCC2592 (MIC=15 ug/ml), the Gram-negative bacteria
CC       E.coli ATCC25922 (MIC=60 ug/ml), B.dysenteriae (MIC=60 ug/ml), H.pylori
CC       NTCT11637 (MIC=30 ug/ml), and the fungus C.albicans ATCC2002 (MIC=30
CC       ug/ml). Has little hemolytic activity on rabbit red blood cells.
CC       {ECO:0000269|PubMed:17764786}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17764786}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:17764786}.
CC   -!- MASS SPECTROMETRY: Mass=3024.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17764786};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Pleurain subfamily. {ECO:0000305}.
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DR   EMBL; EF621707; ABU95403.1; -; mRNA.
DR   AlphaFoldDB; A8B5P0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Cytolysis; Direct protein sequencing;
KW   Disulfide bond; Hemolysis; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..43
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000314000"
FT   PEPTIDE         44..69
FT                   /note="Pleurain-A2"
FT                   /id="PRO_0000314001"
FT   DISULFID        63..69
SQ   SEQUENCE   69 AA;  8075 MW;  E6062439CD84482F CRC64;
     MFTLKKTLLL LYFLGTISIS LCKQERDADE DDGRKMTEEE VKRSIITMTK EAKLPQSWKQ
     IACRLYNTC