ID   PLEA4_NIDPL             Reviewed;          69 AA.
AC   A8B5Q0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Pleurain-A4;
DE   Flags: Precursor;
OS   Nidirana pleuraden (Yunnan pond frog) (Babina pleuraden).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Nidirana.
OX   NCBI_TaxID=369511;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Skin;
RX   PubMed=17764786; DOI=10.1016/j.peptides.2007.07.020;
RA   Wang X., Song Y., Li J., Liu H., Xu X., Lai R., Zhang K.;
RT   "A new family of antimicrobial peptides from skin secretions of Rana
RT   pleuraden.";
RL   Peptides 28:2069-2074(2007).
CC   -!- FUNCTION: Antimicrobial peptide. Has activity against Gram-positive and
CC       -negative bacteria, and fungi. Has little hemolytic activity on red
CC       blood cells (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Pleurain subfamily. {ECO:0000305}.
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DR   EMBL; EF621709; ABU95405.1; -; mRNA.
DR   AlphaFoldDB; A8B5Q0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   2: Evidence at transcript level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Cytolysis; Disulfide bond; Hemolysis;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..43
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000314004"
FT   PEPTIDE         44..69
FT                   /note="Pleurain-A4"
FT                   /id="PRO_0000314005"
FT   DISULFID        63..69
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   69 AA;  8055 MW;  E6193B39CA2AFFDF CRC64;
     MFTLKKTLLL LFFLGTISIS LCKQERDADE DDGRKMTEEE VKRSIITTTK EAKLPQLWKQ
     IACRLYNTC