PLEK2_HUMAN
ID PLEK2_HUMAN Reviewed; 353 AA.
AC Q9NYT0; Q96JT0;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Pleckstrin-2;
GN Name=PLEK2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Inazu T.;
RT "Homo sapiens pleckstrin 2: cloning and characterization.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cervix, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [5]
RP STRUCTURE BY NMR OF 238-353.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the C-terminal PH domain of human pleckstrin 2.";
RL Submitted (OCT-2005) to the PDB data bank.
CC -!- FUNCTION: May help orchestrate cytoskeletal arrangement. Contribute to
CC lamellipodia formation.
CC -!- SUBCELLULAR LOCATION: Cell projection, lamellipodium membrane;
CC Peripheral membrane protein. Cytoplasm, cytoskeleton.
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DR EMBL; AF228603; AAF34791.1; -; mRNA.
DR EMBL; BC001226; AAH01226.1; -; mRNA.
DR EMBL; BC008056; AAH08056.2; -; mRNA.
DR CCDS; CCDS9782.1; -.
DR RefSeq; NP_057529.1; NM_016445.2.
DR PDB; 1X1G; NMR; -; A=238-353.
DR PDBsum; 1X1G; -.
DR AlphaFoldDB; Q9NYT0; -.
DR SMR; Q9NYT0; -.
DR BioGRID; 117708; 10.
DR STRING; 9606.ENSP00000216446; -.
DR iPTMnet; Q9NYT0; -.
DR PhosphoSitePlus; Q9NYT0; -.
DR BioMuta; PLEK2; -.
DR DMDM; 20532216; -.
DR EPD; Q9NYT0; -.
DR jPOST; Q9NYT0; -.
DR MassIVE; Q9NYT0; -.
DR MaxQB; Q9NYT0; -.
DR PaxDb; Q9NYT0; -.
DR PeptideAtlas; Q9NYT0; -.
DR PRIDE; Q9NYT0; -.
DR ProteomicsDB; 83274; -.
DR Antibodypedia; 96; 86 antibodies from 19 providers.
DR DNASU; 26499; -.
DR Ensembl; ENST00000216446.9; ENSP00000216446.4; ENSG00000100558.9.
DR GeneID; 26499; -.
DR KEGG; hsa:26499; -.
DR MANE-Select; ENST00000216446.9; ENSP00000216446.4; NM_016445.3; NP_057529.1.
DR UCSC; uc001xjh.2; human.
DR CTD; 26499; -.
DR DisGeNET; 26499; -.
DR GeneCards; PLEK2; -.
DR HGNC; HGNC:19238; PLEK2.
DR HPA; ENSG00000100558; Tissue enhanced (adrenal).
DR MIM; 608007; gene.
DR neXtProt; NX_Q9NYT0; -.
DR OpenTargets; ENSG00000100558; -.
DR PharmGKB; PA134963543; -.
DR VEuPathDB; HostDB:ENSG00000100558; -.
DR eggNOG; ENOG502QYJ8; Eukaryota.
DR GeneTree; ENSGT00940000157229; -.
DR HOGENOM; CLU_067828_0_0_1; -.
DR InParanoid; Q9NYT0; -.
DR OMA; ITKNDIH; -.
DR OrthoDB; 717547at2759; -.
DR PhylomeDB; Q9NYT0; -.
DR TreeFam; TF332246; -.
DR PathwayCommons; Q9NYT0; -.
DR BioGRID-ORCS; 26499; 15 hits in 1076 CRISPR screens.
DR ChiTaRS; PLEK2; human.
DR EvolutionaryTrace; Q9NYT0; -.
DR GeneWiki; PLEK2; -.
DR GenomeRNAi; 26499; -.
DR Pharos; Q9NYT0; Tbio.
DR PRO; PR:Q9NYT0; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q9NYT0; protein.
DR Bgee; ENSG00000100558; Expressed in oocyte and 148 other tissues.
DR ExpressionAtlas; Q9NYT0; baseline and differential.
DR Genevisible; Q9NYT0; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IEA:Ensembl.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IEA:Ensembl.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:Ensembl.
DR GO; GO:0031532; P:actin cytoskeleton reorganization; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0120034; P:positive regulation of plasma membrane bounded cell projection assembly; IEA:Ensembl.
DR CDD; cd04444; DEP_PLEK2; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR037369; PLEK2_DEP.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00169; PH; 2.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00233; PH; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell membrane; Cell projection; Cytoplasm;
KW Cytoskeleton; Membrane; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..353
FT /note="Pleckstrin-2"
FT /id="PRO_0000053862"
FT DOMAIN 4..104
FT /note="PH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 139..225
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 247..353
FT /note="PH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 120
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 80
FT /note="T -> M (in dbSNP:rs34300264)"
FT /id="VAR_050504"
FT STRAND 246..257
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 259..275
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 277..281
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 283..285
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 294..296
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 306..309
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 322..324
FT /evidence="ECO:0007829|PDB:1X1G"
FT STRAND 332..334
FT /evidence="ECO:0007829|PDB:1X1G"
FT HELIX 339..352
FT /evidence="ECO:0007829|PDB:1X1G"
SQ SEQUENCE 353 AA; 39971 MW; CE355BF917F2385E CRC64;
MEDGVLKEGF LVKRGHIVHN WKARWFILRQ NTLVYYKLEG GRRVTPPKGR ILLDGCTITC
PCLEYENRPL LIKLKTQTST EYFLEACSRE ERDAWAFEIT GAIHAGQPGK VQQLHSLRNS
FKLPPHISLH RIVDKMHDSN TGIRSSPNME QGSTYKKTFL GSSLVDWLIS NSFTASRLEA
VTLASMLMEE NFLRPVGVRS MGAIRSGDLA EQFLDDSTAL YTFAESYKKK ISPKEEISLS
TVELSGTVVK QGYLAKQGHK RKNWKVRRFV LRKDPAFLHY YDPSKEENRP VGGFSLRGSL
VSALEDNGVP TGVKGNVQGN LFKVITKDDT HYYIQASSKA ERAEWIEAIK KLT
