PLEK2_MOUSE
ID PLEK2_MOUSE Reviewed; 353 AA.
AC Q9WV52;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Pleckstrin-2;
GN Name=Plek2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10419454; DOI=10.1074/jbc.274.31.21515;
RA Hu M., Bauman E.M., Roll R.L., Yeilding N., Abrams C.S.;
RT "Pleckstrin 2, a widely expressed paralog of pleckstrin involved in actin
RT rearrangement.";
RL J. Biol. Chem. 274:21515-21518(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10548495; DOI=10.1006/bbrc.1999.1461;
RA Inazu T., Yamada K., Miyamoto K.;
RT "Cloning and expression of pleckstrin 2, a novel member of the pleckstrin
RT family.";
RL Biochem. Biophys. Res. Commun. 265:87-93(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP STRUCTURE BY NMR OF 129-235.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the DEP domain of mouse pleckstrin2.";
RL Submitted (APR-2004) to the PDB data bank.
CC -!- FUNCTION: May help orchestrate cytoskeletal arrangement. Contribute to
CC lamellipodia formation. Overexpression of pleckstrin 2 causes large
CC lamellipodia and peripheral ruffle formation.
CC -!- SUBCELLULAR LOCATION: Cell projection, lamellipodium membrane;
CC Peripheral membrane protein. Cytoplasm, cytoskeleton.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Most abundant in the thymus, large
CC bowel, small bowel, stomach, and prostate.
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DR EMBL; AF157600; AAD42973.1; -; mRNA.
DR EMBL; AF170564; AAD46924.1; -; mRNA.
DR EMBL; BC028902; AAH28902.1; -; mRNA.
DR CCDS; CCDS26004.1; -.
DR PIR; JC7128; JC7128.
DR RefSeq; NP_038766.1; NM_013738.3.
DR PDB; 1V3F; NMR; -; A=129-235.
DR PDBsum; 1V3F; -.
DR AlphaFoldDB; Q9WV52; -.
DR SMR; Q9WV52; -.
DR STRING; 10090.ENSMUSP00000021544; -.
DR iPTMnet; Q9WV52; -.
DR PhosphoSitePlus; Q9WV52; -.
DR MaxQB; Q9WV52; -.
DR PaxDb; Q9WV52; -.
DR PeptideAtlas; Q9WV52; -.
DR PRIDE; Q9WV52; -.
DR ProteomicsDB; 288250; -.
DR Antibodypedia; 96; 86 antibodies from 19 providers.
DR DNASU; 27260; -.
DR Ensembl; ENSMUST00000021544; ENSMUSP00000021544; ENSMUSG00000021118.
DR GeneID; 27260; -.
DR KEGG; mmu:27260; -.
DR UCSC; uc007nzl.2; mouse.
DR CTD; 26499; -.
DR MGI; MGI:1351466; Plek2.
DR VEuPathDB; HostDB:ENSMUSG00000021118; -.
DR eggNOG; ENOG502QYJ8; Eukaryota.
DR GeneTree; ENSGT00940000157229; -.
DR HOGENOM; CLU_067828_0_0_1; -.
DR InParanoid; Q9WV52; -.
DR OMA; ITKNDIH; -.
DR OrthoDB; 717547at2759; -.
DR PhylomeDB; Q9WV52; -.
DR TreeFam; TF332246; -.
DR BioGRID-ORCS; 27260; 3 hits in 76 CRISPR screens.
DR ChiTaRS; Plek2; mouse.
DR EvolutionaryTrace; Q9WV52; -.
DR PRO; PR:Q9WV52; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q9WV52; protein.
DR Bgee; ENSMUSG00000021118; Expressed in dorsal pancreas and 121 other tissues.
DR Genevisible; Q9WV52; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IDA:MGI.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IDA:MGI.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:MGI.
DR GO; GO:0030036; P:actin cytoskeleton organization; IMP:MGI.
DR GO; GO:0031532; P:actin cytoskeleton reorganization; IDA:MGI.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0031346; P:positive regulation of cell projection organization; IMP:MGI.
DR GO; GO:0120034; P:positive regulation of plasma membrane bounded cell projection assembly; IDA:MGI.
DR CDD; cd04444; DEP_PLEK2; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR037369; PLEK2_DEP.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00169; PH; 2.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00233; PH; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell membrane; Cell projection; Cytoplasm;
KW Cytoskeleton; Membrane; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..353
FT /note="Pleckstrin-2"
FT /id="PRO_0000053863"
FT DOMAIN 4..104
FT /note="PH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 139..225
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 247..353
FT /note="PH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYT0"
FT MOD_RES 120
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYT0"
FT HELIX 129..136
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 139..142
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 150..153
FT /evidence="ECO:0007829|PDB:1V3F"
FT HELIX 161..170
FT /evidence="ECO:0007829|PDB:1V3F"
FT HELIX 177..190
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 192..195
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 197..200
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 216..218
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 220..223
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 225..227
FT /evidence="ECO:0007829|PDB:1V3F"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:1V3F"
SQ SEQUENCE 353 AA; 40018 MW; 4593DC41B62E9A5A CRC64;
MEDGVLKEGF LVKRGHIVHN WKARWFILRQ NTLLYYKLEG GRRVTPPKGR IVLDGCTITC
PCLEYENRPL LIKLKTRTST EYFLEACSRE ERDSWAFEIT GAIHAGQPGK IQQLHILKNS
FKLPPHISLH RIVDKMHDTS TGIRPSPNME QGSTYKKTFL GSSLVDWLIS SNFAASRLEA
VTLASMLMEE NFLRPVGVRS MGAIRSGDLA EQFLDDSTAL YTFAESYKKK VSSKEEISLS
TMELSGTVVK QGYLSKQGHK RKNWKVRRFV LRKDPAFLHY YDPSKEENRP VGGFSLRGSL
VSALEDNGVP TGVKGNVQGN LFKVITKDDT HYYIQASSKA ERAEWIEAIK KLT
