PLEK_RAT
ID PLEK_RAT Reviewed; 350 AA.
AC Q4KM33;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Pleckstrin;
GN Name=Plek;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Major protein kinase C substrate of platelets. {ECO:0000250}.
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DR EMBL; BC098846; AAH98846.1; -; mRNA.
DR RefSeq; NP_001020921.1; NM_001025750.1.
DR RefSeq; XP_006251582.1; XM_006251520.1.
DR AlphaFoldDB; Q4KM33; -.
DR SMR; Q4KM33; -.
DR STRING; 10116.ENSRNOP00000007044; -.
DR iPTMnet; Q4KM33; -.
DR PhosphoSitePlus; Q4KM33; -.
DR PaxDb; Q4KM33; -.
DR Ensembl; ENSRNOT00000007044; ENSRNOP00000007044; ENSRNOG00000005214.
DR Ensembl; ENSRNOT00000094345; ENSRNOP00000083092; ENSRNOG00000005214.
DR GeneID; 364206; -.
DR KEGG; rno:364206; -.
DR UCSC; RGD:1308269; rat.
DR CTD; 5341; -.
DR RGD; 1308269; Plek.
DR eggNOG; ENOG502QQIA; Eukaryota.
DR GeneTree; ENSGT00940000157885; -.
DR HOGENOM; CLU_067828_0_0_1; -.
DR InParanoid; Q4KM33; -.
DR OMA; MEPKQIR; -.
DR OrthoDB; 717547at2759; -.
DR PhylomeDB; Q4KM33; -.
DR TreeFam; TF332246; -.
DR Reactome; R-RNO-114608; Platelet degranulation.
DR PRO; PR:Q4KM33; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000005214; Expressed in spleen and 19 other tissues.
DR ExpressionAtlas; Q4KM33; baseline and differential.
DR Genevisible; Q4KM33; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0032587; C:ruffle membrane; ISO:RGD.
DR GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0005080; F:protein kinase C binding; ISO:RGD.
DR GO; GO:0031532; P:actin cytoskeleton reorganization; ISO:RGD.
DR GO; GO:0030030; P:cell projection organization; ISO:RGD.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; ISO:RGD.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; ISO:RGD.
DR GO; GO:0007229; P:integrin-mediated signaling pathway; ISO:RGD.
DR GO; GO:0045744; P:negative regulation of G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0010920; P:negative regulation of inositol phosphate biosynthetic process; ISO:RGD.
DR GO; GO:0046488; P:phosphatidylinositol metabolic process; ISO:RGD.
DR GO; GO:0030845; P:phospholipase C-inhibiting G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0070527; P:platelet aggregation; ISO:RGD.
DR GO; GO:0002576; P:platelet degranulation; ISO:RGD.
DR GO; GO:0032233; P:positive regulation of actin filament bundle assembly; ISO:RGD.
DR GO; GO:0030836; P:positive regulation of actin filament depolymerization; ISO:RGD.
DR GO; GO:0010925; P:positive regulation of inositol-polyphosphate 5-phosphatase activity; ISO:RGD.
DR GO; GO:0033625; P:positive regulation of integrin activation; ISO:RGD.
DR GO; GO:0010572; P:positive regulation of platelet activation; ISO:RGD.
DR GO; GO:0070528; P:protein kinase C signaling; ISO:RGD.
DR GO; GO:0070560; P:protein secretion by platelet; ISO:RGD.
DR GO; GO:0060305; P:regulation of cell diameter; ISO:RGD.
DR GO; GO:0031529; P:ruffle organization; ISO:RGD.
DR GO; GO:0070493; P:thrombin-activated receptor signaling pathway; ISO:RGD.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; ISO:RGD.
DR CDD; cd04445; DEP_PLEK1; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR037371; PLEK_DEP.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00169; PH; 2.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00233; PH; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
PE 1: Evidence at protein level;
KW Acetylation; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..350
FT /note="Pleckstrin"
FT /id="PRO_0000053861"
FT DOMAIN 4..101
FT /note="PH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 136..221
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 244..347
FT /note="PH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT MOD_RES 64
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P08567"
FT MOD_RES 113
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P08567"
FT MOD_RES 117
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 350 AA; 39996 MW; F875E06531A24397 CRC64;
MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNNPKGMIPL KGSTLTSPCQ
DFGKRMFVLK ITTTKQQDHF FQAAYLEERD AWVRDIKKAI KCIEGGQKFA RKSTRRSIRL
PETIDLGALY LSMKDPEKGI KELNLEKDKK VFNHCFTGSG VIDWLVSNKL VRNRQEGLMI
SASLLSEGYL QPASDLSKNA ADGIAENPFL DNPDAFYYFP DSGFFCEENS SDDDIILKEE
FRGIIIKQGC LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGGEDPLGAI HLRGCVVTSV
ESNPDGKKSD EENLFEIITA DEVHYYMQAA TAKERTEWIK AIQVASRTGK
