PLF4_MOUSE
ID PLF4_MOUSE Reviewed; 105 AA.
AC Q9Z126;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Platelet factor 4;
DE Short=PF-4;
DE AltName: Full=C-X-C motif chemokine 4;
DE Flags: Precursor;
GN Name=Pf4; Synonyms=Cxcl4, Scyb4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=10319581; DOI=10.1007/s100380050136;
RA Watanabe O., Natori K., Tamari M., Shiomoto Y., Kubo S., Nakamura Y.;
RT "Significantly elevated expression of PF4 (platelet factor 4) and eotaxin
RT in the NOA mouse, a model for atopic dermatitis.";
RL J. Hum. Genet. 44:173-176(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11468158; DOI=10.1182/blood.v98.3.610;
RA Zhang C., Thornton M.A., Kowalska M.A., Sachis B.S., Feldman M., Poncz M.,
RA McKenzie S.E., Reilly M.P.;
RT "Localization of distal regulatory domains in the megakaryocyte-specific
RT platelet basic protein/platelet factor 4 gene locus.";
RL Blood 98:610-617(2001).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Released during platelet aggregation. Neutralizes the
CC anticoagulant effect of heparin because it binds more strongly to
CC heparin than to the chondroitin-4-sulfate chains of the carrier
CC molecule. Chemotactic for neutrophils and monocytes. Inhibits
CC endothelial cell proliferation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homotetramer. Interacts with TNFAIP6 (via Link domain).
CC {ECO:0000250|UniProtKB:P02776}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; AB017491; BAA75660.1; -; mRNA.
DR EMBL; AF349465; AAK30163.1; -; Genomic_DNA.
DR CCDS; CCDS19416.1; -.
DR RefSeq; NP_064316.1; NM_019932.4.
DR AlphaFoldDB; Q9Z126; -.
DR SMR; Q9Z126; -.
DR DIP; DIP-61569N; -.
DR IntAct; Q9Z126; 3.
DR STRING; 10090.ENSMUSP00000031320; -.
DR GlyGen; Q9Z126; 1 site.
DR iPTMnet; Q9Z126; -.
DR PhosphoSitePlus; Q9Z126; -.
DR CPTAC; non-CPTAC-5615; -.
DR jPOST; Q9Z126; -.
DR MaxQB; Q9Z126; -.
DR PaxDb; Q9Z126; -.
DR PeptideAtlas; Q9Z126; -.
DR PRIDE; Q9Z126; -.
DR ProteomicsDB; 289763; -.
DR DNASU; 56744; -.
DR Ensembl; ENSMUST00000031320; ENSMUSP00000031320; ENSMUSG00000029373.
DR GeneID; 56744; -.
DR KEGG; mmu:56744; -.
DR UCSC; uc008ybi.2; mouse.
DR CTD; 5196; -.
DR MGI; MGI:1888711; Pf4.
DR VEuPathDB; HostDB:ENSMUSG00000029373; -.
DR eggNOG; ENOG502TF57; Eukaryota.
DR GeneTree; ENSGT00940000163368; -.
DR HOGENOM; CLU_143902_1_2_1; -.
DR InParanoid; Q9Z126; -.
DR OMA; CAVPQLI; -.
DR OrthoDB; 1617719at2759; -.
DR PhylomeDB; Q9Z126; -.
DR TreeFam; TF333433; -.
DR Reactome; R-MMU-114608; Platelet degranulation.
DR Reactome; R-MMU-140875; Common Pathway of Fibrin Clot Formation.
DR Reactome; R-MMU-202733; Cell surface interactions at the vascular wall.
DR Reactome; R-MMU-380108; Chemokine receptors bind chemokines.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 56744; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q9Z126; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9Z126; protein.
DR Bgee; ENSMUSG00000029373; Expressed in femorotibial joint and 197 other tissues.
DR ExpressionAtlas; Q9Z126; baseline and differential.
DR Genevisible; Q9Z126; MM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0031091; C:platelet alpha granule; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0031982; C:vesicle; ISO:MGI.
DR GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0048248; F:CXCR3 chemokine receptor binding; ISS:UniProtKB.
DR GO; GO:0008201; F:heparin binding; ISS:UniProtKB.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IMP:UniProtKB.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0042832; P:defense response to protozoan; IMP:UniProtKB.
DR GO; GO:0006955; P:immune response; ISO:MGI.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0030595; P:leukocyte chemotaxis; ISS:UniProtKB.
DR GO; GO:0016525; P:negative regulation of angiogenesis; ISS:UniProtKB.
DR GO; GO:0045653; P:negative regulation of megakaryocyte differentiation; ISS:UniProtKB.
DR GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR GO; GO:0030168; P:platelet activation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; ISO:MGI.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISS:UniProtKB.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR InterPro; IPR027222; PF4.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR PANTHER; PTHR10179:SF43; PTHR10179:SF43; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 1: Evidence at protein level;
KW Chemotaxis; Cytokine; Disulfide bond; Glycoprotein; Heparin-binding;
KW Phosphoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000250"
FT CHAIN 30..105
FT /note="Platelet factor 4"
FT /id="PRO_0000005069"
FT BINDING 96..102
FT /ligand="heparin"
FT /ligand_id="ChEBI:CHEBI:28304"
FT /evidence="ECO:0000250"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P06765"
FT CARBOHYD 31
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250"
FT DISULFID 44..71
FT /evidence="ECO:0000250"
FT DISULFID 46..87
FT /evidence="ECO:0000250"
SQ SEQUENCE 105 AA; 11243 MW; 94F0190EFACF1A6C CRC64;
MSVAAVFRGL RPSPELLLLG LLFLPAVVAV TSAGPEESDG DLSCVCVKTI SSGIHLKHIT
SLEVIKAGRH CAVPQLIATL KNGRKICLDR QAPLYKKVIK KILES
