PLGRF_HUMAN
ID PLGRF_HUMAN Reviewed; 260 AA.
AC A0A3B3IS91;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 07-APR-2021, sequence version 2.
DT 03-AUG-2022, entry version 15.
DE RecName: Full=POLG alternative reading frame {ECO:0000303|PubMed:32958672};
DE Short=POLGARF {ECO:0000303|PubMed:32958672};
DE AltName: Full=ORF-Y {ECO:0000303|PubMed:32138667};
DE Contains:
DE RecName: Full=POLGARF C-terminal fragment {ECO:0000303|PubMed:32958672};
DE Short=POLGARFin {ECO:0000303|PubMed:32958672};
GN Name=POLG {ECO:0000312|HGNC:HGNC:9179};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606 {ECO:0000312|Proteomes:UP000005640};
RN [1] {ECO:0000312|Proteomes:UP000005640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 57-100; 142-150 AND 224-249, INTERACTION WITH C1QBP,
RP SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=32958672; DOI=10.1073/pnas.2001433117;
RA Loughran G., Zhdanov A.V., Mikhaylova M.S., Rozov F.N., Datskevich P.N.,
RA Kovalchuk S.I., Serebryakova M.V., Kiniry S.J., Michel A.M.,
RA O'Connor P.B.F., Papkovsky D.B., Atkins J.F., Baranov P.V., Shatsky I.N.,
RA Andreev D.E.;
RT "Unusually efficient CUG initiation of an overlapping reading frame in POLG
RT mRNA yields novel protein POLGARF.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:24936-24946(2020).
RN [3] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=32138667; DOI=10.1186/s12863-020-0828-7;
RA Khan Y.A., Jungreis I., Wright J.C., Mudge J.M., Choudhary J.S.,
RA Firth A.E., Kellis M.;
RT "Evidence for a novel overlapping coding sequence in POLG initiated at a
RT CUG start codon.";
RL BMC Genet. 21:25-25(2020).
CC -!- SUBUNIT: Interacts with C1QBP; the interaction results in nucleolar
CC localization of C1QBP, probably due to prevention of C1QBP maturation
CC and redirection from mitochondria to nucleoli.
CC {ECO:0000269|PubMed:32958672}.
CC -!- SUBCELLULAR LOCATION: [POLG alternative reading frame]: Nucleus,
CC nucleolus {ECO:0000269|PubMed:32958672}.
CC -!- SUBCELLULAR LOCATION: [POLGARF C-terminal fragment]: Secreted
CC {ECO:0000269|PubMed:32958672}.
CC -!- PTM: Undergoes proteolytic cleavage to produce a secreted C-terminal
CC fragment. {ECO:0000269|PubMed:32958672}.
CC -!- CAUTION: Encoded by an alternative reading frame in the POLG gene.
CC Translation initiation occurs at a non-canonical CUG codon.
CC {ECO:0000269|PubMed:32138667, ECO:0000269|PubMed:32958672}.
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DR EMBL; AC124068; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR Antibodypedia; 28558; 218 antibodies from 33 providers.
DR Ensembl; ENST00000650303.2; ENSP00000497242.2; ENSG00000140521.17.
DR GeneReviews; POLG; -.
DR HGNC; HGNC:9179; POLG.
DR OpenTargets; ENSG00000140521; -.
DR VEuPathDB; HostDB:ENSG00000140521; -.
DR GeneTree; ENSGT00390000000453; -.
DR ChiTaRS; POLG; human.
DR Proteomes; UP000005640; Chromosome 15.
DR Bgee; ENSG00000140521; Expressed in granulocyte and 210 other tissues.
DR ExpressionAtlas; A0A3B3IS91; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Nucleus; Reference proteome; Secreted.
FT CHAIN 1..260
FT /note="POLG alternative reading frame"
FT /id="PRO_0000454209"
FT CHAIN ?..260
FT /note="POLGARF C-terminal fragment"
FT /evidence="ECO:0000269|PubMed:32958672"
FT /id="PRO_0000454210"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..130
FT /note="Required for nucleolar localization"
FT /evidence="ECO:0000269|PubMed:32958672"
FT REGION 108..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..166
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 260 AA; 25527 MW; 8A9AAD99BB34107A CRC64;
MEPKARCSDS QRGGPCTNHE PPALEEGGRR HRRARAGSSS GALGLQLRPR VRPQRRAAAA
AAAAAAAAAA ATAASAAASA ILGGRAAAAQ PIGHPDALER AARANLRARR GDAWRGRGAP
QRRAPAEARA LGAASRALAR RGAAPAAPLR GQPGPALPPP GPEAEPALPG GGQLAVAGPA
APEAPGLGLG GGLDPVRPRG GGRTRGHPRG AGPGVRRGGL LGRGNLPHIG GGHIPLGLVF
LVQPAAGGRA LLLDQPAVAG
