ASTER_MOUSE
ID ASTER_MOUSE Reviewed; 106 AA.
AC Q6ZWX0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=PAT complex subunit Asterix {ECO:0000250|UniProtKB:Q9Y284};
DE AltName: Full=Protein WDR83OS homolog;
DE AltName: Full=Protein associated with the ER translocon of 10kDa {ECO:0000250|UniProtKB:Q9Y284};
DE Short=PAT-10 {ECO:0000250|UniProtKB:Q9Y284};
DE Short=PAT10 {ECO:0000250|UniProtKB:Q9Y284};
GN Name=Wdr83os {ECO:0000312|MGI:MGI:3041257};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the PAT complex, an endoplasmic reticulum (ER)-
CC resident membrane multiprotein complex that facilitates multi-pass
CC membrane proteins insertion into membranes. The PAT complex acts as an
CC intramembrane chaperone by directly interacting with nascent
CC transmembrane domains (TMDs), releasing its substrates upon correct
CC folding, and is needed for optimal biogenesis of multi-pass membrane
CC proteins. WDR83OS/Asterix is the substrate-interacting subunit of the
CC PAT complex, whereas CCDC47 is required to maintain the stability of
CC WDR83OS/Asterix. WDR83OS/Asterix associates with the first
CC transmembrane domain (TMD1) of the nascent chain, independently of the
CC N-glycosylation of the chain and irrespective of the amino acid
CC sequence and transmembrane topology of TMD1. The PAT complex favors the
CC binding to TMDs with exposed hydrophilic amino acids within the lipid
CC bilayer and provides a membrane-embedded partially hydrophilic
CC environment in which TMD1 binds. {ECO:0000250|UniProtKB:Q9Y284}.
CC -!- SUBUNIT: The PAT complex includes WDR83OS/Asterix and CCDC47.
CC {ECO:0000250|UniProtKB:Q9Y284}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9Y284}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9Y284}.
CC -!- SIMILARITY: Belongs to the Asterix family. {ECO:0000305}.
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DR EMBL; AK010893; BAB27250.1; -; mRNA.
DR EMBL; BC056474; AAH56474.1; -; mRNA.
DR EMBL; BC089623; AAH89623.1; -; mRNA.
DR CCDS; CCDS80909.1; -.
DR RefSeq; NP_001001493.1; NM_001001493.2.
DR AlphaFoldDB; Q6ZWX0; -.
DR STRING; 10090.ENSMUSP00000078697; -.
DR PhosphoSitePlus; Q6ZWX0; -.
DR MaxQB; Q6ZWX0; -.
DR PaxDb; Q6ZWX0; -.
DR PRIDE; Q6ZWX0; -.
DR Antibodypedia; 26085; 32 antibodies from 14 providers.
DR DNASU; 414077; -.
DR Ensembl; ENSMUST00000079764; ENSMUSP00000078697; ENSMUSG00000059355.
DR GeneID; 414077; -.
DR KEGG; mmu:414077; -.
DR UCSC; uc009mpj.1; mouse.
DR CTD; 51398; -.
DR MGI; MGI:3041257; Wdr83os.
DR VEuPathDB; HostDB:ENSMUSG00000059355; -.
DR eggNOG; KOG3462; Eukaryota.
DR GeneTree; ENSGT00390000002121; -.
DR HOGENOM; CLU_128526_1_1_1; -.
DR InParanoid; Q6ZWX0; -.
DR OMA; CGLMIRM; -.
DR OrthoDB; 1608233at2759; -.
DR BioGRID-ORCS; 414077; 6 hits in 51 CRISPR screens.
DR ChiTaRS; Wdr83os; mouse.
DR PRO; PR:Q6ZWX0; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q6ZWX0; protein.
DR Bgee; ENSMUSG00000059355; Expressed in urinary bladder and 65 other tissues.
DR ExpressionAtlas; Q6ZWX0; baseline and differential.
DR Genevisible; Q6ZWX0; MM.
DR GO; GO:0101031; C:chaperone complex; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:MGI.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0044183; F:protein folding chaperone; ISS:UniProtKB.
DR GO; GO:0045048; P:protein insertion into ER membrane; ISS:UniProtKB.
DR InterPro; IPR005351; ASTER.
DR PANTHER; PTHR13193; PTHR13193; 1.
DR Pfam; PF03669; UPF0139; 1.
PE 3: Inferred from homology;
KW Acetylation; Chaperone; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9Y284"
FT CHAIN 2..106
FT /note="PAT complex subunit Asterix"
FT /id="PRO_0000071606"
FT TRANSMEM 40..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y284"
SQ SEQUENCE 106 AA; 12068 MW; 7D2BDDAF9279071B CRC64;
MSTNNMSDPR RPNKVLRYKP PPSECNPALD DPTPDYMNLL GMIFSMCGLM LKLKWCAWVA
VYCSFISFAN SRSSEDTKQM MSSFMLSISA VVMSYLQNPQ PMTPPW
