ID   PLH10_FORAG             Reviewed;         816 AA.
AC   T2KNA3;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Endo-acting ulvan lyase {ECO:0000303|PubMed:31285597};
DE            EC=4.2.2.- {ECO:0000269|PubMed:31285597};
DE   AltName: Full=Endolytic ulvan lyase;
DE   AltName: Full=P10_PLnc {ECO:0000303|PubMed:31285597};
DE   AltName: Full=Polysaccharide utilization locus H protein P10 {ECO:0000303|PubMed:31285597};
DE            Short=PUL H protein P10;
DE   Flags: Precursor;
GN   ORFNames=BN863_21990;
OS   Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 /
OS   M-2Alg 35-1).
OC   Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Formosa.
OX   NCBI_TaxID=1347342;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1;
RX   PubMed=23995932; DOI=10.1128/aem.01937-13;
RA   Mann A.J., Hahnke R.L., Huang S., Werner J., Xing P., Barbeyron T.,
RA   Huettel B., Stueber K., Reinhardt R., Harder J., Gloeckner F.O.,
RA   Amann R.I., Teeling H.;
RT   "The genome of the alga-associated marine flavobacterium Formosa agariphila
RT   KMM 3901T reveals a broad potential for degradation of algal
RT   polysaccharides.";
RL   Appl. Environ. Microbiol. 79:6813-6822(2013).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=31285597; DOI=10.1038/s41589-019-0311-9;
RA   Reisky L., Prechoux A., Zuehlke M.K., Baeumgen M., Robb C.S., Gerlach N.,
RA   Roret T., Stanetty C., Larocque R., Michel G., Song T., Markert S.,
RA   Unfried F., Mihovilovic M.D., Trautwein-Schult A., Becher D., Schweder T.,
RA   Bornscheuer U.T., Hehemann J.H.;
RT   "A marine bacterial enzymatic cascade degrades the algal polysaccharide
RT   ulvan.";
RL   Nat. Chem. Biol. 15:803-812(2019).
CC   -!- FUNCTION: Ulvan lyase involved in ulvan degradation (PubMed:31285597).
CC       Ulvan is the main polysaccharide component of the Ulvales (green
CC       seaweed) cell wall. It is composed of disaccharide building blocks
CC       comprising 3-sulfated rhamnose (Rha3S) linked to D-glucuronic acid
CC       (GlcA), L-iduronic acid (IduA), or D-xylose (Xyl) (Probable). Ulvan
CC       lyase catalyzes the endolytic cleavage of the glycosidic bond between
CC       Rha3S and the uronic acids GlcA or IduA, producing oligosaccharides
CC       that have unsaturated 4-deoxy-L-threo-hex-4-enopyranosiduronic acid
CC       (deltaUA) at the non-reducing end. This results eventually in the
CC       degradation of the ulvan polysaccharide into deltaUA-Rha3S
CC       disaccharides and deltaUA-Rha3S-Xyl-Rha3S tetrasaccharides
CC       (PubMed:31285597). {ECO:0000269|PubMed:31285597,
CC       ECO:0000305|PubMed:31285597}.
CC   -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000269|PubMed:31285597}.
CC       Periplasm {ECO:0000269|PubMed:31285597}.
CC   -!- INDUCTION: By ulvan and rhamnose. {ECO:0000269|PubMed:31285597}.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase family. {ECO:0000305}.
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DR   EMBL; HG315671; CDF79911.1; -; Genomic_DNA.
DR   RefSeq; WP_038530499.1; NZ_HG315671.1.
DR   AlphaFoldDB; T2KNA3; -.
DR   SMR; T2KNA3; -.
DR   STRING; 1347342.BN863_21990; -.
DR   EnsemblBacteria; CDF79911; CDF79911; BN863_21990.
DR   PATRIC; fig|1347342.6.peg.2206; -.
DR   eggNOG; ENOG502Z862; Bacteria.
DR   HOGENOM; CLU_014479_0_0_10; -.
DR   OrthoDB; 442785at2; -.
DR   Proteomes; UP000016160; Chromosome.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.100; -; 1.
DR   InterPro; IPR008929; Chondroitin_lyas.
DR   SUPFAM; SSF48230; SSF48230; 1.
PE   1: Evidence at protein level;
KW   Lyase; Periplasm; Reference proteome; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..816
FT                   /note="Endo-acting ulvan lyase"
FT                   /id="PRO_5004590961"
SQ   SEQUENCE   816 AA;  92871 MW;  DA8E9A102D00DE27 CRC64;
     MGTSVRRISV VLMMLFGTNF CWSQSMQHPV IWVTQDEKQD ILNLIEKYDW AKKMEHDLHA
     VVDKKVEAHQ KKPSVILSHI PEIPADNSLT EFEAVTVGDH AAVLTDASYA AMLYFLTDDE
     KYAQFSADVL WHYVTVLSDR SPKNTTICGN HFYDPRTSYA QFALAYDFIY NFLNKPTTKV
     YKASANKQQT FDRDLFQKVL LNMVGSSLQE YGRPDTHGKF ISNHPILTAP GVLYGILCIE
     DDKERERLFD VFWEKGTAHQ NSFKNTILPM FGKQGIWPES TSYSFMPAVT LVLNIIDRVY
     PEMQVTQNYK NIYKGNFLFD NLRMPDGRFV RYGDSKRNHD GTEQLYRYTL NLAQRRGYSN
     LENQAKIALS QAYQRQGGYQ SKISPATFNS SEPLKLFWGT PIPKGIDSKI DFKKPTVLVE
     HAGIALQRNY VETDNELYGL CGIIGGAHYV HSHVTGITME LYGAGYVMAP NGGLPKTVKE
     RRIPLHENYF RLYAGNNTVI VNGTSHGIQP GSWKDGAYVW QNTVVNIAAE PKHLEDPISE
     HFNFATQFLK DTINNCDQER TLSTIRTSEK TGYYLDVFRS KSLTENKFQD YIYHNIGDAT
     LLETENGETL RTEPTTRYKT DIGDPVQSPG WRYFEDTKST KPIHKGVHAT FKIDYDERFM
     HMFVPQGVNR SYTTALAPPT REAKNGYEEK PTQVLAIRQD GEAWEKPFIA VFEPSVKASS
     SVQTVTPLQD ADKVVGVTVI SKVNGKLITD YIISLDSKDG VYENKILKIK FEGRFGIIRV
     EGEQKTISLY IGEGKTLKYN NYTLDSETAT TAYKVF