PLH9_FORAG
ID PLH9_FORAG Reviewed; 353 AA.
AC T2KMF9;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 1.
DT 25-MAY-2022, entry version 23.
DE RecName: Full=6-phosphogluconolactonase {ECO:0000303|PubMed:31285597};
DE EC=3.1.1.31 {ECO:0000250|UniProtKB:O34499};
DE AltName: Full=P9_lactonase {ECO:0000303|PubMed:31285597};
DE AltName: Full=Polysaccharide utilization locus H protein P9 {ECO:0000303|PubMed:31285597};
DE Short=PUL H protein P9;
GN Name=pgl; ORFNames=BN863_21980;
OS Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 /
OS M-2Alg 35-1).
OC Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Formosa.
OX NCBI_TaxID=1347342;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1;
RX PubMed=23995932; DOI=10.1128/aem.01937-13;
RA Mann A.J., Hahnke R.L., Huang S., Werner J., Xing P., Barbeyron T.,
RA Huettel B., Stueber K., Reinhardt R., Harder J., Gloeckner F.O.,
RA Amann R.I., Teeling H.;
RT "The genome of the alga-associated marine flavobacterium Formosa agariphila
RT KMM 3901T reveals a broad potential for degradation of algal
RT polysaccharides.";
RL Appl. Environ. Microbiol. 79:6813-6822(2013).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=31285597; DOI=10.1038/s41589-019-0311-9;
RA Reisky L., Prechoux A., Zuehlke M.K., Baeumgen M., Robb C.S., Gerlach N.,
RA Roret T., Stanetty C., Larocque R., Michel G., Song T., Markert S.,
RA Unfried F., Mihovilovic M.D., Trautwein-Schult A., Becher D., Schweder T.,
RA Bornscheuer U.T., Hehemann J.H.;
RT "A marine bacterial enzymatic cascade degrades the algal polysaccharide
RT ulvan.";
RL Nat. Chem. Biol. 15:803-812(2019).
CC -!- FUNCTION: Carboxylic ester hydrolase that may be involved in ulvan
CC degradation (Probable). Ulvan is the main polysaccharide component of
CC the Ulvales (green seaweed) cell wall. It is composed of disaccharide
CC building blocks comprising 3-sulfated rhamnose (Rha3S) linked to D-
CC glucuronic acid (GlcA), L-iduronic acid (IduA), or D-xylose (Xyl)
CC (Probable). Catalyzes the hydrolysis of 6-phosphogluconolactone to 6-
CC phosphogluconate (By similarity). {ECO:0000250|UniProtKB:O34499,
CC ECO:0000305|PubMed:31285597}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate
CC + H(+); Xref=Rhea:RHEA:12556, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57955, ChEBI:CHEBI:58759; EC=3.1.1.31;
CC Evidence={ECO:0000250|UniProtKB:O34499};
CC -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-
CC ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step
CC 2/3. {ECO:0000250|UniProtKB:O34499}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:31285597}.
CC -!- INDUCTION: By ulvan and rhamnose. {ECO:0000269|PubMed:31285597}.
CC -!- SIMILARITY: Belongs to the cycloisomerase 2 family. {ECO:0000305}.
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DR EMBL; HG315671; CDF79910.1; -; Genomic_DNA.
DR AlphaFoldDB; T2KMF9; -.
DR SMR; T2KMF9; -.
DR STRING; 1347342.BN863_21980; -.
DR EnsemblBacteria; CDF79910; CDF79910; BN863_21980.
DR PATRIC; fig|1347342.6.peg.2205; -.
DR eggNOG; COG2706; Bacteria.
DR HOGENOM; CLU_038716_3_0_10; -.
DR OMA; NGPHAHM; -.
DR UniPathway; UPA00115; UER00409.
DR Proteomes; UP000016160; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0017057; F:6-phosphogluconolactonase activity; IEA:UniProtKB-EC.
DR GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR011048; Haem_d1_sf.
DR InterPro; IPR019405; Lactonase_7-beta_prop.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF10282; Lactonase; 1.
DR SUPFAM; SSF51004; SSF51004; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Glucose metabolism; Hydrolase;
KW Reference proteome.
FT CHAIN 1..353
FT /note="6-phosphogluconolactonase"
FT /id="PRO_0000448308"
SQ SEQUENCE 353 AA; 39035 MW; 06D6BF1DA37808EE CRC64;
MFVGSFTDKK PGTGIHVFDF NTKSGEAQLL SEVDSIINSS FLKLSPNGKY LYSVIESQLQ
THGKIAAFKI DSNAGDLKLI NMQDCGGRNP AHIEIDKSGK FLAVSNYTDP SLSFFEVDET
GKIKKIDEFF TFTGSGIVKG NQDTAHIHSS NFSLENDYLF LQDLGSDCIH KFKVNLDANQ
NMSLQKADAI KVKPGSGPRH FVFHQNGKYG YGINELSGKV SAYALLNGNL KFLADYNAYS
KKQDSYRSAD IHISPDGKFL YASNRGPNED SIVIFSINKS NGALKLIGHE PTYGEHPRNF
AIDPSGQFLL VANQFSNNIV IFRRDVETGK LQKLPQELVV NGSSSLQMFT YSH