PLI2B_ARATH
ID PLI2B_ARATH Reviewed; 205 AA.
AC Q1ECF5; Q9LQ78;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=LIM domain-containing protein PLIM2b {ECO:0000305};
DE AltName: Full=Pollen-expressed LIM protein 2B {ECO:0000305};
GN Name=PLIM2B {ECO:0000303|PubMed:17573466};
GN OrderedLocusNames=At1g01780 {ECO:0000312|Araport:AT1G01780};
GN ORFNames=T1N6.19 {ECO:0000312|EMBL:AAF78411.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABF83669.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17573466; DOI=10.1093/dnares/dsm013;
RA Arnaud D., Dejardin A., Leple J.C., Lesage-Descauses M.C., Pilate G.;
RT "Genome-wide analysis of LIM gene family in Populus trichocarpa,
RT Arabidopsis thaliana, and Oryza sativa.";
RL DNA Res. 14:103-116(2007).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP F-ACTIN.
RX PubMed=20817848; DOI=10.1105/tpc.110.075960;
RA Papuga J., Hoffmann C., Dieterle M., Moes D., Moreau F., Tholl S.,
RA Steinmetz A., Thomas C.;
RT "Arabidopsis LIM proteins: a family of actin bundlers with distinct
RT expression patterns and modes of regulation.";
RL Plant Cell 22:3034-3052(2010).
CC -!- FUNCTION: Binds to actin filaments and promotes cross-linking into
CC thick bundles. Has an actin-stabilizing activity. The actin regulatory
CC activities are inhibited by pH > 6.8 but are [Ca(2+)] independent.
CC {ECO:0000269|PubMed:20817848}.
CC -!- SUBUNIT: Interacts with F-actin. {ECO:0000269|PubMed:20817848}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:20817848}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in flowers and in pollen
CC grains. Detected in vasculature and roots.
CC {ECO:0000269|PubMed:20817848}.
CC -!- MISCELLANEOUS: Cross-links actin with a constant of dissociation of 1.7
CC uM. {ECO:0000269|PubMed:20817848}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF78411.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC009273; AAF78411.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE27334.1; -; Genomic_DNA.
DR EMBL; AK317367; BAH20039.1; -; mRNA.
DR EMBL; BT025779; ABF83669.1; -; mRNA.
DR PIR; D86149; D86149.
DR RefSeq; NP_171683.1; NM_100061.3.
DR AlphaFoldDB; Q1ECF5; -.
DR BioGRID; 24502; 10.
DR IntAct; Q1ECF5; 11.
DR STRING; 3702.AT1G01780.1; -.
DR iPTMnet; Q1ECF5; -.
DR PaxDb; Q1ECF5; -.
DR PRIDE; Q1ECF5; -.
DR ProteomicsDB; 234918; -.
DR EnsemblPlants; AT1G01780.1; AT1G01780.1; AT1G01780.
DR GeneID; 839267; -.
DR Gramene; AT1G01780.1; AT1G01780.1; AT1G01780.
DR KEGG; ath:AT1G01780; -.
DR Araport; AT1G01780; -.
DR TAIR; locus:2198210; AT1G01780.
DR eggNOG; KOG1700; Eukaryota.
DR HOGENOM; CLU_026811_1_0_1; -.
DR InParanoid; Q1ECF5; -.
DR OMA; IQMEGEC; -.
DR OrthoDB; 1214165at2759; -.
DR PRO; PR:Q1ECF5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q1ECF5; baseline and differential.
DR Genevisible; Q1ECF5; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0051017; P:actin filament bundle assembly; IDA:TAIR.
DR InterPro; IPR045268; LIMA-like.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24206; PTHR24206; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00132; LIM; 2.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Actin-binding; Cytoplasm; Cytoskeleton; LIM domain; Metal-binding;
KW Reference proteome; Repeat; Zinc.
FT CHAIN 1..205
FT /note="LIM domain-containing protein PLIM2b"
FT /id="PRO_0000430596"
FT DOMAIN 8..68
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 102..162
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REGION 177..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 205 AA; 22989 MW; 4687089B0E5799BF CRC64;
MSFTGTLDKC NVCDKTVYVV DMLSIEGMPY HKSCFRCTHC KGTLQMSNYS SMDGVLYCKT
HFEQLFKESG NFSKNFQPGK TEKPELTRTP SKISSIFCGT QDKCAACEKT VYPLEKIQME
GECFHKTCFR CAHGGCTLTH SSYASLDSVL YCRHHFNQLF MEKGNYAHVL QAANHRRTAS
GNTLPPEPTE DVAVEAKEEN GVSES
