PLI2C_ARATH
ID PLI2C_ARATH Reviewed; 213 AA.
AC Q500W4; Q9M2E1;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=LIM domain-containing protein PLIM2c {ECO:0000305};
DE AltName: Full=Pollen-expressed LIM protein 2C {ECO:0000305};
GN Name=PLIM2C {ECO:0000303|PubMed:17573466};
GN OrderedLocusNames=At3g61230 {ECO:0000312|Araport:AT3G61230};
GN ORFNames=T20K12.130 {ECO:0000312|EMBL:CAB71053.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAY34164.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Shinn P., Chen H., Cheuk R., Kim C.J., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis TRANSCRIPTION FACTOR GENES.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17573466; DOI=10.1093/dnares/dsm013;
RA Arnaud D., Dejardin A., Leple J.C., Lesage-Descauses M.C., Pilate G.;
RT "Genome-wide analysis of LIM gene family in Populus trichocarpa,
RT Arabidopsis thaliana, and Oryza sativa.";
RL DNA Res. 14:103-116(2007).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH
RP F-ACTIN, AND DOMAIN.
RX PubMed=20817848; DOI=10.1105/tpc.110.075960;
RA Papuga J., Hoffmann C., Dieterle M., Moes D., Moreau F., Tholl S.,
RA Steinmetz A., Thomas C.;
RT "Arabidopsis LIM proteins: a family of actin bundlers with distinct
RT expression patterns and modes of regulation.";
RL Plant Cell 22:3034-3052(2010).
CC -!- FUNCTION: Binds to actin filaments and promotes cross-linking into
CC thick bundles. Has an actin-stabilizing activity. Associates
CC predominantly with long and dynamic actin bundles in the shank of
CC growing pollen tubes. The actin regulatory activities are inhibited by
CC pH > 6.8 and/or high [Ca(2+)]. {ECO:0000269|PubMed:20817848}.
CC -!- SUBUNIT: Interacts with F-actin. {ECO:0000269|PubMed:20817848}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:20817848}.
CC -!- TISSUE SPECIFICITY: Exclusively expressed in pollen grains.
CC {ECO:0000269|PubMed:20817848}.
CC -!- DOMAIN: The C-terminal domain (159-213) is required for the ability to
CC respond to pH and calcium variations. {ECO:0000269|PubMed:20817848}.
CC -!- MISCELLANEOUS: Cross-links actin with a constant of dissociation of 1.5
CC uM. {ECO:0000269|PubMed:20817848}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB71053.1; Type=Erroneous gene model prediction;
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DR EMBL; AL137898; CAB71053.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80176.1; -; Genomic_DNA.
DR EMBL; BT022103; AAY34164.1; -; mRNA.
DR EMBL; AB493659; BAH30497.1; -; mRNA.
DR PIR; T47915; T47915.
DR RefSeq; NP_191682.2; NM_115987.4.
DR AlphaFoldDB; Q500W4; -.
DR BioGRID; 10609; 2.
DR IntAct; Q500W4; 2.
DR STRING; 3702.AT3G61230.1; -.
DR PaxDb; Q500W4; -.
DR PRIDE; Q500W4; -.
DR ProteomicsDB; 234768; -.
DR EnsemblPlants; AT3G61230.1; AT3G61230.1; AT3G61230.
DR GeneID; 825295; -.
DR Gramene; AT3G61230.1; AT3G61230.1; AT3G61230.
DR KEGG; ath:AT3G61230; -.
DR Araport; AT3G61230; -.
DR TAIR; locus:2098871; AT3G61230.
DR eggNOG; KOG1700; Eukaryota.
DR HOGENOM; CLU_026811_1_0_1; -.
DR InParanoid; Q500W4; -.
DR OMA; GSDKCAR; -.
DR OrthoDB; 1214165at2759; -.
DR PhylomeDB; Q500W4; -.
DR PRO; PR:Q500W4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q500W4; baseline and differential.
DR Genevisible; Q500W4; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051017; P:actin filament bundle assembly; IDA:TAIR.
DR InterPro; IPR045268; LIMA-like.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24206; PTHR24206; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00132; LIM; 2.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Actin-binding; Cytoplasm; Cytoskeleton; LIM domain; Metal-binding;
KW Reference proteome; Repeat; Zinc.
FT CHAIN 1..213
FT /note="LIM domain-containing protein PLIM2c"
FT /id="PRO_0000430597"
FT DOMAIN 9..69
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 105..165
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REGION 177..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 180..195
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 213 AA; 23765 MW; D0A9758DA0388BD6 CRC64;
MAAFTGTTDK CKACDKTVYV MDLMTLEGMP YHKSCFRCSH CNGTLVICNY SSMDGVLYCK
THFEQLFKES GNFSKNFQTA GKTEKSNDAT KAPNRLSSFF SGTQDKCAAC KKTVYPLEKM
TMEGESYHKT CFRCAHSGCP LTHSSYAALD GVLYCKVHFS QLFLEKGNYN HVLQAAANHR
RSTAEEDKTE PKEDEANPTE EETSDAAAEE HES
