PLIN4_HUMAN
ID PLIN4_HUMAN Reviewed; 1357 AA.
AC Q96Q06; A6NEI2;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Perilipin-4;
DE AltName: Full=Adipocyte protein S3-12;
GN Name=PLIN4; Synonyms=KIAA1881;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 76-1357 (ISOFORM 2), AND VARIANTS
RP ALA-124; GLY-659; ASN-761; ASN-860 AND THR-1124.
RC TISSUE=Brain;
RX PubMed=11572484; DOI=10.1093/dnares/8.4.179;
RA Nagase T., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XXI. The
RT complete sequences of 60 new cDNA clones from brain which code for large
RT proteins.";
RL DNA Res. 8:179-187(2001).
RN [3]
RP INDUCTION.
RX PubMed=15111493; DOI=10.2337/diabetes.53.5.1243;
RA Dalen K.T., Schoonjans K., Ulven S.M., Weedon-Fekjaer M.S., Bentzen T.G.,
RA Koutnikova H., Auwerx J., Nebb H.I.;
RT "Adipose tissue expression of the lipid droplet-associating proteins S3-12
RT and perilipin is controlled by peroxisome proliferator-activated receptor-
RT gamma.";
RL Diabetes 53:1243-1252(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: May play a role in triacylglycerol packaging into adipocytes.
CC May function as a coat protein involved in the biogenesis of lipid
CC droplets (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O88492}.
CC Cytoplasm {ECO:0000250|UniProtKB:O88492}. Lipid droplet
CC {ECO:0000250|UniProtKB:O88492}. Note=Nascent lipid droplet surface-
CC associated; association with lipid droplets is triacylglycerol
CC synthesis-dependent. {ECO:0000250|UniProtKB:O88492}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96Q06-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96Q06-2; Sequence=VSP_027278;
CC -!- INDUCTION: Up-regulated during adipocyte differentiation.
CC {ECO:0000269|PubMed:15111493}.
CC -!- SIMILARITY: Belongs to the perilipin family. {ECO:0000305}.
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DR EMBL; AC011498; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AB067468; BAB67774.1; -; mRNA.
DR RefSeq; NP_001073869.1; NM_001080400.1.
DR AlphaFoldDB; Q96Q06; -.
DR SMR; Q96Q06; -.
DR BioGRID; 609760; 4.
DR IntAct; Q96Q06; 3.
DR STRING; 9606.ENSP00000301286; -.
DR GlyConnect; 2883; 1 O-Linked glycan (1 site).
DR GlyGen; Q96Q06; 8 sites, 1 O-linked glycan (8 sites).
DR iPTMnet; Q96Q06; -.
DR PhosphoSitePlus; Q96Q06; -.
DR BioMuta; PLIN4; -.
DR DMDM; 156630940; -.
DR EPD; Q96Q06; -.
DR jPOST; Q96Q06; -.
DR MassIVE; Q96Q06; -.
DR MaxQB; Q96Q06; -.
DR PaxDb; Q96Q06; -.
DR PeptideAtlas; Q96Q06; -.
DR PRIDE; Q96Q06; -.
DR ProteomicsDB; 77803; -. [Q96Q06-1]
DR ProteomicsDB; 77804; -. [Q96Q06-2]
DR Antibodypedia; 23665; 120 antibodies from 30 providers.
DR CPTC; Q96Q06; 2 antibodies.
DR Ensembl; ENST00000301286.5; ENSP00000301286.4; ENSG00000167676.5.
DR UCSC; uc002mar.2; human. [Q96Q06-1]
DR GeneCards; PLIN4; -.
DR HGNC; HGNC:29393; PLIN4.
DR HPA; ENSG00000167676; Tissue enhanced (adipose tissue, breast, skeletal muscle).
DR MIM; 613247; gene.
DR neXtProt; NX_Q96Q06; -.
DR PharmGKB; PA165394042; -.
DR VEuPathDB; HostDB:ENSG00000167676; -.
DR eggNOG; KOG4744; Eukaryota.
DR InParanoid; Q96Q06; -.
DR OrthoDB; 71918at2759; -.
DR PhylomeDB; Q96Q06; -.
DR TreeFam; TF328397; -.
DR PathwayCommons; Q96Q06; -.
DR SignaLink; Q96Q06; -.
DR BioGRID-ORCS; 729359; 10 hits in 1071 CRISPR screens.
DR ChiTaRS; PLIN4; human.
DR GenomeRNAi; 729359; -.
DR Pharos; Q96Q06; Tbio.
DR PRO; PR:Q96Q06; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q96Q06; protein.
DR Bgee; ENSG00000167676; Expressed in subcutaneous adipose tissue and 154 other tissues.
DR ExpressionAtlas; Q96Q06; baseline and differential.
DR Genevisible; Q96Q06; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005811; C:lipid droplet; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR InterPro; IPR004279; Perilipin.
DR Pfam; PF03036; Perilipin; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Lipid droplet; Membrane;
KW Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1357
FT /note="Perilipin-4"
FT /id="PRO_0000297559"
FT REPEAT 95..127
FT /note="1"
FT REPEAT 128..160
FT /note="2"
FT REPEAT 161..193
FT /note="3"
FT REPEAT 194..226
FT /note="4"
FT REPEAT 227..259
FT /note="5"
FT REPEAT 260..292
FT /note="6"
FT REPEAT 293..325
FT /note="7"
FT REPEAT 326..358
FT /note="8"
FT REPEAT 359..391
FT /note="9"
FT REPEAT 392..424
FT /note="10"
FT REPEAT 425..457
FT /note="11"
FT REPEAT 458..490
FT /note="12"
FT REPEAT 491..523
FT /note="13"
FT REPEAT 524..556
FT /note="14"
FT REPEAT 557..589
FT /note="15"
FT REPEAT 590..622
FT /note="16"
FT REPEAT 623..655
FT /note="17"
FT REPEAT 656..688
FT /note="18"
FT REPEAT 689..721
FT /note="19"
FT REPEAT 722..754
FT /note="20"
FT REPEAT 755..787
FT /note="21"
FT REPEAT 788..820
FT /note="22"
FT REPEAT 821..853
FT /note="23"
FT REPEAT 854..886
FT /note="24"
FT REPEAT 887..919
FT /note="25"
FT REPEAT 920..952
FT /note="26"
FT REPEAT 953..985
FT /note="27"
FT REGION 27..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 95..985
FT /note="27 X 33 AA approximate tandem repeat"
FT REGION 1046..1079
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1226..1246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1046..1076
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88492"
FT MOD_RES 16
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88492"
FT VAR_SEQ 720
FT /note="C -> CSGVTGAANVAKGAVQTGVDTAKTVLTGTKDTVCSGVTGAVNVAKGA
FT VQTGLKTTQNIATGTKNTLG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11572484"
FT /id="VSP_027278"
FT VARIANT 124
FT /note="V -> A (in dbSNP:rs4807597)"
FT /evidence="ECO:0000269|PubMed:11572484"
FT /id="VAR_061507"
FT VARIANT 421
FT /note="T -> A (in dbSNP:rs55901236)"
FT /id="VAR_061508"
FT VARIANT 659
FT /note="S -> G (in dbSNP:rs7260518)"
FT /evidence="ECO:0000269|PubMed:11572484"
FT /id="VAR_050483"
FT VARIANT 761
FT /note="K -> N (in dbSNP:rs7256387)"
FT /evidence="ECO:0000269|PubMed:11572484"
FT /id="VAR_061509"
FT VARIANT 802
FT /note="M -> T (in dbSNP:rs7256712)"
FT /id="VAR_061510"
FT VARIANT 826
FT /note="A -> E (in dbSNP:rs7255187)"
FT /id="VAR_061511"
FT VARIANT 826
FT /note="A -> G (in dbSNP:rs7255187)"
FT /id="VAR_061512"
FT VARIANT 826
FT /note="A -> V (in dbSNP:rs7255187)"
FT /id="VAR_061513"
FT VARIANT 860
FT /note="K -> N (in dbSNP:rs7259721)"
FT /evidence="ECO:0000269|PubMed:11572484"
FT /id="VAR_061514"
FT VARIANT 1124
FT /note="A -> T (in dbSNP:rs7251858)"
FT /evidence="ECO:0000269|PubMed:11572484"
FT /id="VAR_034637"
FT VARIANT 1134
FT /note="R -> C (in dbSNP:rs7250947)"
FT /id="VAR_050484"
FT CONFLICT 263
FT /note="G -> S (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 668
FT /note="A -> V (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 734
FT /note="I -> V (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 819..826
FT /note="CSGVTGAA -> TTGLMGAV (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 832
FT /note="A -> T (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 836..844
FT /note="GLKTTQNIA -> SVDTTKTVL (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 849..852
FT /note="NTLG -> DTVC (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
FT CONFLICT 883
FT /note="A -> T (in Ref. 2; BAB67774)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1357 AA; 134431 MW; 24CCB9D3DD6B480D CRC64;
MQTLGSFFGS LPGFSSARNL VANAHSSARA RPAADPTGAP AAEAAQPQAQ VAAHPEQTAP
WTEKELQPSE KQMVSGAKDL VCSKMSRAKD AVSSGVASVV DVAKGVVQGG LDTTRSALTG
TKEVVSSGVT GAMDMAKGAV QGGLDTSKAV LTGTKDTVST GLTGAVNVAK GTVQAGVDTT
KTVLTGTKDT VTTGVMGAVN LAKGTVQTGV ETSKAVLTGT KDAVSTGLTG AVNVARGSIQ
TGVDTSKTVL TGTKDTVCSG VTGAMNVAKG TIQTGVDTSK TVLTGTKDTV CSGVTGAMNV
AKGTIQTGVD TSKTVLTGTK DTVCSGVTGA MNVAKGTIQT GVDTTKTVLT GTKNTVCSGV
TGAVNLAKEA IQGGLDTTKS MVMGTKDTMS TGLTGAANVA KGAMQTGLNT TQNIATGTKD
TVCSGVTGAM NLARGTIQTG VDTTKIVLTG TKDTVCSGVT GAANVAKGAV QGGLDTTKSV
LTGTKDAVST GLTGAVNVAK GTVQTGVDTT KTVLTGTKDT VCSGVTSAVN VAKGAVQGGL
DTTKSVVIGT KDTMSTGLTG AANVAKGAVQ TGVDTAKTVL TGTKDTVTTG LVGAVNVAKG
TVQTGMDTTK TVLTGTKDTI YSGVTSAVNV AKGAVQTGLK TTQNIATGTK NTFGSGVTSA
VNVAKGAAQT GVDTAKTVLT GTKDTVTTGL MGAVNVAKGT VQTSVDTTKT VLTGTKDTVC
SGVTGAANVA KGAIQGGLDT TKSVLTGTKD AVSTGLTGAV KLAKGTVQTG MDTTKTVLTG
TKDAVCSGVT GAANVAKGAV QMGVDTAKTV LTGTKDTVCS GVTGAANVAK GAVQTGLKTT
QNIATGTKNT LGSGVTGAAK VAKGAVQGGL DTTKSVLTGT KDAVSTGLTG AVNLAKGTVQ
TGVDTSKTVL TGTKDTVCSG VTGAVNVAKG TVQTGVDTAK TVLSGAKDAV TTGVTGAVNV
AKGTVQTGVD ASKAVLMGTK DTVFSGVTGA MSMAKGAVQG GLDTTKTVLT GTKDAVSAGL
MGSGNVATGA THTGLSTFQN WLPSTPATSW GGLTSSRTTD NGGEQTALSP QEAPFSGIST
PPDVLSVGPE PAWEAAATTK GLATDVATFT QGAAPGREDT GLLATTHGPE EAPRLAMLQN
ELEGLGDIFH PMNAEEQAQL AASQPGPKVL SAEQGSYFVR LGDLGPSFRQ RAFEHAVSHL
QHGQFQARDT LAQLQDCFRL IEKAQQAPEG QPRLDQGSGA SAEDAAVQEE RDAGVLSRVC
GLLRQLHTAY SGLVSSLQGL PAELQQPVGR ARHSLCELYG IVASAGSVEE LPAERLVQSR
EGVHQAWQGL EQLLEGLQHN PPLSWLVGPF ALPAGGQ
