PLK1_PEITU
ID PLK1_PEITU Reviewed; 34 AA.
AC P58606;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Toxin Ptu1 {ECO:0000303|PubMed:11423127};
OS Peirates turpis (Assassin bug).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Hemiptera; Heteroptera; Panheteroptera;
OC Cimicomorpha; Reduviidae; Peiratinae; Peirates.
OX NCBI_TaxID=181095;
RN [1]
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, SYNTHESIS, AND SUBCELLULAR LOCATION.
RC TISSUE=Saliva;
RX PubMed=11423127; DOI=10.1016/s0014-5793(01)02558-3;
RA Corzo G., Adachi-Akahane S., Nagao T., Kusui Y., Nakajima T.;
RT "Novel peptides from assassin bugs (Hemiptera: Reduviidae): isolation,
RT chemical and biological characterization.";
RL FEBS Lett. 499:256-261(2001).
RN [2]
RP STRUCTURE BY NMR, AND DISULFIDE BONDS.
RX PubMed=11669615; DOI=10.1021/bi015537j;
RA Bernard C., Corzo G., Mosbah A., Nakajima T., Darbon H.;
RT "Solution structure of Ptu1, a toxin from the assassin bug Peirates turpis
RT that blocks the voltage-sensitive calcium channel N-type.";
RL Biochemistry 40:12795-12800(2001).
CC -!- FUNCTION: Binds reversibly and blocks N-type voltage-gated calcium
CC channels (Cav). {ECO:0000250|UniProtKB:P58608}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11423127}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=3615.1; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:11423127};
CC -!- SIMILARITY: Belongs to the venom Ptu1-like knottin family.
CC {ECO:0000305}.
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DR PDB; 1I26; NMR; -; A=1-34.
DR PDBsum; 1I26; -.
DR AlphaFoldDB; P58606; -.
DR BMRB; P58606; -.
DR SMR; P58606; -.
DR EvolutionaryTrace; P58606; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019855; F:calcium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR012325; Assassin_bug_toxin-like.
DR Pfam; PF08117; Toxin_30; 1.
DR PROSITE; PS60010; ASSASSIN_BUG_TOXIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium channel impairing toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW Toxin; Voltage-gated calcium channel impairing toxin.
FT PEPTIDE 1..34
FT /note="Toxin Ptu1"
FT /evidence="ECO:0000269|PubMed:11423127"
FT /id="PRO_0000044891"
FT DISULFID 5..20
FT /evidence="ECO:0000269|PubMed:11669615,
FT ECO:0007744|PDB:1I26"
FT DISULFID 12..26
FT /evidence="ECO:0000269|PubMed:11669615,
FT ECO:0007744|PDB:1I26"
FT DISULFID 19..33
FT /evidence="ECO:0000269|PubMed:11669615,
FT ECO:0007744|PDB:1I26"
FT STRAND 4..6
FT /evidence="ECO:0007829|PDB:1I26"
FT TURN 13..16
FT /evidence="ECO:0007829|PDB:1I26"
FT STRAND 19..21
FT /evidence="ECO:0007829|PDB:1I26"
FT TURN 28..31
FT /evidence="ECO:0007829|PDB:1I26"
SQ SEQUENCE 34 AA; 3621 MW; 6B58A20A9E6B6FFA CRC64;
AEKDCIAPGA PCFGTDKPCC NPRAWCSSYA NKCL