PLLA_PHOLL
ID PLLA_PHOLL Reviewed; 122 AA.
AC Q7N561;
DT 31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Lectin A {ECO:0000303|PubMed:28972138};
DE AltName: Full=Alpha-galactoside-binding lectin {ECO:0000305|PubMed:28972138};
DE AltName: Full=Galactose-binding lectin PllA {ECO:0000303|PubMed:28972138};
GN Name=pllA {ECO:0000303|PubMed:28972138};
GN OrderedLocusNames=plu2096 {ECO:0000312|EMBL:CAE14389.1};
OS Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 /
OS TT01).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Morganellaceae; Photorhabdus.
OX NCBI_TaxID=243265;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15139 / CIP 105565 / TT01;
RX PubMed=14528314; DOI=10.1038/nbt886;
RA Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C.,
RA Lanois A., Powell K., Siguier P., Vincent R., Wingate V., Zouine M.,
RA Glaser P., Boemare N., Danchin A., Kunst F.;
RT "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT luminescens.";
RL Nat. Biotechnol. 21:1307-1313(2003).
RN [2] {ECO:0007744|PDB:5ODU, ECO:0007744|PDB:5OFI, ECO:0007744|PDB:5OFX, ECO:0007744|PDB:5OFZ}
RP X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) OF APOPROTEIN AND IN COMPLEX WITH
RP VARIOUS CARBOHYDRATES, FUNCTION, CARBOHYDRATE SPECIFICITY, BIOTECHNOLOGY,
RP AND SUBUNIT.
RC STRAIN=DSM 15139 / CIP 105565 / TT01;
RX PubMed=28972138; DOI=10.1074/jbc.m117.812792;
RA Beshr G., Sikandar A., Jemiller E.M., Klymiuk N., Hauck D., Wagner S.,
RA Wolf E., Koehnke J., Titz A.;
RT "Photorhabdus luminescens lectin A (PllA) - a new probe for detecting
RT alpha-galactoside-terminating glycoconjugates.";
RL J. Biol. Chem. 292:19935-19951(2017).
CC -!- FUNCTION: Lectin that specifically binds alpha-galactoside-terminating
CC glycoconjugates. Shows high apparent binding to the alpha-Gal epitope
CC (Gal-alpha-1,3-Gal-beta-1,4-GlcNAc terminating glycans) as well as to
CC Gal-alpha-1,4-GlcNAc and Gal-alpha-1,3-GalNAc. Gal-alpha-1,3-GalNAc may
CC be one natural ligand bound by PllA both in the nematode symbiont and
CC in infected insects. {ECO:0000269|PubMed:28972138}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:28972138}.
CC -!- BIOTECHNOLOGY: Can be used as a fluorescent probe for the specific
CC detection and visualization of the alpha-Gal epitope present on porcine
CC tissues. This epitope is mainly responsible for hyperacute rejection of
CC porcine organ transplants in humans during xenotransplantation.
CC {ECO:0000269|PubMed:28972138}.
CC -!- SIMILARITY: Belongs to the LecA/PllA lectin family. {ECO:0000305}.
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DR EMBL; BX571866; CAE14389.1; -; Genomic_DNA.
DR RefSeq; WP_011146351.1; NC_005126.1.
DR PDB; 5ODU; X-ray; 1.56 A; A/B/C/D/E/F/G/H=1-122.
DR PDB; 5OFI; X-ray; 2.00 A; A/B/C/D/E/F/G/H=1-122.
DR PDB; 5OFX; X-ray; 1.75 A; A/B/C/D/E/F/G/H=1-122.
DR PDB; 5OFZ; X-ray; 1.75 A; A/B/C/D=1-122.
DR PDBsum; 5ODU; -.
DR PDBsum; 5OFI; -.
DR PDBsum; 5OFX; -.
DR PDBsum; 5OFZ; -.
DR AlphaFoldDB; Q7N561; -.
DR SMR; Q7N561; -.
DR STRING; 243265.plu2096; -.
DR UniLectin; Q7N561; -.
DR EnsemblBacteria; CAE14389; CAE14389; plu2096.
DR GeneID; 24166541; -.
DR KEGG; plu:plu2096; -.
DR eggNOG; ENOG50330FC; Bacteria.
DR HOGENOM; CLU_162760_0_0_6; -.
DR OMA; KIESRYD; -.
DR OrthoDB; 1831069at2; -.
DR BioCyc; PLUM243265:PLU_RS10475-MON; -.
DR Proteomes; UP000002514; Chromosome.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR012905; PA-IL.
DR Pfam; PF07828; PA-IL; 1.
DR PIRSF; PIRSF020485; PA-IL; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Lectin; Metal-binding; Reference proteome.
FT CHAIN 1..122
FT /note="Lectin A"
FT /id="PRO_0000443107"
FT BINDING 38
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 44
FT /ligand="an alpha-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:46953"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 57
FT /ligand="an alpha-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:46953"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 96
FT /ligand="an alpha-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:46953"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 96
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 100
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 103
FT /ligand="an alpha-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:46953"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 103
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT BINDING 104
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000269|PubMed:28972138,
FT ECO:0007744|PDB:5ODU"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 16..22
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 27..33
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 36..41
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:5ODU"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 63..68
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 71..74
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 88..94
FT /evidence="ECO:0007829|PDB:5ODU"
FT HELIX 101..103
FT /evidence="ECO:0007829|PDB:5ODU"
FT STRAND 105..116
FT /evidence="ECO:0007829|PDB:5ODU"
SQ SEQUENCE 122 AA; 12958 MW; E2DF54B97D8F1461 CRC64;
MSDWSGSVPA NAENGKSTGL ILKQGDTISV VAHGWVKYGR DNVEWAAPDG PVPNNPQPSS
IATLVAKIAN KKFAIGNGVL HKTVPVDGEL ILLFNDVPGT FGDNSGEFQV EVIIESRYSP
LK
