PLM7_PLABA
ID PLM7_PLABA Reviewed; 471 AA.
AC A0A509ALH1;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2019, sequence version 1.
DT 03-AUG-2022, entry version 11.
DE RecName: Full=Plasmepsin VII {ECO:0000303|PubMed:24893340};
DE EC=3.4.23.- {ECO:0000305};
DE AltName: Full=Plasmepsin 7 {ECO:0000305};
DE Flags: Precursor;
GN Name=PMVII {ECO:0000303|PubMed:24893340};
GN ORFNames=PBANKA_0517600 {ECO:0000312|EMBL:VUC54589.1};
OS Plasmodium berghei (strain Anka).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX NCBI_TaxID=5823 {ECO:0000312|Proteomes:UP000074855};
RN [1] {ECO:0000312|Proteomes:UP000074855}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ANKA {ECO:0000312|Proteomes:UP000074855};
RX PubMed=25359557; DOI=10.1186/s12915-014-0086-0;
RA Otto T.D., Bohme U., Jackson A.P., Hunt M., Franke-Fayard B.,
RA Hoeijmakers W.A., Religa A.A., Robertson L., Sanders M., Ogun S.A.,
RA Cunningham D., Erhart A., Billker O., Khan S.M., Stunnenberg H.G.,
RA Langhorne J., Holder A.A., Waters A.P., Newbold C.I., Pain A., Berriman M.,
RA Janse C.J.;
RT "A comprehensive evaluation of rodent malaria parasite genomes and gene
RT expression.";
RL BMC Biol. 12:86-86(2014).
RN [2] {ECO:0000305}
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=24893340; DOI=10.1016/j.molbiopara.2014.05.004;
RA Mastan B.S., Kumari A., Gupta D., Mishra S., Kumar K.A.;
RT "Gene disruption reveals a dispensable role for plasmepsin VII in the
RT Plasmodium berghei life cycle.";
RL Mol. Biochem. Parasitol. 195:10-13(2014).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:A0A2I0BRF1}.
CC -!- DEVELOPMENTAL STAGE: During the mosquito stage, specifically expressed
CC at day 4 post-infection (PubMed:24893340). Not expressed in sporozoites
CC in the mosquito salivary glands and during the liver stage in the mouse
CC host (PubMed:24893340). {ECO:0000269|PubMed:24893340}.
CC -!- DISRUPTION PHENOTYPE: Normal blood stage development in the mouse host
CC (PubMed:24893340). In the mosquito, development of oocysts, and
CC sporozoite gliding motility and invasion of salivary glands are normal
CC (PubMed:24893340). {ECO:0000269|PubMed:24893340}.
CC -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
CC -!- CAUTION: It is unclear if PMVII is glycosylated as other members of the
CC same enzyme family, i.e. PMI and PMII, are not. {ECO:0000305}.
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DR EMBL; LK023120; VUC54589.1; -; Genomic_DNA.
DR SMR; A0A509ALH1; -.
DR STRING; 5823.A0A509ALH1; -.
DR VEuPathDB; PlasmoDB:PBANKA_0517600; -.
DR OMA; ENYMDME; -.
DR Proteomes; UP000074855; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd05471; pepsin_like; 1.
DR Gene3D; 2.40.70.10; -; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR034164; Pepsin-like_dom.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR PANTHER; PTHR47966; PTHR47966; 1.
DR Pfam; PF00026; Asp; 1.
DR PRINTS; PR00792; PEPSIN.
DR SUPFAM; SSF50630; SSF50630; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 1.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
PE 2: Evidence at transcript level;
KW Aspartyl protease; Cytoplasm; Hydrolase; Protease; Reference proteome;
KW Signal; Zymogen.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..?
FT /evidence="ECO:0000305"
FT /id="PRO_0000453696"
FT CHAIN ?..471
FT /note="Plasmepsin VII"
FT /evidence="ECO:0000255"
FT /id="PRO_5021436755"
FT DOMAIN 96..438
FT /note="Peptidase A1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT ACT_SITE 115
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT ACT_SITE 325
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
SQ SEQUENCE 471 AA; 54286 MW; 5544627A4C39E35B CRC64;
MKSVYHHFAI IFFLKLFLCN CILSIPKKTL GKGLFSLGLN EFKNNVDNNS LNILGELKNS
KPFINKSFIQ INEKKDNVLL LKLYKQNIAS DKLSTYYGKI AIGENSENIF NVLFDTGSTE
FWVPFKTCKF TKNNIHNKYE RTQSFKYKYD DKGLPSVLEI NYLSGKLVGF DGYDTVYLGP
GFAIPHTNIA FATSIDIPVL EKFKWDGIIG LGFENEDSQK RGIKPFLDHL KDEKILTEKN
YKNIFGYYIT NTGGYITLGG IDNRFKRSPD EKIIWSPVST EMGFWTIDIL GIRKEKQPYM
NERRDDEVIV KYEGFHDGSN KSIVDTGTFL IYAPKKTIEN YLNDLTINSC EDKQKLPYII
FQIKSKEIES IKGLSVIELV LSPNDYVIEY IDEVNSTKEC IIGIQSDEDN INGWTLGQVF
LKSYYTIFDK DNLQIGFVRN KQTINDETYL NESFLRVSKK RNKKKSYNGP L
