PLPL2_BOVIN
ID PLPL2_BOVIN Reviewed; 486 AA.
AC Q2KI18;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Patatin-like phospholipase domain-containing protein 2 {ECO:0000305};
DE EC=3.1.1.3 {ECO:0000250|UniProtKB:Q96AD5};
DE AltName: Full=Adipose triglyceride lipase;
DE AltName: Full=Calcium-independent phospholipase A2-zeta {ECO:0000250|UniProtKB:Q96AD5};
DE Short=iPLA2-zeta {ECO:0000250|UniProtKB:Q96AD5};
DE EC=3.1.1.4 {ECO:0000250|UniProtKB:Q96AD5};
GN Name=PNPLA2; Synonyms=ATGL;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Heart ventricle;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the initial step in triglyceride hydrolysis in
CC adipocyte and non-adipocyte lipid droplets (By similarity). Exhibits a
CC strong preference for the hydrolysis of long-chain fatty acid esters at
CC the sn-2 position of the glycerol backbone and acts coordinately with
CC LIPE/HLS and DGAT2 within the lipolytic cascade (By similarity). Also
CC possesses acylglycerol transacylase and phospholipase A2 activities (By
CC similarity). Transfers fatty acid from triglyceride to retinol,
CC hydrolyzes retinylesters, and generates 1,3-diacylglycerol from
CC triglycerides (By similarity). Regulates adiposome size and may be
CC involved in the degradation of adiposomes (By similarity). May play an
CC important role in energy homeostasis (By similarity). May play a role
CC in the response of the organism to starvation, enhancing hydrolysis of
CC triglycerides and providing free fatty acids to other tissues to be
CC oxidized in situations of energy depletion (By similarity).
CC {ECO:0000250|UniProtKB:Q8BJ56, ECO:0000250|UniProtKB:Q96AD5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacyl-sn-glycerol + H2O = a 1,2-diacylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:44864, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:49172,
CC ChEBI:CHEBI:64615; Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44865;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacyl-sn-glycerol + H2O = 1,3-diacyl-sn-glycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:43732, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:64615,
CC ChEBI:CHEBI:77272; Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43733;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacyl-sn-glycerol + H2O = a 2,3-diacyl-sn-glycerol + a
CC fatty acid + H(+); Xref=Rhea:RHEA:38499, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:64615,
CC ChEBI:CHEBI:75524; Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38500;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,3-diacylglycerol + a 1-acylglycerol = a triacylglycerol +
CC glycerol; Xref=Rhea:RHEA:44440, ChEBI:CHEBI:17754, ChEBI:CHEBI:17855,
CC ChEBI:CHEBI:35759, ChEBI:CHEBI:47777;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44441;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacylglycerol + a 1-acylglycerol = a triacylglycerol +
CC glycerol; Xref=Rhea:RHEA:44436, ChEBI:CHEBI:17754, ChEBI:CHEBI:17855,
CC ChEBI:CHEBI:35759, ChEBI:CHEBI:49172;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44437;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 a 1-acylglycerol = a 1,2-diacylglycerol + glycerol;
CC Xref=Rhea:RHEA:44432, ChEBI:CHEBI:17754, ChEBI:CHEBI:35759,
CC ChEBI:CHEBI:49172; Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44433;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacyl-sn-glycerol + all-trans-retinol = a diacylglycerol +
CC an all-trans-retinyl ester; Xref=Rhea:RHEA:44676, ChEBI:CHEBI:17336,
CC ChEBI:CHEBI:18035, ChEBI:CHEBI:63410, ChEBI:CHEBI:64615;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44677;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+)
CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823,
CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-
CC octadecenoate + 1,3-di-(9Z-octadecenoyl)-glycerol + H(+);
CC Xref=Rhea:RHEA:38387, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75735;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38388;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,3-di-(9Z-octadecenoyl)-glycerol + 1-(9Z-octadecenoyl)-
CC glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + glycerol;
CC Xref=Rhea:RHEA:38331, ChEBI:CHEBI:17754, ChEBI:CHEBI:53753,
CC ChEBI:CHEBI:75342, ChEBI:CHEBI:75735;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38332;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + 1-(9Z-octadecenoyl)-
CC glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + glycerol;
CC Xref=Rhea:RHEA:38327, ChEBI:CHEBI:17754, ChEBI:CHEBI:52323,
CC ChEBI:CHEBI:53753, ChEBI:CHEBI:75342;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38328;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-
CC glycerol + glycerol; Xref=Rhea:RHEA:38323, ChEBI:CHEBI:17754,
CC ChEBI:CHEBI:52323, ChEBI:CHEBI:75342;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38324;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + all-trans-retinol =
CC all-trans-retinyl 9Z-octadecenoate + di-(9Z)-octadecenoylglycerol;
CC Xref=Rhea:RHEA:39987, ChEBI:CHEBI:17336, ChEBI:CHEBI:53753,
CC ChEBI:CHEBI:70760, ChEBI:CHEBI:75945;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39988;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z)-hexadecenoylglycerol + H2O = (9Z)-hexadecenoate
CC + 1,3-di-(9Z)-hexadecenoylglycerol + H(+); Xref=Rhea:RHEA:38395,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372,
CC ChEBI:CHEBI:75841, ChEBI:CHEBI:75849;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38396;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z,12Z)-octadecadienoylglycerol + H2O = (9Z,12Z)-
CC octadecadienoate + 1,3-di-(9Z,12Z)-octadecadienoylglycerol + H(+);
CC Xref=Rhea:RHEA:38403, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30245, ChEBI:CHEBI:75844, ChEBI:CHEBI:75850;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38404;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z,12Z,15Z)-octadecatrienoylglycerol + H2O =
CC (9Z,12Z,15Z)-octadecatrienoate + 1,3-di-(9Z,12Z,15Z)-
CC octadecatrienoylglycerol + H(+); Xref=Rhea:RHEA:38411,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32387,
CC ChEBI:CHEBI:75845, ChEBI:CHEBI:75852;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38412;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,3-di-(9Z)-octadecenoyl-2-hexadecanoylglycerol + H2O = 1,3-
CC di-(9Z-octadecenoyl)-glycerol + H(+) + hexadecanoate;
CC Xref=Rhea:RHEA:38419, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:75735, ChEBI:CHEBI:75846;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38420;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + H2O =
CC (9Z)-octadecenoate + 1-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol +
CC H(+); Xref=Rhea:RHEA:38423, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:75583, ChEBI:CHEBI:75867;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38424;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O =
CC (9Z)-octadecenoate + 1-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol +
CC H(+); Xref=Rhea:RHEA:38647, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:75847, ChEBI:CHEBI:75868;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38648;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-
CC octadecenoate + 2,3-di-(9Z)-octadecenoyl-sn-glycerol + H(+);
CC Xref=Rhea:RHEA:38391, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75824;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38392;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z)-hexadecenoylglycerol + H2O = (9Z)-hexadecenoate
CC + 2,3-di-(9Z)-hexadecenoyl-sn-glycerol + H(+); Xref=Rhea:RHEA:38399,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372,
CC ChEBI:CHEBI:75841, ChEBI:CHEBI:75853;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38400;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z,12Z)-octadecadienoylglycerol + H2O = (9Z,12Z)-
CC octadecadienoate + 2,3-di-(9Z,12Z)-octadecadienoyl-sn-glycerol +
CC H(+); Xref=Rhea:RHEA:38407, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30245, ChEBI:CHEBI:75844, ChEBI:CHEBI:75854;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38408;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2,3-tri-(9Z,12Z,15Z)-octadecatrienoylglycerol + H2O =
CC (9Z,12Z,15Z)-octadecatrienoate + 2,3-di-(9Z,12Z,15Z)-
CC octadecatrienoyl-sn-glycerol + H(+); Xref=Rhea:RHEA:38415,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32387,
CC ChEBI:CHEBI:75845, ChEBI:CHEBI:75855;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38416;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,3-di-(9Z)-octadecenoyl-2-hexadecanoylglycerol + H2O = (9Z)-
CC octadecenoate + 2-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol +
CC H(+); Xref=Rhea:RHEA:38431, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:75846, ChEBI:CHEBI:75870;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38432;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O =
CC 2,3-di-(9Z)-octadecenoyl-sn-glycerol + H(+) + hexadecanoate;
CC Xref=Rhea:RHEA:38427, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:75824, ChEBI:CHEBI:75847;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38428;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + H2O =
CC (9Z)-octadecenoate + 2-(9Z-octadecenoyl)-3-hexadecanoyl-sn-glycerol +
CC H(+); Xref=Rhea:RHEA:38643, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:75546, ChEBI:CHEBI:75583;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38644;
CC Evidence={ECO:0000250|UniProtKB:Q8BJ56};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-
CC glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15802;
CC Evidence={ECO:0000250|UniProtKB:Q96AD5};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol degradation.
CC -!- SUBUNIT: Interacts with ABHD5; this association stimulates PNPLA2
CC triglyceride hydrolase activity. Interacts with SERPINF1; this
CC interaction stimulates the phospholipase A2 activity of PNPLA2. Despite
CC a colocalization in lipid droplets, it probably does not interact with
CC PLIN. Interacts with PLIN5; prevents interaction with ABHD5. Interacts
CC with FAF2. {ECO:0000250|UniProtKB:Q8BJ56,
CC ECO:0000250|UniProtKB:Q96AD5}.
CC -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q96AD5}.
CC Cell membrane {ECO:0000250|UniProtKB:Q96AD5}; Multi-pass membrane
CC protein {ECO:0000255}. Cytoplasm {ECO:0000250|UniProtKB:Q8BJ56}.
CC -!- PTM: Phosphorylation at Ser-409 by PKA is increased during fasting and
CC moderate intensity exercise, and moderately increases lipolytic
CC activity. {ECO:0000250|UniProtKB:Q8BJ56}.
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DR EMBL; BC112803; AAI12804.1; -; mRNA.
DR RefSeq; NP_001039470.1; NM_001046005.2.
DR AlphaFoldDB; Q2KI18; -.
DR STRING; 9913.ENSBTAP00000029866; -.
DR PaxDb; Q2KI18; -.
DR PRIDE; Q2KI18; -.
DR GeneID; 508493; -.
DR KEGG; bta:508493; -.
DR CTD; 57104; -.
DR eggNOG; KOG3773; Eukaryota.
DR InParanoid; Q2KI18; -.
DR OrthoDB; 1204225at2759; -.
DR BRENDA; 3.1.1.3; 908.
DR UniPathway; UPA00256; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051265; F:diolein transacylation activity; ISS:UniProtKB.
DR GO; GO:0051264; F:mono-olein transacylation activity; ISS:UniProtKB.
DR GO; GO:0004623; F:phospholipase A2 activity; ISS:UniProtKB.
DR GO; GO:0050253; F:retinyl-palmitate esterase activity; ISS:UniProtKB.
DR GO; GO:0004806; F:triglyceride lipase activity; ISS:UniProtKB.
DR GO; GO:0006651; P:diacylglycerol biosynthetic process; ISS:UniProtKB.
DR GO; GO:0010891; P:negative regulation of sequestering of triglyceride; ISS:UniProtKB.
DR GO; GO:0010898; P:positive regulation of triglyceride catabolic process; ISS:UniProtKB.
DR GO; GO:0019433; P:triglyceride catabolic process; ISS:UniProtKB.
DR CDD; cd07220; Pat_PNPLA2; 1.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR033562; PLPL.
DR InterPro; IPR033903; PNPLA2.
DR InterPro; IPR002641; PNPLA_dom.
DR PANTHER; PTHR12406; PTHR12406; 1.
DR Pfam; PF01734; Patatin; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR PROSITE; PS51635; PNPLA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasm; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid droplet; Lipid metabolism; Membrane; Phosphoprotein;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..486
FT /note="Patatin-like phospholipase domain-containing protein
FT 2"
FT /id="PRO_0000292526"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96AD5"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..42
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q96AD5"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96AD5"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..334
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q96AD5"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..486
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96AD5"
FT DOMAIN 10..179
FT /note="PNPLA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 14..19
FT /note="GXGXXG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 45..49
FT /note="GXSXG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 166..168
FT /note="DGA/G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT ACT_SITE 47
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOD_RES 377
FT /note="Phosphoserine; in vitro"
FT /evidence="ECO:0000250|UniProtKB:Q8BJ56"
FT MOD_RES 399
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000250|UniProtKB:Q8BJ56"
FT MOD_RES 409
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000250|UniProtKB:Q96AD5"
FT MOD_RES 433
FT /note="Phosphoserine; in vitro"
FT /evidence="ECO:0000250|UniProtKB:Q8BJ56"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 486 AA; 53417 MW; 31925E73C4B82ACD CRC64;
MFPKETTWNI SFAGCGFLGV YHIGVASCLR EHAPFLVANA THIYGASAGA LTATALVTGA
CLGEAGANII EVSKEARKRF LGPLHPSFNM VKTIRGCLLK ILPADCYECA SGRLGISLTR
VSDGENVIIT HFNSKEELIQ ANVCSTFIPV YCGLIPPSLQ GVRYVDGGIS DNLPLYELKN
TITVSPFSGE SDICPQDSST NIHELRVTNT SIQFNLRNLY RLSKALFPPE PLVLREMCKQ
GYRDGLRFLR RNGLLNRPNP LLALPPSQPP APEDADAQEG AVAMERTGGK DHLPPPREDH
ILEHLPSRLN EALLEACMEP TDLLTTLSNM LPVRLAMAMM VPYTLPLESA VSFTIRLLEW
LPDVPEDIRW MKEQTGSICQ YLMIRAKRKL GNHLPSRLSG QVVLRRARSL PSVPLSCAAY
SEVLPSWMRN SLSLGDVLAK WEECQRQLLL GLFCTNVAFP PDALRMRVPA GPAPEPPQHP
PSSPPC
