ID   PLPL_ASPOR              Reviewed;         717 AA.
AC   Q2UKE6;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Patatin-like phospholipase domain-containing protein AO090003000839;
DE            EC=3.1.1.-;
GN   ORFNames=AO090003000839;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Probable lipid hydrolase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PLPL family. {ECO:0000305}.
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DR   EMBL; AP007155; BAE57969.1; -; Genomic_DNA.
DR   RefSeq; XP_001819971.1; XM_001819919.2.
DR   AlphaFoldDB; Q2UKE6; -.
DR   STRING; 510516.Q2UKE6; -.
DR   PRIDE; Q2UKE6; -.
DR   EnsemblFungi; BAE57969; BAE57969; AO090003000839.
DR   GeneID; 5991954; -.
DR   KEGG; aor:AO090003000839; -.
DR   HOGENOM; CLU_009031_2_2_1; -.
DR   OMA; DMNKWLR; -.
DR   Proteomes; UP000006564; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005811; C:lipid droplet; IEA:EnsemblFungi.
DR   GO; GO:0004806; F:triglyceride lipase activity; IEA:EnsemblFungi.
DR   GO; GO:1990748; P:cellular detoxification; IEA:EnsemblFungi.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006642; P:triglyceride mobilization; IEA:EnsemblFungi.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR002641; PNPLA_dom.
DR   InterPro; IPR021771; Triacylglycerol_lipase_N.
DR   Pfam; PF11815; DUF3336; 1.
DR   Pfam; PF01734; Patatin; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS51635; PNPLA; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..717
FT                   /note="Patatin-like phospholipase domain-containing protein
FT                   AO090003000839"
FT                   /id="PRO_0000295552"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          277..468
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   REGION          620..696
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           308..312
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   COMPBIAS        630..660
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        661..686
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        310
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        455
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
SQ   SEQUENCE   717 AA;  82365 MW;  08B8EADCFA79FC63 CRC64;
     MNSPEKSAAC DIYDPKSIPD YDREFIDPDD LRQFENALND NESNSLVALN DWRPIYQRVR
     KNRGRRKKPR RTTDETREGV LYTVLKWPFL FIVFGWITVL GFAYALTRFY IVLYERWVSW
     RGKKESLRRE LWKQTDYNNW LKAAQALDNH LGNQQWKEID EYAYYDHLTI NKLVNQLRKA
     RTDVELQMRN GVSSSTVIPA TEELCALLEG CVKNNFAGVE NPRLYSETYS GTKNLVQEYI
     DEVEKCIQVV SNNKWVSNED KYHHFKHLDT NFGRTALCLS GGATFAYYHF GVARALLDNG
     VLPEIITGTS GGALVAALIA TRTDEELKQL LVPALAHRIR ASSEGMASWI WRWWRTGARF
     DTITWARECS WFCRGSTTFK EAYERTGRIL NVSCVPSDPH SPTILANYLT SPNCVIWSAV
     LASAAVPGIL NPVVLMTKKR DGTLAPYSFG HKWKDGSLRT DIPIRALNLH FNVNFPIVSQ
     VNPHINLFFF SSRGSVGRPV THRRGRGWRG GFLGSAIEQY IKLDLNKWLR VLRHLELLPR
     PLGQDWSEIW LQKFSGTITI WPKTIPSDFY YILSDPTPER LARMLNVGQQ SAFPMIQFIK
     NRLKIENAIL KGLHQYSPAV SPAQSRRKRG HAGKPSDPMV ERLDHNLPDR QPDNKEDLSD
     SSGIDSNVSS RDSCLQPSSN RRNRRRSTGN IFQEMRRQSA VFFDDSDLYA EDDKKVE