ID   PLPL_CRYNB              Reviewed;         871 AA.
AC   P0CP53; Q55SE8; Q5KGU2;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Patatin-like phospholipase domain-containing protein CNBE2340;
DE            EC=3.1.1.-;
GN   OrderedLocusNames=CNBE2340;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Probable lipid hydrolase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PLPL family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL20873.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AAEY01000024; EAL20873.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_775520.1; XM_770427.1.
DR   AlphaFoldDB; P0CP53; -.
DR   PRIDE; P0CP53; -.
DR   EnsemblFungi; EAL20873; EAL20873; CNBE2340.
DR   GeneID; 4936244; -.
DR   KEGG; cnb:CNBE2340; -.
DR   HOGENOM; CLU_009031_2_1_1; -.
DR   Proteomes; UP000001435; Chromosome 5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004806; F:triglyceride lipase activity; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006641; P:triglyceride metabolic process; IEA:UniProt.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR002641; PNPLA_dom.
DR   InterPro; IPR021771; Triacylglycerol_lipase_N.
DR   Pfam; PF11815; DUF3336; 1.
DR   Pfam; PF01734; Patatin; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS51635; PNPLA; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..871
FT                   /note="Patatin-like phospholipase domain-containing protein
FT                   CNBE2340"
FT                   /id="PRO_0000410184"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          243..435
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   REGION          20..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          586..707
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          720..748
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          760..871
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           274..278
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   COMPBIAS        20..47
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        590..605
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        623..639
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        689..707
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        722..748
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        777..798
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        799..825
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        276
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        422
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
SQ   SEQUENCE   871 AA;  96690 MW;  154E45142FF3F24B CRC64;
     MPPSPPSSAA ADQWNIDYVN EDSPLSPRSF SLPPESPQLS TASPIHQRVS RKRQRATSQG
     LTYNVIRWPL LFFIFFIIYL EFSAYVITRQ IVNVFEWLVA WRGYKAKLRK ELRKAKTYDE
     WVNTAKKLDK HLGFDDWKDV EEDSYFDWAL VRRVRRTLTR LRAANDTRGL MDALAVCVRA
     NFAGTESVKM YSETFIGTKK AVEAHIKEVA ACLDYVRTAT DVSLEEKRAF FRAVNKHYGS
     SALCLSGGAS FGYYHFGVIK AFLEADLLPR VITGTSAGGL CAALLCTRTD SELKELLVPE
     LADKITACSD PFTVWFKRFR QTGARFDTID WARRSMWFTR GSLTFKEAYT KTGRALNISV
     VPSDRHSPTI LLNHLTAPNC LIWSAILASA AVPGILNPVV LMAKDRSGNI KPHNLGGSRF
     KDGSLREDIP LGSLHTQFNC NFSIVSQTNP HIHLFFFAPR GSVGRPVAHR KGKGWRGGFI
     LSALESYIKL DLSKHFKVIR DLDLMPQILQ SDWSGVFLQR FSGDLTLTPR STIGDWFHIL
     SDPDRPQMKR MLRVGERVAW PALGMVRNRM TVERAILRGR SEVRTALSHD RTSNDPATSL
     PETNPELTGA LDHVPIESDV DAGFVSRSRR ARNKTGSKGG DTPEEQLNLA GVFQLDESSK
     GVRRRKQKKS GMPLVAEPLG ELPEVSPTHS PIATESPQRN YTSNFGDSFR HVRAPSLPAL
     SSPFRSIRSN TSSSSNNVQS PSSSQRFRSQ LSITRWFGGV SESSSDEEDE DLGGLQSGEA
     TASSGEEGIP TFQLDSAVES HSDRSEDEML HSGANVKEEY QSEESEGKIP IPGGERVTQE
     KIDASMASGE RLRPAGGSKG SAKTPPVQDG A