ID   PLPL_PICGU              Reviewed;         717 AA.
AC   A5DIR3;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Patatin-like phospholipase domain-containing protein PGUG_03164;
DE            EC=3.1.1.-;
GN   ORFNames=PGUG_03164;
OS   Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS   / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX   NCBI_TaxID=294746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX   PubMed=19465905; DOI=10.1038/nature08064;
RA   Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA   Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA   Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA   Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA   Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA   Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA   Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA   Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA   Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA   Birren B.W., Kellis M., Cuomo C.A.;
RT   "Evolution of pathogenicity and sexual reproduction in eight Candida
RT   genomes.";
RL   Nature 459:657-662(2009).
CC   -!- FUNCTION: Probable lipid hydrolase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PLPL family. {ECO:0000305}.
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DR   EMBL; CH408157; EDK39066.2; -; Genomic_DNA.
DR   RefSeq; XP_001485435.1; XM_001485385.1.
DR   AlphaFoldDB; A5DIR3; -.
DR   STRING; 4929.XP_001485435.1; -.
DR   EnsemblFungi; EDK39066; EDK39066; PGUG_03164.
DR   GeneID; 5127254; -.
DR   KEGG; pgu:PGUG_03164; -.
DR   VEuPathDB; FungiDB:PGUG_03164; -.
DR   eggNOG; KOG2214; Eukaryota.
DR   HOGENOM; CLU_009031_2_2_1; -.
DR   InParanoid; A5DIR3; -.
DR   OMA; DMNKWLR; -.
DR   OrthoDB; 425613at2759; -.
DR   Proteomes; UP000001997; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004806; F:triglyceride lipase activity; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006641; P:triglyceride metabolic process; IEA:UniProt.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR002641; PNPLA_dom.
DR   InterPro; IPR021771; Triacylglycerol_lipase_N.
DR   Pfam; PF11815; DUF3336; 1.
DR   Pfam; PF01734; Patatin; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS51635; PNPLA; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..717
FT                   /note="Patatin-like phospholipase domain-containing protein
FT                   PGUG_03164"
FT                   /id="PRO_0000295565"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          298..490
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   REGION          680..717
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           329..333
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   COMPBIAS        686..701
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        331
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        477
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
SQ   SEQUENCE   717 AA;  82107 MW;  77ACF54A9F37A233 CRC64;
     MTSSTPLYDE NEDYIDENHI NTFAKALLWE DDDHDGLASP PLPAYEGDKL AEVNAEELSS
     VLTPEPNNES EKSRPEFVVS HNDWYPINAP EKSKAKADAK GRKKRASTRD EFRSSAAYTL
     LRWPFLIIIT VWILLLCILY TVVRAYVALS EYMLTWVGER KVLRNKLRAS KTYEEWIENA
     LELDRYLHLD KWSSIPRFSY YDYRTVKRTT SKLRMLRMRG MDEELMVFLQ GCLKKNFAGI
     ENRQLYAHRY YGTKNVVHVY IDEVVASIDH VTESENITPE DKRRFFRSVS RNYGRTALCL
     SGGACFAYTH FGIVKALLDN DLLPSIITGT SGGGLVAALA CTRTDDELKQ LLVPRLARKI
     TACEDPWYVW IPRWWRTGAR FDSTAWARKS NYFTLGSLTF QEAYHRTGRR LNISTVPADP
     HSPVILCNNI TAPNCIIWSC LLASSAVPGI LNPVVLMMKD SKKNTIVPFS LGSKWKDGSL
     RTDIPIDALK TYYNVNFTVV SQVNPHISLF FFAPKGSVGR PVASSRRKTR REKYASLRGG
     FIATALEHLF KLEIKKWLEM IKTLDLLPRL SESDWSSIWL QRFTGSITIW PRNNFRDFWY
     ILSDPSEEGL GEMIRKGERY MFPKILFLKH RLSIENAIER GRTKSKLSTA HLEHSPRFSG
     TPEFAGPEFQ LQTVHYDDDY DSESSAEETL SPGFSQGTHA VLTDESDDDS SDDEIDD