PLRG1_MOUSE
ID PLRG1_MOUSE Reviewed; 513 AA.
AC Q922V4; O55039;
DT 07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Pleiotropic regulator 1;
GN Name=Plrg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Okresz L.;
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C3H/He; TISSUE=Mammary tumor, and Mesenchymal cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the
CC spliceosome. Component of the PRP19-CDC5L complex that forms an
CC integral part of the spliceosome and is required for activating pre-
CC mRNA splicing. {ECO:0000250|UniProtKB:O43660}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. Component of the
CC PRP19-CDC5L splicing complex composed of a core complex comprising a
CC homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less
CC stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its
CC WD40 repeat domain) directly with CDC5L (via its C-terminal); the
CC interaction is required for mRNA splicing but not for spliceosome
CC assembly. Also interacts directly in the complex with BCAS2 and PRPF19.
CC Interacts with USB1 (By similarity). {ECO:0000250|UniProtKB:O43660}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43660}. Nucleus
CC speckle {ECO:0000250|UniProtKB:O43660}.
CC -!- SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. {ECO:0000305}.
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DR EMBL; AF044334; AAC04388.1; -; mRNA.
DR EMBL; AK075993; BAC36104.1; -; mRNA.
DR EMBL; AK076007; BAC36115.1; -; mRNA.
DR EMBL; AK077197; BAC36675.1; -; mRNA.
DR EMBL; BC006750; AAH06750.1; -; mRNA.
DR EMBL; BC052895; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS17433.1; -.
DR RefSeq; NP_058064.2; NM_016784.3.
DR AlphaFoldDB; Q922V4; -.
DR SMR; Q922V4; -.
DR BioGRID; 207280; 39.
DR ComplexPortal; CPX-5825; PRP19-CDC5L complex.
DR IntAct; Q922V4; 3.
DR MINT; Q922V4; -.
DR STRING; 10090.ENSMUSP00000114968; -.
DR iPTMnet; Q922V4; -.
DR PhosphoSitePlus; Q922V4; -.
DR EPD; Q922V4; -.
DR MaxQB; Q922V4; -.
DR PaxDb; Q922V4; -.
DR PeptideAtlas; Q922V4; -.
DR PRIDE; Q922V4; -.
DR ProteomicsDB; 289548; -.
DR Antibodypedia; 16702; 256 antibodies from 30 providers.
DR DNASU; 53317; -.
DR Ensembl; ENSMUST00000150268; ENSMUSP00000114968; ENSMUSG00000027998.
DR GeneID; 53317; -.
DR KEGG; mmu:53317; -.
DR UCSC; uc008ppi.1; mouse.
DR CTD; 5356; -.
DR MGI; MGI:1858197; Plrg1.
DR VEuPathDB; HostDB:ENSMUSG00000027998; -.
DR eggNOG; KOG0285; Eukaryota.
DR GeneTree; ENSGT00940000155316; -.
DR InParanoid; Q922V4; -.
DR OMA; HPLEWKP; -.
DR OrthoDB; 1157847at2759; -.
DR PhylomeDB; Q922V4; -.
DR TreeFam; TF105684; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 53317; 26 hits in 73 CRISPR screens.
DR PRO; PR:Q922V4; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q922V4; protein.
DR Bgee; ENSMUSG00000027998; Expressed in primitive streak and 264 other tissues.
DR ExpressionAtlas; Q922V4; baseline and differential.
DR Genevisible; Q922V4; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0005662; C:DNA replication factor A complex; ISO:MGI.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000974; C:Prp19 complex; ISO:MGI.
DR GO; GO:0005681; C:spliceosomal complex; ISO:MGI.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; IMP:MGI.
DR GO; GO:0034504; P:protein localization to nucleus; IMP:MGI.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR045241; Prp46/PLRG1-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19923; PTHR19923; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Spliceosome; WD repeat.
FT CHAIN 1..513
FT /note="Pleiotropic regulator 1"
FT /id="PRO_0000051134"
FT REPEAT 201..240
FT /note="WD 1"
FT REPEAT 243..282
FT /note="WD 2"
FT REPEAT 285..324
FT /note="WD 3"
FT REPEAT 327..366
FT /note="WD 4"
FT REPEAT 369..409
FT /note="WD 5"
FT REPEAT 410..448
FT /note="WD 6"
FT REPEAT 459..498
FT /note="WD 7"
FT REGION 60..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT MOD_RES 119
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT MOD_RES 200
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT MOD_RES 390
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT CONFLICT 102
FT /note="A -> P (in Ref. 1; AAC04388)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 513 AA; 56938 MW; 8C9A2ED99D84ADEA CRC64;
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT
SKENLKEKGP QNATDSYPHK QYPANQGQDV EYLVTGTHPY PAGPGVALTA DTKIQRMPSE
SAAQSLAVAL PSQTRVDANR TGPAGSEYRH PGASDRSQPT AMNSIMETGN TKNSALMAKK
APTMPKPQWH PPWKLYRVIS GHLGWVRCIA VEPGNQWFVT GSADRTIKIW DLASGKLKLS
LTGHISTVRG VIVSTRSPYL FSCGEDKQVK CWDLEYNKVI RHYHGHLSAV YGLDLHPTLD
VLVTCSRDST ARIWDVRTKA SVHTLSGHTN AVATVRCQAA EPQIITGSHD TTIRLWDLVA
GKTRVTLTNH KKSVRAVVLH PLLYTFASGS PDNIKQWKFP DGGFIQNLSG HNAIINTLAV
NADGVLVSGA DNGTMHLWDW RTGYNFQRVH AAVQPGSLDS ESGIFACAFD RSESRLLTAE
ADKTIKVYRE DETATEETHP VSWKPEIIKR KRF
