PLRG1_RAT
ID PLRG1_RAT Reviewed; 514 AA.
AC Q9WUC8;
DT 07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Pleiotropic regulator 1;
GN Name=Plrg1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Okresz L.;
RL Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the
CC spliceosome. Component of the PRP19-CDC5L complex that forms an
CC integral part of the spliceosome and is required for activating pre-
CC mRNA splicing. {ECO:0000250|UniProtKB:O43660}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. Component of the
CC PRP19-CDC5L splicing complex composed of a core complex comprising a
CC homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less
CC stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its
CC WD40 repeat domain) directly with CDC5L (via its C-terminal); the
CC interaction is required for mRNA splicing but not for spliceosome
CC assembly. Also interacts directly in the complex with BCAS2 and PRPF19.
CC Interacts with USB1 (By similarity). {ECO:0000250|UniProtKB:O43660}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43660}. Nucleus
CC speckle {ECO:0000250|UniProtKB:O43660}.
CC -!- SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. {ECO:0000305}.
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DR EMBL; AF128241; AAD24799.1; -; mRNA.
DR EMBL; BC087742; AAH87742.1; -; mRNA.
DR RefSeq; NP_068525.1; NM_021757.2.
DR AlphaFoldDB; Q9WUC8; -.
DR SMR; Q9WUC8; -.
DR BioGRID; 248804; 1.
DR IntAct; Q9WUC8; 1.
DR STRING; 10116.ENSRNOP00000009112; -.
DR jPOST; Q9WUC8; -.
DR PaxDb; Q9WUC8; -.
DR PRIDE; Q9WUC8; -.
DR Ensembl; ENSRNOT00000009113; ENSRNOP00000009112; ENSRNOG00000006655.
DR GeneID; 60376; -.
DR KEGG; rno:60376; -.
DR UCSC; RGD:621047; rat.
DR CTD; 5356; -.
DR RGD; 621047; Plrg1.
DR eggNOG; KOG0285; Eukaryota.
DR GeneTree; ENSGT00940000155316; -.
DR HOGENOM; CLU_000288_72_2_1; -.
DR InParanoid; Q9WUC8; -.
DR OMA; HPLEWKP; -.
DR OrthoDB; 1157847at2759; -.
DR PhylomeDB; Q9WUC8; -.
DR TreeFam; TF105684; -.
DR Reactome; R-RNO-72163; mRNA Splicing - Major Pathway.
DR PRO; PR:Q9WUC8; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000006655; Expressed in thymus and 20 other tissues.
DR Genevisible; Q9WUC8; RN.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:RGD.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000974; C:Prp19 complex; ISO:RGD.
DR GO; GO:0005681; C:spliceosomal complex; ISO:RGD.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; ISO:RGD.
DR GO; GO:0034504; P:protein localization to nucleus; ISO:RGD.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR045241; Prp46/PLRG1-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19923; PTHR19923; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Spliceosome; WD repeat.
FT CHAIN 1..514
FT /note="Pleiotropic regulator 1"
FT /id="PRO_0000051135"
FT REPEAT 202..241
FT /note="WD 1"
FT REPEAT 244..283
FT /note="WD 2"
FT REPEAT 286..325
FT /note="WD 3"
FT REPEAT 328..367
FT /note="WD 4"
FT REPEAT 370..410
FT /note="WD 5"
FT REPEAT 411..449
FT /note="WD 6"
FT REPEAT 460..499
FT /note="WD 7"
FT REGION 56..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 140..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT MOD_RES 119
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT MOD_RES 201
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
FT MOD_RES 391
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43660"
SQ SEQUENCE 514 AA; 57189 MW; BAB644F1923CFDC9 CRC64;
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT
SKENLKEKGP QNAPDSYPHK QYPANQGQDV EYLVTGTHPY PPGPGVALTA DTKIQRMPSE
SAAQSLAVAL PSQTRVDANR TAPAGSEYRH PGASDRSQPT AMNSMIMETS NTKNSALMAK
KAPTMPKPQW HPPWKLYRVI SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL
SLTGHISTVR GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI
DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH DTTIRLWDLV
AGKTRVTLTN HKKSVRAVVL HPLHYTFASG SPDNIKQWKF PDGGFIQNLS GHNAIINTLA
VNTDGVLVSG ADNGTMHLWD WRTGYNFQRV HAAVQPGSLD SESGIFACAF DRSESRLLTA
EADKTIKVYR EDETATEETH PVSWKPEIIK RKRF
