PLRX4_ARATH
ID PLRX4_ARATH Reviewed; 699 AA.
AC O81765; Q1PE26;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Pollen-specific leucine-rich repeat extensin-like protein 4;
DE Short=AtPEX4;
DE Short=Pollen-specific LRR/EXTENSIN4;
DE AltName: Full=Cell wall hydroxyproline-rich glycoprotein;
DE Flags: Precursor;
GN Name=PEX4; OrderedLocusNames=At4g33970; ORFNames=F17I5.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644681; DOI=10.1104/pp.102.014928;
RA Baumberger N., Doesseger B., Guyot R., Diet A., Parsons R.L., Clark M.A.,
RA Simmons M.P., Bedinger P., Goff S.A., Ringli C., Keller B.;
RT "Whole-genome comparison of leucine-rich repeat extensins in Arabidopsis
RT and rice. A conserved family of cell wall proteins form a vegetative and a
RT reproductive clade.";
RL Plant Physiol. 131:1313-1326(2003).
CC -!- FUNCTION: Modulates cell morphogenesis by regulating cell wall
CC formation and assembly, and/or growth polarization. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O81765-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O81765-2; Sequence=VSP_039479;
CC -!- TISSUE SPECIFICITY: Expressed in flowers, stamen, pollen, and
CC pollinated carpels. {ECO:0000269|PubMed:12644681}.
CC -!- PTM: Hydroxylated on proline residues in the S-P-P-P-P repeat.
CC {ECO:0000250}.
CC -!- PTM: O-glycosylated on hydroxyprolines. {ECO:0000250}.
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DR EMBL; AL031032; CAA19879.1; -; Genomic_DNA.
DR EMBL; AL161584; CAB80114.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86302.1; -; Genomic_DNA.
DR EMBL; DQ446892; ABE66109.1; -; mRNA.
DR PIR; T05225; T05225.
DR RefSeq; NP_195123.1; NM_119558.3. [O81765-1]
DR AlphaFoldDB; O81765; -.
DR SMR; O81765; -.
DR STRING; 3702.AT4G33970.1; -.
DR TCDB; 3.A.20.1.2; the peroxisomal protein importer (ppi) family.
DR PaxDb; O81765; -.
DR PRIDE; O81765; -.
DR ProteomicsDB; 236639; -. [O81765-1]
DR EnsemblPlants; AT4G33970.1; AT4G33970.1; AT4G33970. [O81765-1]
DR GeneID; 829543; -.
DR Gramene; AT4G33970.1; AT4G33970.1; AT4G33970. [O81765-1]
DR KEGG; ath:AT4G33970; -.
DR Araport; AT4G33970; -.
DR TAIR; locus:2118949; AT4G33970.
DR eggNOG; ENOG502QRPA; Eukaryota.
DR HOGENOM; CLU_000288_23_3_1; -.
DR InParanoid; O81765; -.
DR OMA; CSKHVCP; -.
DR OrthoDB; 725535at2759; -.
DR PhylomeDB; O81765; -.
DR PRO; PR:O81765; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O81765; baseline and differential.
DR Genevisible; O81765; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0005199; F:structural constituent of cell wall; ISS:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR032675; LRR_dom_sf.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall; Cell wall biogenesis/degradation;
KW Glycoprotein; Hydroxylation; Leucine-rich repeat; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..39
FT /evidence="ECO:0000255"
FT CHAIN 40..699
FT /note="Pollen-specific leucine-rich repeat extensin-like
FT protein 4"
FT /id="PRO_0000395471"
FT REPEAT 133..157
FT /note="LRR 1"
FT REPEAT 158..180
FT /note="LRR 2"
FT REPEAT 182..205
FT /note="LRR 3"
FT REPEAT 206..229
FT /note="LRR 4"
FT REPEAT 231..251
FT /note="LRR 5"
FT REPEAT 253..275
FT /note="LRR 6"
FT REPEAT 276..299
FT /note="LRR 7"
FT REPEAT 301..323
FT /note="LRR 8"
FT REPEAT 324..347
FT /note="LRR 9"
FT REGION 411..699
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..699
FT /note="Contains the Ser-Pro(4) repeats"
FT COMPBIAS 423..504
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..666
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 301
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 352
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 104..584
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17147637"
FT /id="VSP_039479"
SQ SEQUENCE 699 AA; 75248 MW; 1D1162926FE1D58D CRC64;
MPFYKQPWVF SKVFVLAMAK PPSFGCCFFL LFFSFLSSSF VSFALTDTEA AFIVQRQLLT
LPDNGELPDD IEYEVDLKAT FANTRLKRAY IALQAWKKAI FSDPFNTTGN WHGPHVCGYT
GVVCAPALDD SDVTVVAGVD LNGADIAGHL PAELGLMTDV AMFHLNSNRF CGIIPKSFEK
LKLMHEFDVS NNRFVGPFPN VVLSWPDVKY FDLRFNDFEG QVPPELFKKE LDAIFLNDNR
FTSVIPESLG ESPASVVTFA NNKFTGCIPK SIGNMKNLNE IVFMDNDLGG CFPSEIGKLS
NVTVFDASKN SFIGRLPTSF VGLTSVEEID ISGNKLTGLV PHNICQLPNL VNLTYSYNYF
SGQGGSCVPG GSRKEIALDD TRNCLASRPE QRSAQECAVV INRPVDCSKD KCAGGSSTPS
KPSPVHKPTP VPTTPVHKPT PVPTTPVQKP SPVPTTPVQK PSPVPTTPVH EPSPVLATPV
DKPSPVPSRP VQKPQPPKES PQPDDPYDQS PVTKRRSPPP APVNSPPPPV YSPPPPPPPV
HSPPPPVHSP PPPPVYSPPP PPPPVHSPPP PVFSPPPPVY SPPPPVHSPP PPVHSPPPPA
PVHSPPPPVH SPPPPPPVYS PPPPVFSPPP SQSPPVVYSP PPRPPKINSP PVQSPPPAPV
EKKETPPAHA PAPSDDEFII PPFIGHQYAS PPPPMFAGY
