ID   PLS1_PHYBI              Reviewed;          66 AA.
AC   Q800R3;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Phylloseptin-B1 {ECO:0000303|PubMed:18644413};
DE            Short=PLS-B1 {ECO:0000303|PubMed:18644413};
DE   AltName: Full=Dermaseptin PBN1 {ECO:0000303|PubMed:15222751};
DE            Short=DRP-PBN1 {ECO:0000303|PubMed:15222751};
DE   Flags: Precursor;
OS   Phyllomedusa bicolor (Two-colored leaf frog) (Rana bicolor).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=8393;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Skin;
RX   PubMed=12709067; DOI=10.1046/j.1432-1033.2003.03584.x;
RA   Vanhoye D., Bruston F., Nicolas P., Amiche M.;
RT   "Antimicrobial peptides from hylid and ranin frogs originated from a 150-
RT   million-year-old ancestral precursor with a conserved signal peptide but a
RT   hypermutable antimicrobial domain.";
RL   Eur. J. Biochem. 270:2068-2081(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AMIDATION AT LEU-65, AND SYNTHESIS OF
RP   47-66.
RC   TISSUE=Skin;
RX   PubMed=15222751; DOI=10.1021/bi0493158;
RA   Vanhoye D., Bruston F., El Amri S., Ladram A., Amiche M., Nicolas P.;
RT   "Membrane association, electrostatic sequestration, and cytotoxicity of
RT   Gly-Leu-rich peptide orthologs with differing functions.";
RL   Biochemistry 43:8391-8409(2004).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=18644413; DOI=10.1016/j.peptides.2008.06.017;
RA   Amiche M., Ladram A., Nicolas P.;
RT   "A consistent nomenclature of antimicrobial peptides isolated from frogs of
RT   the subfamily Phyllomedusinae.";
RL   Peptides 29:2074-2082(2008).
CC   -!- FUNCTION: Antimicrobial peptide with activity against only a few
CC       strains of Gram-positive bacteria (S.aureus and B.megaterium)
CC       (PubMed:15222751). Acts in a synergistic effect in combination with
CC       Plasticin-B1 at doses that are not active alone (PubMed:15222751).
CC       {ECO:0000269|PubMed:15222751}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:15222751}. Target
CC       cell membrane {ECO:0000305|PubMed:15222751}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:15222751}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Phylloseptin subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=00911";
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DR   EMBL; AY218784; AAO62959.1; -; mRNA.
DR   AlphaFoldDB; Q800R3; -.
DR   TCDB; 1.C.52.1.10; the dermaseptin (dermaseptin) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR016322; FSAP.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
DR   PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Immunity; Innate immunity; Membrane;
KW   Secreted; Signal; Target cell membrane; Target membrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..46
FT                   /evidence="ECO:0000305|PubMed:15222751"
FT                   /id="PRO_0000449897"
FT   PEPTIDE         47..65
FT                   /note="Phylloseptin-B1"
FT                   /evidence="ECO:0000305|PubMed:15222751"
FT                   /id="PRO_5004298810"
FT   MOD_RES         65
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000305|PubMed:15222751"
SQ   SEQUENCE   66 AA;  7467 MW;  A95E305A92111294 CRC64;
     MAFLKKSLFL VLFLGLVSLS ICEEEKRETE EKEYDQGEDD KSEEKRFLSL IPHIVSGVAA
     LAKHLG