ID   PLS2_PITNO              Reviewed;          18 AA.
AC   P0DTT7;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=Phylloseptin-N2 {ECO:0000305};
DE            Short=PLS-N2 {ECO:0000305};
DE   AltName: Full=Phylloseptin-H8b {ECO:0000303|PubMed:23774944};
DE            Short=PLS-H8b {ECO:0000303|PubMed:23774944};
OS   Pithecopus nordestinus (Northeastern Brazilian leaf frog) (Phyllomedusa
OS   nordestina).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=2034992;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND SYNTHESIS.
RX   PubMed=23774944; DOI=10.3390/molecules18067058;
RA   Brand G.D., Santos R.C., Arake L.M., Silva V.G., Veras L.M., Costa V.,
RA   Costa C.H., Kuckelhaus S.S., Alexandre J.G., Feio M.J., Leite J.R.;
RT   "The skin secretion of the amphibian Phyllomedusa nordestina: a source of
RT   antimicrobial and antiprotozoal peptides.";
RL   Molecules 18:7058-7070(2013).
CC   -!- FUNCTION: Antimicrobial peptide with low activity against Leishmania
CC       species (L.amazonensis and L.infantum) (PubMed:23774944). Shows low
CC       cytotoxicity against mammalian cells in models of peritoneal
CC       macrophages (PubMed:23774944). {ECO:0000269|PubMed:23774944}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23774944}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:23774944}.
CC   -!- PTM: One shorter peptide due to unspecific cleavage of this peptide has
CC       also been identified. {ECO:0000269|PubMed:23774944}.
CC   -!- MASS SPECTROMETRY: Mass=1858.23; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23774944};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Phylloseptin subfamily. {ECO:0000305}.
CC   -!- CAUTION: Sequence shown in this entry in copied from Fig.2, and not
CC       from Table 1, which differ. {ECO:0000305|PubMed:23774944}.
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DR   AlphaFoldDB; P0DTT7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antimicrobial; Direct protein sequencing;
KW   Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..18
FT                   /note="Phylloseptin-N2"
FT                   /evidence="ECO:0000269|PubMed:23774944"
FT                   /id="PRO_0000449577"
SQ   SEQUENCE   18 AA;  1858 MW;  E726B5DDAFE8BEE2 CRC64;
     FLSLIPSLVS GAVSLVKK