PLS31_PHYTB
ID PLS31_PHYTB Reviewed; 19 AA.
AC C0HLD8;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 1.
DT 25-MAY-2022, entry version 7.
DE RecName: Full=Phylloseptin-3.1TR {ECO:0000303|PubMed:29980138};
DE Short=PLS-3.1TR {ECO:0000305};
DE AltName: Full=Phylloseptin-3.3TR {ECO:0000303|PubMed:29980138};
OS Phyllomedusa trinitatis (Trinidad leaf frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC Phyllomedusa.
OX NCBI_TaxID=332092;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SYNTHESIS, SUBCELLULAR LOCATION, MASS
RP SPECTROMETRY, AND AMIDATION AT LEU-19.
RC TISSUE=Skin secretion;
RX PubMed=29980138; DOI=10.1016/j.cbd.2018.06.006;
RA Mechkarska M., Coquet L., Leprince J., Auguste R.J., Jouenne T.,
RA Mangoni M.L., Conlon J.M.;
RT "Peptidomic analysis of the host-defense peptides in skin secretions of the
RT Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae).";
RL Comp. Biochem. Physiol. 28:72-79(2018).
RN [2]
RP FUNCTION, AND SYNTHESIS.
RX PubMed=30734396; DOI=10.1002/psc.3153;
RA Pantic J., Guilhaudis L., Musale V., Attoub S., Lukic M.L., Mechkarska M.,
RA Conlon J.M.;
RT "Immunomodulatory, insulinotropic, and cytotoxic activities of
RT phylloseptins and plasticin-TR from the Trinidanian leaf frog Phyllomedusa
RT trinitatis.";
RL J. Pept. Sci. 25:E3153-E3153(2019).
CC -!- FUNCTION: The amidated Phylloseptin-3.1TR has weak antimicrobial
CC activity against Gram-negative bacterium E.coli ATCC 25922 (MIC=100
CC uM), Gram-positive bacterium S.epidermidis ATCC 12228 (MIC=12.5 uM) and
CC against fungus C.albicans ATCC 24433 (MIC=25 uM) (PubMed:29980138). Has
CC an anti-inflammatory effect, since it inhibits the production of the
CC pro-inflammatory cytokines TNF-alpha, and induces the production of the
CC anti-inflammatory cytokine IL-10 (PubMed:30734396). Has an activity of
CC stimulation of insulin release, which may protect the species from
CC being eaten by predators by causing fatal hypoglycemia
CC (PubMed:30734396). Is cytotoxic to cancer line cells (PubMed:30734396).
CC Shows moderate hemolysis on mouse erythrocytes (LC(50)=12 uM)
CC (PubMed:30734396). {ECO:0000269|PubMed:29980138,
CC ECO:0000269|PubMed:30734396}.
CC -!- FUNCTION: The non-amidated Phylloseptin-3.3TR has weak antimicrobial
CC activity against fungus C.albicans ATCC 24433 (MIC=100 uM). Not active
CC against Gram-negative bacterium E.coli ATCC 25922 and Gram-positive
CC bacterium S.epidermidis ATCC 12228 at concentrations up to 100 uM
CC (PubMed:29980138). Has an anti-inflammatory effect, since it inhibits
CC the production of the pro-inflammatory cytokines TNF-alpha and IL-1
CC beta, and induces the production of the anti-inflammatory cytokine IL-
CC 10 (PubMed:30734396). Has high activity of stimulation of insulin
CC release, which may protect the species from being eaten by predators by
CC causing fatal hypoglycemia (PubMed:30734396). Is not cytotoxic to
CC cancer line cells (PubMed:30734396). Shows very low hemolysis on mouse
CC erythrocytes (LC(50)=340 uM) (PubMed:30734396).
CC {ECO:0000269|PubMed:29980138, ECO:0000269|PubMed:30734396}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29980138}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000305|PubMed:29980138}.
CC -!- PTM: Phylloseptin-3.1TR is amidated, whereas Phylloseptin-3.3TR is the
CC name given to the non-amidated form. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=2049.3; Method=MALDI; Note=amidated
CC Phylloseptin-3.1TR.; Evidence={ECO:0000269|PubMed:29980138};
CC -!- MASS SPECTROMETRY: Mass=2050.1; Method=MALDI; Note=non-amidated
CC Phylloseptin-3.3TR.; Evidence={ECO:0000269|PubMed:29980138};
CC -!- PHARMACEUTICAL: Phylloseptin-3.3TR may represent a template for the
CC design of potent anti-inflammatory agents for use in the control
CC hyperinflammatory phase of sepsis, since it has the ability to inhibit
CC production of TNF-alpha and IL-1 beta and to stimulate IL-10 production
CC by peritoneal cells coupled with very low toxicity against mouse
CC erythrocytes and A549 human lung adenocarcinoma cells.
CC {ECO:0000305|PubMed:30734396}.
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Phylloseptin subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC URL="https://wangapd3.com/database/query_output.php?ID=02992";
CC -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC URL="https://wangapd3.com/database/query_output.php?ID=02994";
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DR AlphaFoldDB; C0HLD8; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW Direct protein sequencing; Fungicide; Hemolysis; Immunity; Innate immunity;
KW Pharmaceutical; Secreted.
FT PEPTIDE 1..19
FT /note="Phylloseptin-3.1TR"
FT /evidence="ECO:0000269|PubMed:29980138"
FT /id="PRO_0000445198"
FT MOD_RES 19
FT /note="Leucine amide; in form Phylloseptin-3.1TR"
FT /evidence="ECO:0000269|PubMed:29980138"
SQ SEQUENCE 19 AA; 2051 MW; 55BBF25A92D7F7A2 CRC64;
FFSMIPKIAT GIASLVKNL
